Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   EL257_RS23070 Genome accession   NZ_LR134318
Coordinates   5066326..5067672 (+) Length   448 a.a.
NCBI ID   WP_126366495.1    Uniprot ID   A0A3S4PAX1
Organism   Pseudomonas fluorescens strain NCTC9428     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5061326..5072672
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL257_RS23045 (NCTC9428_04596) pgeF 5061541..5062263 (-) 723 WP_126366489.1 peptidoglycan editing factor PgeF -
  EL257_RS23050 (NCTC9428_04597) rluD 5062260..5063222 (-) 963 WP_007909858.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  EL257_RS23055 (NCTC9428_04598) - 5063374..5064390 (+) 1017 WP_126366491.1 outer membrane protein assembly factor BamD -
  EL257_RS23060 (NCTC9428_04599) - 5064517..5064750 (+) 234 WP_126366493.1 PP0621 family protein -
  EL257_RS23065 (NCTC9428_04600) pilS 5064740..5066329 (+) 1590 WP_126368242.1 sensor histidine kinase Regulator
  EL257_RS23070 (NCTC9428_04601) pilR 5066326..5067672 (+) 1347 WP_126366495.1 sigma-54-dependent transcriptional regulator Regulator
  EL257_RS23075 (NCTC9428_04602) thiO 5067823..5068923 (-) 1101 WP_126366497.1 glycine oxidase ThiO -
  EL257_RS23080 (NCTC9428_04603) - 5068981..5069382 (-) 402 WP_126366499.1 type IV pilin protein -
  EL257_RS23085 (NCTC9428_04604) - 5069398..5072487 (-) 3090 WP_126366501.1 pilus assembly protein -

Sequence

Protein


Download         Length: 448 a.a.        Molecular weight: 50143.51 Da        Isoelectric Point: 6.6633

>NTDB_id=1121331 EL257_RS23070 WP_126366495.1 5066326..5067672(+) (pilR) [Pseudomonas fluorescens strain NCTC9428]
MNTSPRQKILIVDDEPDIRELLEITLGRMKLDTHSARNLAEAQALLHREPFDLCLTDMRLPDGTGLELVQHIQQRYPQLP
VAMITAYGSLETAINALKAGAFDFLTKPVDLTRLRELVSAALRMPAAGGVCTLIDRRLLGDSAPMRNLRKQIDKLARSQA
PVYISGESGSGKELVARLIHEQGPRAGQPFVPVNCGAIPSELMESEFFGHRKGSFTGAVEDKPGLFQAAHGGTLFLDEVA
DLPLSMQVKLLRAIQEKAIRSVGGQQEIVVDVRVLCATHKDLDAEVAAERFRQDLYYRLNVIELRVPSLRERRDDIEALA
THVLKRLARDSGQPEARLHPQALEALKNYRFPGNVRELENVLERAHTLCEQRTIEAQDLRLSEGNCSADGALADLTQIDN
LEDYLESVERKLILQALEETRWNRTAAAQRLSLSFRSMRYRLKKLGLD

Nucleotide


Download         Length: 1347 bp        

>NTDB_id=1121331 EL257_RS23070 WP_126366495.1 5066326..5067672(+) (pilR) [Pseudomonas fluorescens strain NCTC9428]
TTGAACACGAGCCCACGGCAAAAAATCCTCATCGTCGACGACGAGCCCGACATCCGCGAACTCCTGGAAATCACTTTAGG
CCGGATGAAACTTGACACTCATAGCGCGCGCAATCTGGCCGAAGCCCAGGCGCTGCTGCACCGCGAACCATTCGACTTGT
GCCTGACCGACATGCGTCTGCCCGACGGCACGGGACTTGAGCTGGTGCAACACATTCAGCAGCGTTATCCACAACTGCCG
GTGGCGATGATCACCGCGTACGGCAGTCTGGAAACCGCGATCAATGCGCTTAAAGCCGGGGCGTTCGATTTCCTCACCAA
ACCGGTCGACCTTACACGCCTGCGCGAACTGGTCAGCGCGGCCCTGCGCATGCCGGCAGCCGGCGGCGTGTGCACATTAA
TTGATCGGCGCTTGCTCGGTGACTCGGCGCCGATGCGCAATCTGCGCAAGCAGATCGACAAACTCGCCCGCAGTCAGGCG
CCGGTCTACATCAGCGGTGAATCCGGCAGCGGTAAAGAGCTGGTGGCGCGGCTGATCCACGAGCAAGGCCCGCGCGCTGG
CCAGCCATTCGTACCAGTGAACTGCGGGGCGATTCCGTCGGAGCTGATGGAAAGCGAGTTCTTCGGCCACCGCAAAGGCA
GTTTCACCGGCGCGGTCGAGGACAAACCGGGACTGTTTCAGGCAGCGCACGGCGGCACGTTGTTCCTCGATGAAGTGGCC
GACTTGCCGCTGTCGATGCAGGTGAAATTGCTTCGCGCGATTCAGGAAAAAGCCATTCGCAGCGTTGGTGGGCAACAGGA
AATCGTCGTCGATGTGCGGGTTCTTTGTGCGACACACAAGGATCTCGACGCCGAAGTGGCTGCCGAGCGCTTTCGCCAGG
ATCTGTATTACCGCTTGAACGTGATTGAGCTGCGCGTGCCGTCTCTGCGCGAGCGCCGCGACGACATCGAGGCTTTGGCC
ACGCACGTACTCAAACGTCTGGCCCGGGACAGCGGCCAACCCGAAGCGCGCCTGCATCCGCAGGCGCTGGAGGCGCTGAA
GAACTACCGGTTTCCCGGCAACGTACGCGAACTGGAGAATGTCCTCGAACGTGCACACACTTTGTGTGAACAGCGCACGA
TCGAAGCGCAGGATCTGCGCTTGAGCGAAGGGAATTGCTCGGCGGATGGCGCATTGGCCGACCTGACGCAGATCGATAAT
CTCGAGGATTATCTGGAGAGCGTGGAGCGCAAACTGATTTTGCAGGCCTTGGAAGAAACCCGCTGGAACCGCACGGCAGC
GGCGCAACGGTTGAGTCTTTCATTCAGGTCGATGCGTTATCGGCTGAAGAAATTGGGATTGGATTAG

Domains

Predicted by InterproScan.

Sigma-54 interaction domain (Sigma54_activat)

(139-304)

Response regulator receiver domain (Response_reg)

(9-118)

Bacterial regulatory protein, Fis family (HTH_8)

(405-445)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S4PAX1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

78.555

98.884

0.777
  pilR Acinetobacter baumannii strain A118

49.891

100

0.511
  luxO Vibrio cholerae strain A1552

38.202

99.33

0.379

Multiple sequence alignment