Detailed information NTG1121324

Bioinformatically predicted

Overview

• Name: comA
• Type: Machinery gene
• Locus tag: EL257_RS19730
• Genome accession: NZ_LR134318
• Coordinates: 4354239..4356473 (-)
• Length: 744 a.a.
• NCBI ID: WP_126365446.1
• Uniprot ID: A0A448DZX0
• Organism: Pseudomonas fluorescens strain NCTC9428
• Function: ssDNA transport through the inner membrane (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4349239..4361473

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL257_RS19695 (NCTC9428_03926)	murB	4349618..4350637 (-)	1020	WP_126365438.1	UDP-N-acetylmuramate dehydrogenase	-
EL257_RS19700 (NCTC9428_03927)	-	4350634..4351098 (-)	465	WP_126365440.1	low molecular weight protein-tyrosine-phosphatase	-
EL257_RS19705 (NCTC9428_03928)	kdsB	4351098..4351862 (-)	765	WP_126365442.1	3-deoxy-manno-octulosonate cytidylyltransferase	-
EL257_RS19710 (NCTC9428_03929)	-	4351859..4352044 (-)	186	WP_003174668.1	Trm112 family protein	-
EL257_RS19715 (NCTC9428_03930)	lpxK	4352067..4353077 (-)	1011	WP_126365444.1	tetraacyldisaccharide 4'-kinase	-
EL257_RS19720 (NCTC9428_03931)	-	4353077..4353505 (-)	429	WP_039760196.1	ExbD/TolR family protein	-
EL257_RS19725 (NCTC9428_03932)	exbB	4353502..4354137 (-)	636	WP_172604563.1	MotA/TolQ/ExbB proton channel family protein	Machinery gene
EL257_RS19730 (NCTC9428_03933)	comA	4354239..4356473 (-)	2235	WP_126365446.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
EL257_RS19735 (NCTC9428_03934)	-	4356610..4357128 (+)	519	WP_126365448.1	DUF2062 domain-containing protein	-
EL257_RS19740 (NCTC9428_03935)	-	4357293..4358072 (-)	780	WP_126365450.1	ABC transporter permease	-
EL257_RS19745 (NCTC9428_03936)	-	4358069..4359001 (-)	933	WP_126365452.1	ABC transporter ATP-binding protein	-
EL257_RS19750 (NCTC9428_03937)	-	4359134..4359757 (-)	624	WP_126365454.1	glutathione S-transferase family protein	-
EL257_RS19755 (NCTC9428_03938)	-	4359801..4360460 (-)	660	WP_126365456.1	transglutaminase-like domain-containing protein	-

Sequence

Protein

• Length: 744 a.a.
• Molecular weight: 81547.60 Da
• Isoelectric Point: 10.3300

>NTDB_id=1121324 EL257_RS19730 WP_126365446.1 4354239..4356473(-) (comA) [Pseudomonas fluorescens strain NCTC9428]
MRTGMMALAAGLLAPMFLPALPPVGLMMLLPLAGLALLALRRYRLALLLLGFGWACINAQWALNDRLAPSLDGETRWLEG
RVVGLPQNAGGVVRFEFADARSRQQEMPTLMRLAWYGGPPVSSGERWRLAVKLKRPAGLLNPDAFDYEAWLLAQRIGATG
TIKDGQRLSPARGAWRDSIRQRLLATDAQGRAGALAALVLGDGSGLSRDDWQILQDTGTVHLLVISGQHIGLLAALMYGL
VAGLARYGLWPLRWPWLPWACGMAFAAALGYGLLAGFDVPVQRACVMVGLVLLWRLRFRHLGAWWPLLLAFNGVLLFDPL
ASLRPGLWLSFAAVAVLIFTFGGRLGAWRWWQTWTRAQWLIAIGLCPVLLALNLPISLSGPLANLLAVPWVSLVVLPPAL
LGTLLLPVPYVGEGLLWLAGGLVDWLFRVLALIAGQWPAWTAPSISAWVWALGSFGAVLLLLPRGVPMRALGWPLLLILV
VPPRERLNEGLADVWQLDVGQGLAILVRTRHHTLLYDAGPRFGEFDLGARVVLPALRKLGVENLDLLLLSYADADHAGGA
LSIARGLKVARTISGDPPGLPAELNAQACESGQQWQWDGVRFQLWQWSDADDSNQRSCVLQIEANGERLLLTGDIDTHAE
RVLLDSPLAVPTHWLQSPHHGSRSSSSMALLKVLQPESVLISRGHGNSFGHPHPTVLARYRKQRLRIYDSAEHGAIHLQL
GTFSPPVMMRQQRRFWRDPPAPAR

Nucleotide

• Length: 2235 bp

>NTDB_id=1121324 EL257_RS19730 WP_126365446.1 4354239..4356473(-) (comA) [Pseudomonas fluorescens strain NCTC9428]
ATGCGCACAGGGATGATGGCGCTGGCAGCCGGTCTGCTGGCCCCGATGTTTTTACCGGCATTGCCGCCGGTCGGGTTGAT
GATGTTGTTGCCGCTGGCGGGTTTGGCCCTGCTGGCGTTGCGCAGGTATCGGTTGGCATTGCTGCTGTTGGGTTTCGGTT
GGGCGTGTATCAACGCGCAGTGGGCGCTGAATGATCGATTGGCGCCTTCGCTTGATGGCGAGACCCGTTGGCTCGAGGGG
CGCGTCGTCGGCTTGCCGCAAAACGCTGGCGGGGTAGTGCGTTTCGAATTCGCCGATGCGCGCTCGCGTCAGCAGGAAAT
GCCGACGCTGATGCGTCTGGCCTGGTACGGCGGACCACCGGTCAGTAGCGGCGAGCGCTGGCGACTGGCAGTCAAGCTCA
AGCGACCGGCAGGTTTGCTCAACCCGGACGCTTTCGATTACGAGGCATGGTTGTTGGCGCAACGCATCGGGGCGACGGGC
ACGATCAAGGATGGTCAGCGCCTGAGCCCAGCGCGCGGCGCCTGGCGTGACAGCATTCGCCAGCGCCTGTTGGCAACCGA
CGCGCAAGGCCGGGCAGGGGCGTTGGCTGCACTGGTGCTGGGCGACGGCTCAGGACTTAGTCGCGACGATTGGCAGATTC
TGCAGGACACCGGCACCGTGCATTTACTGGTGATATCCGGCCAGCACATCGGATTGCTGGCGGCGCTGATGTATGGCTTG
GTGGCCGGGCTCGCGCGTTACGGACTGTGGCCGTTGCGTTGGCCGTGGTTGCCGTGGGCCTGTGGAATGGCATTCGCGGC
AGCGCTCGGCTACGGCTTGCTCGCGGGTTTCGATGTGCCGGTGCAGCGCGCCTGTGTGATGGTCGGGCTGGTGTTGCTGT
GGCGTCTGCGCTTTCGTCATCTGGGTGCGTGGTGGCCGCTGCTGCTGGCGTTCAATGGCGTGTTGTTGTTCGATCCACTC
GCCAGTTTGCGTCCTGGCCTGTGGTTGTCGTTTGCAGCGGTGGCGGTGCTGATCTTCACTTTTGGCGGTCGTTTGGGGGC
GTGGCGCTGGTGGCAAACCTGGACGCGCGCGCAGTGGTTGATCGCTATCGGTCTATGCCCGGTGCTGCTGGCGTTGAACC
TGCCAATCAGCCTCAGCGGACCACTGGCGAATCTGCTGGCGGTGCCTTGGGTCAGTCTCGTGGTGCTGCCGCCAGCGTTG
CTCGGCACCCTATTGCTGCCGGTGCCCTATGTCGGCGAAGGTCTGTTGTGGCTGGCCGGCGGTTTGGTCGACTGGTTGTT
CCGCGTTCTGGCGCTGATTGCCGGGCAATGGCCGGCATGGACGGCGCCGTCGATATCCGCTTGGGTCTGGGCGCTGGGCA
GTTTCGGTGCGGTGCTGTTGCTACTGCCGCGCGGTGTGCCGATGCGAGCGCTGGGCTGGCCGTTGCTGTTGATCCTGGTG
GTGCCACCGCGAGAGCGATTGAATGAAGGGCTGGCGGATGTCTGGCAACTGGATGTCGGGCAGGGCTTGGCGATTCTGGT
CAGAACCCGCCACCACACGCTGCTCTACGACGCCGGTCCGCGTTTCGGCGAATTCGATCTCGGTGCGCGGGTGGTGCTGC
CGGCCCTGCGCAAACTCGGCGTGGAGAATCTCGATCTGCTGCTGCTCAGTTACGCCGATGCCGATCACGCTGGCGGTGCG
CTGAGCATTGCGCGCGGCCTGAAAGTCGCCAGAACAATCAGCGGCGATCCGCCGGGTCTGCCCGCTGAATTGAACGCGCA
AGCCTGCGAAAGCGGCCAGCAATGGCAATGGGATGGCGTGCGATTCCAACTCTGGCAATGGTCAGACGCCGATGACAGCA
ATCAGCGTTCCTGCGTCTTGCAGATCGAAGCGAATGGCGAGCGGTTGCTGCTGACCGGTGACATCGACACCCATGCCGAA
CGTGTCCTGCTCGACAGTCCCCTGGCAGTGCCGACGCATTGGCTGCAATCGCCGCACCACGGCAGCCGAAGTTCTTCGTC
GATGGCCTTGCTCAAGGTTTTGCAACCAGAAAGCGTGCTCATCTCCCGTGGCCATGGCAATTCGTTTGGCCATCCGCACC
CAACGGTTCTTGCCCGTTACCGCAAGCAACGCCTGCGGATTTACGACAGCGCTGAACACGGCGCCATTCATCTGCAACTG
GGCACCTTCAGCCCGCCCGTTATGATGCGTCAACAGCGGCGCTTCTGGCGCGATCCGCCTGCGCCTGCCCGCTGA

Domains

Predicted by InterproScan.

• Competence protein (Competence) (198-464)

• Metallo-beta-lactamase superfamily (Lactamase_B) (498-685)

• Domain of unknown function (DUF4131) (DUF4131) (24-167)

3D structure

• AlphaFold DB: A0A448DZX0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comA	Pseudomonas stutzeri DSM 10701	60.779	96.64	0.587
comA	Ralstonia pseudosolanacearum GMI1000	35.705	100	0.364