Detailed information NTG1122062

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: EL327_RS16410
• Genome accession: NZ_LR134342
• Coordinates: 3416487..3417635 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain NCTC10728
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3411487..3422635

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL327_RS16385	-	3412638..3412934 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
EL327_RS16390 (NCTC10728_03210)	-	3412931..3413524 (-)	594	WP_003084544.1	YggT family protein	-
EL327_RS16395 (NCTC10728_03211)	proC	3413535..3414356 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
EL327_RS16400 (NCTC10728_03212)	-	3414368..3415060 (-)	693	WP_126438368.1	YggS family pyridoxal phosphate-dependent enzyme	-
EL327_RS16405 (NCTC10728_03213)	pilT	3415275..3416309 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
EL327_RS16410 (NCTC10728_03214)	pilU	3416487..3417635 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
EL327_RS16415 (NCTC10728_03215)	czcD	3417643..3418542 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
EL327_RS16420 (NCTC10728_03216)	-	3418696..3419100 (+)	405	WP_003084560.1	NINE protein	-
EL327_RS16425 (NCTC10728_03217)	-	3419343..3420716 (+)	1374	WP_003160915.1	pyridoxal-phosphate dependent enzyme	-
EL327_RS16430 (NCTC10728_03218)	-	3420713..3421897 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=1122062 EL327_RS16410 WP_003100959.1 3416487..3417635(+) (pilU) [Pseudomonas aeruginosa strain NCTC10728]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1122062 EL327_RS16410 WP_003100959.1 3416487..3417635(+) (pilU) [Pseudomonas aeruginosa strain NCTC10728]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382