Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EL257_RS11970 Genome accession   NZ_LR134318
Coordinates   2652122..2653228 (+) Length   368 a.a.
NCBI ID   WP_126362793.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain NCTC9428     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2647122..2658228
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL257_RS11955 (NCTC9428_02388) - 2648062..2648997 (+) 936 WP_126362789.1 sugar kinase -
  EL257_RS11960 (NCTC9428_02389) - 2649126..2649401 (-) 276 WP_007912322.1 peptidylprolyl isomerase -
  EL257_RS11965 (NCTC9428_02390) - 2649450..2651990 (-) 2541 WP_126362791.1 PAS domain S-box protein -
  EL257_RS11970 (NCTC9428_02391) pilU 2652122..2653228 (+) 1107 WP_126362793.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EL257_RS11975 (NCTC9428_02392) - 2653235..2655190 (-) 1956 WP_126362795.1 acetoacetate--CoA ligase -
  EL257_RS11980 (NCTC9428_02393) hbdH 2655335..2656108 (-) 774 WP_126362797.1 3-hydroxybutyrate dehydrogenase -
  EL257_RS11985 (NCTC9428_02394) - 2656117..2657508 (-) 1392 WP_126362799.1 GntP family permease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 40993.06 Da        Isoelectric Point: 7.2355

>NTDB_id=1121319 EL257_RS11970 WP_126362793.1 2652122..2653228(+) (pilU) [Pseudomonas fluorescens strain NCTC9428]
MEIDALLTRLASQHGSDLYLSTGAPPSARFEGVLTPLSEQPFKPGEIAAIAHSLMDAEQRQTFDRDLEMNLAISRTGIGR
FRVNVFKQRNDVSIVIRNVKLDIPRFEDLKLPAILLETVMLKQGLILFVGATGSGKSTSLAALIDHRNRHSGGHIITIED
PIEYIHRHRRSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALSFAETGHLVLSTLHANNANQALERI
INMFAEERRPQLLLALGNHLKGFVSQRLVRTLDGKRRAAVEVMLGTPTIGDLIRRNELGELKGIMEKSAELGMQTFDTAL
HKLVVEGAISEEEALRHADSVNNLRLRLKLHADTTPVPPTSNEWGLMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=1121319 EL257_RS11970 WP_126362793.1 2652122..2653228(+) (pilU) [Pseudomonas fluorescens strain NCTC9428]
ATGGAAATCGATGCGCTGTTAACACGCCTGGCCAGTCAACATGGCTCGGACCTGTATCTGTCGACCGGAGCGCCGCCGAG
TGCGCGCTTCGAAGGGGTGCTGACACCCTTGAGTGAACAACCCTTCAAGCCCGGCGAGATTGCAGCCATCGCCCATTCTC
TGATGGACGCCGAACAACGCCAGACGTTCGATCGCGATCTGGAAATGAATCTGGCGATCTCACGCACCGGCATCGGACGT
TTTCGGGTCAATGTGTTTAAACAGCGTAATGACGTGTCAATCGTCATCCGCAACGTCAAACTCGACATCCCGCGCTTCGA
AGATCTCAAGCTGCCAGCGATCCTGCTTGAAACCGTGATGCTCAAGCAAGGGTTGATCCTGTTCGTCGGCGCCACCGGTT
CCGGCAAGTCCACCTCGCTGGCAGCGCTGATCGATCACCGAAACCGCCACAGCGGCGGGCATATCATTACCATTGAAGAT
CCGATCGAATACATCCACCGCCATCGGCGCTCGATCATCAATCAGCGTGAGGTCGGTGTCGACACGCGTAGTTTCCACGC
AGCGTTGAAAAATACATTGCGCCAGGCCCCGGACGTGGTGCTGATCGGGGAAATCCGCGACCGTGAAACCATGGAACACG
CGTTGTCCTTTGCCGAAACCGGACATCTGGTGTTGTCGACCTTGCACGCCAACAATGCCAACCAGGCTCTGGAGCGGATC
ATCAATATGTTTGCCGAAGAGCGCCGACCGCAGCTGTTGCTTGCGCTGGGTAACCACCTCAAAGGCTTTGTTTCACAGCG
CCTGGTGCGCACGCTTGACGGTAAACGGCGCGCCGCCGTCGAGGTGATGCTTGGCACGCCGACGATCGGCGATCTGATAC
GGCGCAACGAGTTGGGCGAGCTCAAGGGCATCATGGAAAAGTCCGCGGAGCTCGGCATGCAGACCTTCGATACGGCGCTG
CACAAGTTGGTGGTGGAAGGCGCGATCAGTGAAGAAGAAGCGCTCAGGCACGCCGACTCGGTGAACAATTTGCGCCTGCG
TTTAAAACTGCACGCGGACACGACGCCAGTGCCGCCCACCTCGAACGAGTGGGGCTTGATGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(57-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

55.041

99.728

0.549
  pilU Acinetobacter baylyi ADP1

54.261

95.652

0.519
  pilU Vibrio cholerae strain A1552

52.857

95.109

0.503
  pilT Pseudomonas aeruginosa PAK

38.663

93.478

0.361

Multiple sequence alignment