Detailed information NTG1121640

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: EL342_RS05580
• Genome accession: NZ_LR134330
• Coordinates: 1186547..1187611 (-)
• Length: 354 a.a.
• NCBI ID: WP_003110888.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain NCTC13715
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1181547..1192611

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL342_RS05555 (NCTC13715_01083)	aroK	1182094..1182612 (-)	519	WP_003095833.1	shikimate kinase AroK	-
EL342_RS05560 (NCTC13715_01084)	pilQ	1182624..1184756 (-)	2133	WP_023100018.1	type 4a pilus secretin PilQ	Machinery gene
EL342_RS05565 (NCTC13715_01085)	pilP	1184810..1185334 (-)	525	WP_003116136.1	type 4a pilus biogenesis lipoprotein PilP	-
EL342_RS05570 (NCTC13715_01086)	pilO	1185331..1185954 (-)	624	WP_003116135.1	type 4a pilus biogenesis protein PilO	-
EL342_RS05575 (NCTC13715_01087)	pilN	1185951..1186547 (-)	597	WP_003141783.1	type 4a pilus biogenesis protein PilN	-
EL342_RS05580 (NCTC13715_01088)	pilM	1186547..1187611 (-)	1065	WP_003110888.1	type IV pilus assembly protein PilM	Machinery gene
EL342_RS05585 (NCTC13715_01089)	-	1187796..1190267 (+)	2472	WP_003135771.1	penicillin-binding protein 1A	-
EL342_RS05590 (NCTC13715_01090)	-	1190363..1191631 (-)	1269	WP_003095842.1	malic enzyme	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37983.48 Da
• Isoelectric Point: 4.5405

>NTDB_id=1121640 EL342_RS05580 WP_003110888.1 1186547..1187611(-) (pilM) [Pseudomonas aeruginosa strain NCTC13715]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=1121640 EL342_RS05580 WP_003110888.1 1186547..1187611(-) (pilM) [Pseudomonas aeruginosa strain NCTC13715]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGACTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGCCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.899	100	0.562
comM	Acinetobacter nosocomialis M2	55.899	100	0.562
comM	Acinetobacter baylyi ADP1	51.977	100	0.52
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466