Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   EL121_RS02460 Genome accession   NZ_LR134310
Coordinates   544064..545362 (-) Length   432 a.a.
NCBI ID   WP_039197441.1    Uniprot ID   -
Organism   Actinobacillus equuli strain NCTC8529     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 546301..559822 544064..545362 flank 939


Gene organization within MGE regions


Location: 544064..559822
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL121_RS02460 (NCTC8529_00496) comE 544064..545362 (-) 1299 WP_039197441.1 type IV pilus secretin PilQ Machinery gene
  EL121_RS02465 (NCTC8529_00497) - 545374..545778 (-) 405 WP_039197443.1 hypothetical protein -
  EL121_RS02470 (NCTC8529_00498) - 545771..546298 (-) 528 WP_081978365.1 chromosome segregation protein -
  EL121_RS02475 (NCTC8529_00499) - 546301..546822 (-) 522 WP_039197445.1 hypothetical protein -
  EL121_RS02480 (NCTC8529_00500) - 546804..547490 (-) 687 WP_039197447.1 hypothetical protein -
  EL121_RS02485 (NCTC8529_00501) - 547629..550214 (+) 2586 WP_039197449.1 penicillin-binding protein 1A -
  EL121_RS02490 (NCTC8529_00502) aroK 550478..550999 (+) 522 WP_005623982.1 shikimate kinase AroK -
  EL121_RS02495 (NCTC8529_00503) aroB 551016..552104 (+) 1089 WP_039197450.1 3-dehydroquinate synthase -
  EL121_RS02500 (NCTC8529_00504) - 552108..552944 (+) 837 WP_039197452.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  EL121_RS02505 (NCTC8529_00505) - 553014..554372 (+) 1359 WP_039197454.1 sodium-dependent transporter -
  EL121_RS02510 (NCTC8529_00506) pgi 554501..556141 (-) 1641 WP_039197456.1 glucose-6-phosphate isomerase -
  EL121_RS02515 (NCTC8529_00507) ubiK 556180..556431 (-) 252 WP_039197458.1 ubiquinone biosynthesis accessory factor UbiK -
  EL121_RS02540 (NCTC8529_00512) aroE 557147..557971 (-) 825 WP_039197461.1 shikimate dehydrogenase -
  EL121_RS02545 (NCTC8529_00513) - 557977..558531 (-) 555 WP_039197463.1 Sua5/YciO/YrdC/YwlC family protein -
  EL121_RS02550 (NCTC8529_00514) - 558524..559057 (-) 534 WP_039197464.1 type I DNA topoisomerase -
  EL121_RS02555 (NCTC8529_00515) yqfB 559057..559362 (-) 306 WP_039197466.1 N(4)-acetylcytidine aminohydrolase -
  EL121_RS02560 (NCTC8529_00516) recX 559355..559810 (-) 456 WP_197050945.1 recombination regulator RecX -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 47692.84 Da        Isoelectric Point: 9.4293

>NTDB_id=1121001 EL121_RS02460 WP_039197441.1 544064..545362(-) (comE) [Actinobacillus equuli strain NCTC8529]
MRRVILLLLLILTPVMANTFSISLKNAPTAEVLSYLAEEYGKNIVLSGNIETNTTLRIENSDFDSVLKSITRANKLSSAY
ENQIYFIGHKKDEKAAMPIAVNPDVLKPKLITKTIKLDYAKAAEVIESLTKGSGNFLSENGYLHFDDRSNSLIIKDSPES
MKNIVKLIRNLDKPTEQIAIEARIVTISSENLQELGVRWGMFSPTNGHHKVAGSLEANGLPNTNHLNVNFPVNNAASIAL
QVAKINGRVLDLELTALEQENDVEIIASPRLLTTNKKPASIKQGTEIPYVLYNRKDEVKNIEFKEAVLGLQVTPHISNDN
QILLDLVVTQNSPNSTSSTVHGLVTIDKQELNTQVFAKHGETIVLGGIFQHLTAKGEDRVPILGSIPVIKKLFSHSSDRI
SKRELVIFVTPYIVKSGKQQISSHSSQKLPPK

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=1121001 EL121_RS02460 WP_039197441.1 544064..545362(-) (comE) [Actinobacillus equuli strain NCTC8529]
ATGCGAAGAGTGATTTTGTTGCTCCTTTTGATATTAACGCCGGTTATGGCAAATACTTTTTCTATCTCGCTGAAAAATGC
GCCGACAGCGGAAGTTTTAAGTTATTTAGCCGAAGAATACGGCAAAAATATTGTGTTAAGCGGCAATATTGAAACAAATA
CTACATTAAGAATTGAAAATAGCGATTTTGATAGCGTGTTAAAAAGTATTACTCGAGCAAATAAGCTCAGCAGTGCGTAT
GAAAACCAGATCTATTTTATTGGACACAAAAAAGATGAGAAAGCGGCTATGCCAATAGCTGTAAATCCGGATGTATTGAA
ACCTAAGTTGATTACTAAGACAATAAAATTAGATTATGCGAAGGCAGCGGAAGTGATTGAATCTTTAACCAAAGGTAGTG
GTAATTTTTTATCGGAAAACGGCTATCTACATTTTGATGATCGTAGTAATAGTTTGATAATTAAAGATAGCCCAGAATCG
ATGAAAAATATCGTGAAATTAATTAGAAATCTGGATAAACCGACTGAGCAGATTGCGATTGAAGCAAGGATAGTCACAAT
AAGTAGTGAAAATTTGCAAGAGCTTGGGGTACGCTGGGGAATGTTTTCTCCGACAAACGGACATCATAAAGTCGCTGGTT
CGCTTGAAGCGAATGGACTACCGAATACTAACCATTTAAACGTAAATTTTCCGGTAAATAATGCCGCTTCTATTGCGCTA
CAAGTGGCAAAAATTAATGGACGAGTGCTTGATTTGGAATTAACCGCTTTAGAGCAAGAAAATGATGTGGAAATTATTGC
CAGCCCTCGTTTACTGACCACTAATAAGAAACCGGCGAGTATTAAGCAAGGGACTGAAATTCCGTATGTGCTTTATAACC
GTAAAGATGAAGTGAAAAATATCGAATTTAAAGAAGCCGTTTTAGGGCTACAGGTCACGCCGCATATTTCAAACGATAAT
CAAATTTTGCTTGATTTGGTGGTGACACAAAATTCGCCGAATTCAACCAGTTCGACAGTTCATGGTTTAGTAACGATTGA
TAAACAGGAATTAAATACGCAAGTATTCGCTAAGCATGGTGAAACTATTGTGCTAGGCGGTATTTTTCAGCATTTAACCG
CAAAAGGTGAGGACAGAGTGCCGATTTTAGGTTCAATTCCGGTCATTAAAAAGTTATTTAGCCATTCCAGTGATAGGATC
AGTAAGCGCGAATTAGTTATTTTCGTTACACCTTATATTGTTAAAAGTGGAAAACAGCAAATTTCCTCGCATTCTTCACA
GAAATTACCGCCAAAATAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(112-177)

Bacterial type II and III secretion system protein (Secretin)

(256-415)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

67.146

96.528

0.648
  comE Haemophilus influenzae 86-028NP

54.654

96.991

0.53
  comE Haemophilus influenzae Rd KW20

55.831

93.287

0.521
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

43.855

96.065

0.421
  pilQ Vibrio cholerae strain A1552

43.855

96.065

0.421
  pilQ Vibrio campbellii strain DS40M4

41.706

97.685

0.407

Multiple sequence alignment