Detailed information NTG1122055

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: EL327_RS11475
• Genome accession: NZ_LR134342
• Coordinates: 2380793..2381857 (-)
• Length: 354 a.a.
• NCBI ID: WP_034024943.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain NCTC10728
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2375793..2386857

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL327_RS11450 (NCTC10728_02240)	aroK	2376340..2376858 (-)	519	WP_003095833.1	shikimate kinase AroK	-
EL327_RS11455 (NCTC10728_02241)	pilQ	2376870..2379002 (-)	2133	WP_003116137.1	type 4a pilus secretin PilQ	Machinery gene
EL327_RS11460 (NCTC10728_02242)	pilP	2379056..2379580 (-)	525	WP_003116136.1	type 4a pilus biogenesis lipoprotein PilP	-
EL327_RS11465 (NCTC10728_02243)	pilO	2379577..2380200 (-)	624	WP_003116135.1	type 4a pilus biogenesis protein PilO	-
EL327_RS11470 (NCTC10728_02244)	pilN	2380197..2380793 (-)	597	WP_003141783.1	type 4a pilus biogenesis protein PilN	-
EL327_RS11475 (NCTC10728_02245)	pilM	2380793..2381857 (-)	1065	WP_034024943.1	type IV pilus assembly protein PilM	Machinery gene
EL327_RS11480 (NCTC10728_02246)	-	2382042..2384510 (+)	2469	WP_003114576.1	penicillin-binding protein 1A	-
EL327_RS11485 (NCTC10728_02247)	-	2384615..2385883 (-)	1269	WP_003103278.1	malic enzyme	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37997.51 Da
• Isoelectric Point: 4.5446

>NTDB_id=1122055 EL327_RS11475 WP_034024943.1 2380793..2381857(-) (pilM) [Pseudomonas aeruginosa strain NCTC10728]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVEVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=1122055 EL327_RS11475 WP_034024943.1 2380793..2381857(-) (pilM) [Pseudomonas aeruginosa strain NCTC10728]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGACTCTCCGCGC
GCAATCCCGAGCGCGTGGAGGTACTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.899	100	0.562
comM	Acinetobacter nosocomialis M2	55.899	100	0.562
comM	Acinetobacter baylyi ADP1	51.977	100	0.52
pilM	Legionella pneumophila strain ERS1305867	46.328	100	0.463