Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EL148_RS06890 Genome accession   NZ_LR134364
Coordinates   1432599..1433045 (-) Length   148 a.a.
NCBI ID   WP_064081943.1    Uniprot ID   -
Organism   [Haemophilus] ducreyi strain NCTC11483     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1427599..1438045
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL148_RS06860 (NCTC11483_01440) yacG 1428545..1428733 (-) 189 WP_064081939.1 DNA gyrase inhibitor YacG -
  EL148_RS06865 (NCTC11483_01441) coaE 1428736..1429353 (-) 618 WP_064081940.1 dephospho-CoA kinase -
  EL148_RS06870 (NCTC11483_01442) - 1429371..1429754 (-) 384 WP_335673639.1 prepilin peptidase -
  EL148_RS06875 (NCTC11483_01443) - 1429747..1430016 (-) 270 WP_078179334.1 hypothetical protein -
  EL148_RS06880 (NCTC11483_01444) pilC 1430016..1431209 (-) 1194 WP_064081941.1 type II secretion system F family protein Machinery gene
  EL148_RS06885 (NCTC11483_01445) pilB 1431193..1432587 (-) 1395 WP_064081942.1 GspE/PulE family protein Machinery gene
  EL148_RS06890 (NCTC11483_01446) pilA 1432599..1433045 (-) 447 WP_064081943.1 pilin Machinery gene
  EL148_RS06895 (NCTC11483_01447) radA 1433239..1434618 (+) 1380 WP_064081944.1 DNA repair protein RadA -
  EL148_RS06900 (NCTC11483_01448) - 1434685..1434900 (-) 216 WP_010945033.1 YgjV family protein -
  EL148_RS06905 (NCTC11483_01449) - 1435022..1435507 (-) 486 WP_064081945.1 dihydrofolate reductase -
  EL148_RS06910 (NCTC11483_01450) - 1435628..1436398 (+) 771 WP_064082999.1 TSUP family transporter -
  EL148_RS06915 (NCTC11483_01451) nth 1436407..1437042 (+) 636 WP_010945029.1 endonuclease III -
  EL148_RS06920 (NCTC11483_01452) fdhD 1437102..1437929 (-) 828 WP_064081946.1 formate dehydrogenase accessory sulfurtransferase FdhD -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15856.17 Da        Isoelectric Point: 7.5799

>NTDB_id=1122305 EL148_RS06890 WP_064081943.1 1432599..1433045(-) (pilA) [[Haemophilus] ducreyi strain NCTC11483]
MSSLFLIKPLQKAFTLIELMIVIAIIAILATVAIPSYNSYTKKAALSELLAASSAYKTDVEICVYNLGRTDGCNAGTNGI
KNDNSGKSKYVGSISVENGTITVTGKESLKDYSYKMTPTLQGESLTWKTTCNGADTSLFPANFCSTEQ

Nucleotide


Download         Length: 447 bp        

>NTDB_id=1122305 EL148_RS06890 WP_064081943.1 1432599..1433045(-) (pilA) [[Haemophilus] ducreyi strain NCTC11483]
ATGTCATCTTTATTTTTGATTAAGCCACTACAAAAGGCGTTTACTTTAATTGAATTAATGATTGTGATTGCCATTATCGC
AATTTTAGCTACGGTTGCGATTCCTTCTTACAATAGTTATACCAAAAAAGCTGCGTTATCTGAGTTATTAGCCGCTTCAT
CAGCGTATAAAACCGATGTAGAAATATGTGTTTATAATTTGGGTAGAACAGATGGATGTAATGCAGGAACAAACGGTATA
AAAAATGATAATTCAGGTAAAAGTAAATATGTCGGATCAATCAGTGTAGAAAATGGAACGATTACAGTAACGGGTAAAGA
AAGCCTTAAAGATTATAGTTATAAAATGACACCAACCTTACAAGGCGAATCCTTAACTTGGAAAACGACTTGTAATGGTG
CTGATACTAGCCTTTTCCCTGCTAATTTTTGTTCTACTGAACAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(43-142)

Prokaryotic N-terminal methylation motif (N_methyl)

(11-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

61.486

100

0.615
  pilA Haemophilus influenzae 86-028NP

57.246

93.243

0.534
  pilA Haemophilus influenzae Rd KW20

54.167

97.297

0.527

Multiple sequence alignment