Detailed information NTG1121344

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: EL233_RS01285
• Genome accession: NZ_LR134319
• Coordinates: 290167..291312 (+)
• Length: 381 a.a.
• NCBI ID: WP_019405534.1
• Uniprot ID: -
• Organism: Stutzerimonas stutzeri strain NCTC10450
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 285167..296312

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL233_RS01265 (NCTC10450_00253)	-	286882..287472 (-)	591	WP_014598006.1	YggT family protein	-
EL233_RS01270 (NCTC10450_00254)	proC	287482..288306 (-)	825	WP_126525734.1	pyrroline-5-carboxylate reductase	-
EL233_RS01275 (NCTC10450_00255)	-	288317..289006 (-)	690	WP_011914971.1	YggS family pyridoxal phosphate-dependent enzyme	-
EL233_RS01280 (NCTC10450_00256)	pilT	289073..290107 (+)	1035	WP_011914970.1	type IV pilus twitching motility protein PilT	Machinery gene
EL233_RS01285 (NCTC10450_00257)	pilU	290167..291312 (+)	1146	WP_019405534.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
EL233_RS01290 (NCTC10450_00258)	-	291309..292142 (-)	834	WP_126525736.1	TIGR03915 family putative DNA repair protein	-
EL233_RS01295 (NCTC10450_00259)	-	292142..293356 (-)	1215	WP_126525738.1	putative DNA modification/repair radical SAM protein	-
EL233_RS01300 (NCTC10450_00260)	-	293492..293887 (+)	396	WP_011914966.1	NINE protein	-
EL233_RS01305 (NCTC10450_00261)	-	293945..295219 (-)	1275	WP_126525740.1	dihydroorotase	-
EL233_RS01310 (NCTC10450_00262)	-	295219..296235 (-)	1017	WP_011914964.1	aspartate carbamoyltransferase catalytic subunit	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42566.88 Da
• Isoelectric Point: 6.8858

>NTDB_id=1121344 EL233_RS01285 WP_019405534.1 290167..291312(+) (pilU) [Stutzerimonas stutzeri strain NCTC10450]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVSKTPMSPEMTRETVHGVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTIEELKLPDVLKKLSMTKRGLVLFVGATGTGKSTSLASMIGYRNKNSSGHIISIED
PIEFIHQHQNCIVTQREVGIDTSSFEVALKNTLRQAPDVILIGEIRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPPDRHNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTELGMQTFDQA
LYNLYVQGEITYEDALLHADSANDLRLLIKLGSETDGEHLTSVSQGLSLEVSDEDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=1121344 EL233_RS01285 WP_019405534.1 290167..291312(+) (pilU) [Stutzerimonas stutzeri strain NCTC10450]
ATGGAATTCGAGAAACTTCTGCGCCTGATGGTCGAAAAAGGCGGCTCCGATCTGTTCATCACGGCTGGCGTGCCGCCATC
GATGAAGGTCAACGGCAAGATCATGCCGGTGAGCAAGACGCCCATGTCGCCGGAGATGACCCGGGAAACCGTGCACGGCG
TGATGAACGAGCAGCAGCGCCGCGAGTTCACCGAGAACCACGAATGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGTGTCAGCGCGTTCTATCAGCGAAACCTGGCCGGCATGGTGCTGCGTCGCATCGAGACCAACATCCCGACCATCGA
AGAACTCAAGCTGCCGGACGTCCTGAAGAAGCTGTCGATGACCAAACGTGGTCTGGTGCTGTTCGTCGGCGCCACCGGTA
CCGGCAAGTCGACATCGCTGGCCTCGATGATCGGCTACCGCAACAAGAACTCCAGCGGGCACATCATCTCCATCGAAGAC
CCGATCGAATTCATCCACCAGCACCAGAATTGCATCGTCACCCAGCGCGAGGTGGGCATCGACACCAGCTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTCATCGGCGAGATCCGTACCCGCGAAACCATGGATTACG
CGGTCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGCCGGACCGGCACAACCAGGTCTGGATGGACCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTTCCGACCCCGGACGGCAAGGGTCGTCGTGCGGTGATCGAGGTGCTGATCAATACGCCGCTGGCGGCCGACCTGA
TCCGCAAGGGGGAGGTGCACGAGCTGAAATCGCTGATGAAGCGCTCCACCGAGCTGGGTATGCAGACCTTCGACCAAGCG
CTGTACAACCTCTACGTGCAGGGCGAGATCACCTACGAAGATGCCCTGCTGCATGCTGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGGGTTCGGAAACCGACGGTGAGCACCTGACCAGCGTATCCCAGGGCCTGAGCCTGGAGGTCAGCGACG
AGGACCCGGGCCGGCGCTTCCGTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	95.013	100	0.95
pilU	Acinetobacter baylyi ADP1	66.106	93.701	0.619
pilU	Vibrio cholerae strain A1552	59.429	91.864	0.546
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.953	88.976	0.391