Detailed information NTG1121647

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: EL342_RS10885
• Genome accession: NZ_LR134330
• Coordinates: 2295993..2297141 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain NCTC13715
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2290993..2302141

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL342_RS10860	-	2292144..2292440 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
EL342_RS10865 (NCTC13715_02114)	-	2292437..2293030 (-)	594	WP_003084544.1	YggT family protein	-
EL342_RS10870 (NCTC13715_02115)	proC	2293041..2293862 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
EL342_RS10875 (NCTC13715_02116)	-	2293874..2294566 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
EL342_RS10880 (NCTC13715_02117)	pilT	2294781..2295815 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
EL342_RS10885 (NCTC13715_02118)	pilU	2295993..2297141 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
EL342_RS10890 (NCTC13715_02119)	czcD	2297149..2298048 (-)	900	WP_023913256.1	CDF family zinc transporter CzcD	-
EL342_RS10895 (NCTC13715_02120)	-	2298203..2298607 (+)	405	WP_003084560.1	NINE protein	-
EL342_RS10900 (NCTC13715_02121)	-	2298850..2300223 (+)	1374	WP_023098360.1	pyridoxal-phosphate dependent enzyme	-
EL342_RS10905 (NCTC13715_02122)	-	2300220..2301404 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=1121647 EL342_RS10885 WP_003110016.1 2295993..2297141(+) (pilU) [Pseudomonas aeruginosa strain NCTC13715]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1121647 EL342_RS10885 WP_003110016.1 2295993..2297141(+) (pilU) [Pseudomonas aeruginosa strain NCTC13715]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGATCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382