Detailed information NTG1120946

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: EL295_RS24910
• Genome accession: NZ_LR134308
• Coordinates: 5087351..5088499 (-)
• Length: 382 a.a.
• NCBI ID: WP_126453365.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain NCTC11445
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5082351..5093499

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL295_RS24890 (NCTC11445_04869)	-	5083089..5084273 (-)	1185	WP_003100953.1	cystathionine gamma-synthase	-
EL295_RS24895 (NCTC11445_04870)	-	5084270..5085643 (-)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
EL295_RS24900 (NCTC11445_04871)	-	5085886..5086290 (-)	405	WP_003084560.1	NINE protein	-
EL295_RS24905 (NCTC11445_04872)	czcD	5086444..5087343 (+)	900	WP_126453362.1	CDF family zinc transporter CzcD	-
EL295_RS24910 (NCTC11445_04873)	pilU	5087351..5088499 (-)	1149	WP_126453365.1	type IV pilus ATPase PilU	Machinery gene
EL295_RS24915 (NCTC11445_04874)	pilT	5088677..5089711 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
EL295_RS24920 (NCTC11445_04875)	-	5089926..5090618 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
EL295_RS24925 (NCTC11445_04876)	proC	5090630..5091451 (+)	822	WP_003100962.1	pyrroline-5-carboxylate reductase	-
EL295_RS24930 (NCTC11445_04877)	-	5091462..5092055 (+)	594	WP_003084544.1	YggT family protein	-
EL295_RS24935	-	5092052..5092348 (+)	297	WP_031628073.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42474.77 Da
• Isoelectric Point: 6.9321

>NTDB_id=1120946 EL295_RS24910 WP_126453365.1 5087351..5088499(-) (pilU) [Pseudomonas aeruginosa strain NCTC11445]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDAGHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1120946 EL295_RS24910 WP_126453365.1 5087351..5088499(-) (pilU) [Pseudomonas aeruginosa strain NCTC11445]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACTTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGGCCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	59.497	93.717	0.558
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.899	93.717	0.393