Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   EL257_RS23065 Genome accession   NZ_LR134318
Coordinates   5064740..5066329 (+) Length   529 a.a.
NCBI ID   WP_126368242.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain NCTC9428     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5059740..5071329
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL257_RS23045 (NCTC9428_04596) pgeF 5061541..5062263 (-) 723 WP_126366489.1 peptidoglycan editing factor PgeF -
  EL257_RS23050 (NCTC9428_04597) rluD 5062260..5063222 (-) 963 WP_007909858.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  EL257_RS23055 (NCTC9428_04598) - 5063374..5064390 (+) 1017 WP_126366491.1 outer membrane protein assembly factor BamD -
  EL257_RS23060 (NCTC9428_04599) - 5064517..5064750 (+) 234 WP_126366493.1 PP0621 family protein -
  EL257_RS23065 (NCTC9428_04600) pilS 5064740..5066329 (+) 1590 WP_126368242.1 sensor histidine kinase Regulator
  EL257_RS23070 (NCTC9428_04601) pilR 5066326..5067672 (+) 1347 WP_126366495.1 sigma-54-dependent transcriptional regulator Regulator
  EL257_RS23075 (NCTC9428_04602) thiO 5067823..5068923 (-) 1101 WP_126366497.1 glycine oxidase ThiO -
  EL257_RS23080 (NCTC9428_04603) - 5068981..5069382 (-) 402 WP_126366499.1 type IV pilin protein -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58493.26 Da        Isoelectric Point: 6.8241

>NTDB_id=1121330 EL257_RS23065 WP_126368242.1 5064740..5066329(+) (pilS) [Pseudomonas fluorescens strain NCTC9428]
MVAEAANADRKQAQRLLRLYHLYRLSVGITLVLLISSNMDNRLLTSANDDLLRGGSWLYLVLNILLAVFLENTRKPGQLF
SLALVDVLLLCGLFYAAGGVASALGNLLIVSVAISNTLLRRRIGLLIAAIGALGIVGLSFLLSFSHPLSANDYLQAGTLG
ALCFAASLLVQGLIRRLEASETLAEQRASEVVGLEALNALILQRMRTGILVLDAERRVQLANHSAESLLAQTHLQGHVID
DYFPALVDRLTMWMNNPTLRPQSLKIPGNGLELQPNFIALEQSPNQQTLVFLEDLAQIAQQAQQLKLAALGRLTAGISHE
IRNPLGAISHAAQLLGESEELDSADRRLTQIIQDHSKRMNRVIENVLQLSRRQQSAPQRLDLKPWLENFVAEAREQASER
QRIHLRINSGDFTTLMDPNQLTQILDNLLRNGWRHSALLHDQAEVWLAMFVDPDSQLAVLEVQDNGPGVPVDEQPHLFEP
FFTTSSQGTGLGLYLSRELCESNQARLDFKPRQGGGCFRITFAHGRKQS

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=1121330 EL257_RS23065 WP_126368242.1 5064740..5066329(+) (pilS) [Pseudomonas fluorescens strain NCTC9428]
GTGGTCGCTGAGGCCGCCAACGCCGACCGCAAACAGGCGCAGCGTCTGTTGCGCCTTTATCATCTCTATCGCTTAAGTGT
CGGCATCACCCTGGTGTTGCTGATTTCCAGCAACATGGACAACCGCCTGCTGACGTCGGCCAACGACGACCTGTTACGCG
GCGGCAGTTGGCTGTATCTCGTCCTCAACATTTTGCTGGCGGTGTTTCTTGAGAACACGCGCAAACCGGGCCAGCTGTTC
AGCCTGGCCCTCGTCGATGTTCTGTTGCTGTGCGGCCTGTTCTATGCGGCGGGTGGTGTGGCTAGTGCCCTCGGCAACCT
GCTGATTGTCTCGGTAGCGATCAGCAACACATTACTGCGTCGGCGTATCGGTCTGCTGATCGCGGCAATCGGCGCCCTCG
GTATTGTCGGGCTGAGTTTCCTGCTGAGTTTCAGCCATCCCCTGAGCGCCAACGATTACCTGCAGGCCGGCACCCTCGGC
GCGTTGTGCTTTGCCGCGTCGCTGCTGGTGCAAGGGCTGATCAGGCGCCTTGAGGCCAGCGAGACGCTGGCCGAGCAACG
CGCCAGTGAAGTCGTCGGGCTTGAAGCGCTCAATGCCTTGATCCTGCAACGCATGCGCACCGGTATTCTGGTGCTTGATG
CGGAGCGTCGGGTGCAATTGGCCAACCACAGTGCCGAGTCCCTGCTCGCGCAAACGCATCTGCAGGGGCACGTTATCGAC
GACTATTTCCCGGCCCTGGTCGACCGCCTGACAATGTGGATGAACAACCCTACCCTGCGCCCGCAAAGCCTGAAAATTCC
CGGTAACGGCCTGGAATTGCAGCCGAACTTCATCGCGCTCGAACAGAGCCCGAATCAGCAAACCCTGGTGTTTCTCGAGG
ATCTCGCGCAAATCGCCCAGCAGGCGCAACAACTCAAGCTCGCCGCGCTTGGTCGGCTGACGGCGGGGATTTCCCACGAG
ATCCGCAATCCGCTCGGCGCCATCAGTCATGCCGCGCAGCTGCTCGGCGAATCCGAGGAACTCGATAGCGCTGATCGGCG
TCTGACGCAGATTATTCAAGACCACTCCAAGCGCATGAACCGAGTCATCGAAAACGTCCTGCAACTGTCCCGCCGCCAGC
AAAGCGCTCCACAACGCCTGGACCTCAAGCCGTGGCTGGAAAACTTCGTTGCCGAAGCCCGCGAGCAGGCCAGCGAGCGC
CAACGGATTCACCTGCGGATCAATTCGGGAGACTTCACGACGCTGATGGACCCCAACCAGCTCACGCAAATTCTCGACAA
TCTCTTACGCAACGGCTGGCGTCACAGCGCCCTGCTGCACGATCAAGCCGAAGTCTGGCTGGCCATGTTCGTCGACCCCG
ACAGCCAGTTGGCGGTGCTCGAAGTGCAGGACAACGGCCCCGGCGTGCCTGTCGATGAACAGCCGCATCTTTTTGAACCG
TTCTTCACCACCAGCAGCCAAGGCACAGGTCTTGGGCTTTATCTGTCCCGTGAGCTGTGCGAAAGCAACCAAGCGCGCCT
AGACTTCAAACCACGCCAAGGCGGCGGCTGCTTTCGCATCACCTTTGCTCACGGACGGAAACAAAGTTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(309-374)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-522)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

60

100

0.601

Multiple sequence alignment