Detailed information NTG1120988

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: EL344_RS30150
• Genome accession: NZ_LR134309
• Coordinates: 6240016..6241080 (-)
• Length: 354 a.a.
• NCBI ID: WP_003110888.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain NCTC12903
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6235016..6246080

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL344_RS30125 (NCTC12903_05870)	aroK	6235551..6236069 (-)	519	WP_003095833.1	shikimate kinase AroK	-
EL344_RS30130 (NCTC12903_05871)	pilQ	6236081..6238225 (-)	2145	WP_003103267.1	type 4a pilus secretin PilQ	Machinery gene
EL344_RS30135 (NCTC12903_05872)	pilP	6238279..6238803 (-)	525	WP_003095836.1	type 4a pilus biogenesis lipoprotein PilP	-
EL344_RS30140 (NCTC12903_05873)	pilO	6238800..6239423 (-)	624	WP_003103268.1	type 4a pilus biogenesis protein PilO	-
EL344_RS30145 (NCTC12903_05874)	pilN	6239420..6240016 (-)	597	WP_003095839.1	type 4a pilus biogenesis protein PilN	-
EL344_RS30150 (NCTC12903_05875)	pilM	6240016..6241080 (-)	1065	WP_003110888.1	type IV pilus assembly protein PilM	Machinery gene
EL344_RS30155 (NCTC12903_05876)	-	6241265..6243736 (+)	2472	WP_003095841.1	penicillin-binding protein 1A	-
EL344_RS30160 (NCTC12903_05877)	-	6243841..6245109 (-)	1269	WP_003103278.1	malic enzyme	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37983.48 Da
• Isoelectric Point: 4.5405

>NTDB_id=1120988 EL344_RS30150 WP_003110888.1 6240016..6241080(-) (pilM) [Pseudomonas aeruginosa strain NCTC12903]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=1120988 EL344_RS30150 WP_003110888.1 6240016..6241080(-) (pilM) [Pseudomonas aeruginosa strain NCTC12903]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGGCTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAAAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAGGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.899	100	0.562
comM	Acinetobacter nosocomialis M2	55.899	100	0.562
comM	Acinetobacter baylyi ADP1	51.977	100	0.52
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466