Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   EL104_RS04585 Genome accession   NZ_LR134298
Coordinates   933624..934958 (+) Length   444 a.a.
NCBI ID   WP_074865595.1    Uniprot ID   -
Organism   Pasteurella multocida subsp. gallicida strain NCTC10204     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 928624..939958
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL104_RS04560 (NCTC10204_00922) - 928700..931261 (-) 2562 WP_016534629.1 penicillin-binding protein 1A -
  EL104_RS04565 (NCTC10204_00923) - 931397..932170 (+) 774 WP_014668624.1 pilus assembly protein PilM -
  EL104_RS04570 (NCTC10204_00924) - 932210..932725 (+) 516 WP_005751904.1 hypothetical protein -
  EL104_RS04575 (NCTC10204_00925) - 932725..933249 (+) 525 WP_005754754.1 hypothetical protein -
  EL104_RS04580 (NCTC10204_00926) - 933252..933614 (+) 363 WP_074865598.1 pilus assembly protein PilP -
  EL104_RS04585 (NCTC10204_00927) comE 933624..934958 (+) 1335 WP_074865595.1 type IV pilus secretin PilQ Machinery gene
  EL104_RS04590 (NCTC10204_00928) aroK 935141..935668 (+) 528 WP_005717623.1 shikimate kinase AroK -
  EL104_RS04595 (NCTC10204_00929) aroB 935685..936773 (+) 1089 WP_014668627.1 3-dehydroquinate synthase -
  EL104_RS04600 (NCTC10204_00930) - 936777..937682 (+) 906 WP_005723696.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  EL104_RS04605 (NCTC10204_00931) - 937729..938034 (-) 306 WP_005723691.1 DUF721 domain-containing protein -
  EL104_RS04610 (NCTC10204_00932) secM 938127..938438 (+) 312 WP_005723689.1 secA translation cis-regulator SecM -

Sequence

Protein


Download         Length: 444 a.a.        Molecular weight: 49300.69 Da        Isoelectric Point: 7.6664

>NTDB_id=1120691 EL104_RS04585 WP_074865595.1 933624..934958(+) (comE) [Pasteurella multocida subsp. gallicida strain NCTC10204]
MWRAFRKISFVYFLCGVAYVGSSQAQDAEHFYLRLKQAPLVEMLQYLALQQHQDLLIDDHLEGTLSLQMKKTTFEKCLQS
IAKMKQLELHQEGKSYYLTSPSSVAANDTHHPTSLMTSSIKLHFAKAEEVVKSLTSGQGSLLSVGGSLSFDERTNLLLIQ
DEPQSIQRIKALVAEMDKPIEQIAIEARIVTMTDESLQELGVRWGLFQATEQAHTIAGSLAANGFSNIENQLNVNFSTNS
APVGSIALQLAKINGRLLDLELTALEREKHIEIIASPRLLTTNKKSASIKQGTEIPYVMKRGKDKSESVEFREAVLGLDV
TPHISKDNTILLDLLITQNTLGAPVVYDKGEIVSIDKQEINTQVVAQDGETIVLGGVFHDTMTKGVNKVPLLGDLPLLKH
VFSQKTERHQKRELVIFVTPHIIKPSQGSPEQKTTRVKKSAKSR

Nucleotide


Download         Length: 1335 bp        

>NTDB_id=1120691 EL104_RS04585 WP_074865595.1 933624..934958(+) (comE) [Pasteurella multocida subsp. gallicida strain NCTC10204]
ATGTGGCGAGCATTCAGAAAAATATCTTTTGTGTACTTTTTATGTGGAGTTGCTTATGTTGGAAGTAGTCAAGCACAAGA
CGCAGAACATTTTTATTTACGTTTAAAACAAGCGCCTTTAGTCGAAATGTTACAGTATTTAGCATTGCAACAACATCAGG
ATTTGTTAATCGATGATCATTTAGAGGGCACATTATCATTACAGATGAAAAAGACAACCTTTGAGAAATGTTTACAGTCG
ATTGCAAAAATGAAACAACTTGAGTTACATCAAGAAGGAAAATCCTATTATTTAACTTCCCCTTCAAGTGTTGCAGCAAA
CGATACTCATCATCCTACGTCATTGATGACATCTTCAATAAAATTGCATTTTGCCAAAGCTGAAGAGGTGGTGAAATCTT
TAACTTCAGGGCAGGGAAGTTTACTTTCTGTCGGGGGGAGTTTGAGTTTTGATGAGCGGACTAATTTACTGCTGATTCAG
GATGAACCGCAATCAATACAGCGTATTAAAGCATTAGTAGCAGAAATGGATAAACCCATTGAACAAATTGCGATCGAAGC
TAGGATTGTGACGATGACAGACGAAAGTTTGCAGGAACTTGGTGTAAGATGGGGGCTATTTCAAGCAACAGAACAGGCAC
ATACTATTGCAGGGAGTTTAGCCGCGAACGGCTTTTCGAATATAGAAAACCAATTAAATGTGAATTTCTCGACCAATAGT
GCACCTGTTGGTTCCATCGCCTTACAGTTGGCGAAAATAAATGGTCGATTATTAGACTTGGAATTAACTGCCTTGGAGCG
AGAAAAGCATATTGAGATTATTGCGAGTCCTCGTTTATTAACAACGAATAAAAAAAGTGCCAGTATCAAACAAGGGACGG
AAATTCCTTATGTGATGAAGCGGGGAAAAGATAAAAGCGAATCGGTGGAATTTCGAGAAGCTGTATTGGGTTTAGATGTG
ACACCGCATATCTCAAAAGACAACACGATTTTATTAGATTTATTGATTACACAAAATACATTAGGTGCACCGGTAGTGTA
TGATAAAGGCGAAATTGTTTCGATCGATAAACAGGAAATCAATACTCAAGTCGTCGCTCAAGATGGTGAAACCATCGTTT
TAGGTGGGGTGTTTCATGATACGATGACAAAGGGAGTCAATAAAGTACCACTACTAGGGGATTTGCCTTTGCTTAAACAT
GTGTTTAGCCAGAAAACTGAACGTCATCAAAAGCGGGAATTAGTGATTTTTGTCACTCCTCATATTATCAAACCTAGCCA
AGGTTCGCCTGAACAAAAAACAACAAGAGTTAAAAAATCTGCAAAATCGAGGTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(119-182)

Bacterial type II and III secretion system protein (Secretin)

(264-424)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

61.806

97.297

0.601
  comE Haemophilus influenzae 86-028NP

61.111

97.297

0.595
  comE Glaesserella parasuis strain SC1401

49.192

97.523

0.48
  pilQ Vibrio campbellii strain DS40M4

39.524

94.595

0.374
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.806

92.793

0.369
  pilQ Vibrio cholerae strain A1552

39.806

92.793

0.369

Multiple sequence alignment