Detailed information NTG1120333

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: EL191_RS21875
• Genome accession: NZ_LR134290
• Coordinates: 4669108..4670253 (-)
• Length: 381 a.a.
• NCBI ID: WP_013717518.1
• Uniprot ID: -
• Organism: Ectopseudomonas mendocina strain NCTC10897
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4664108..4675253

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL191_RS21855 (NCTC10897_04388)	-	4665502..4666146 (-)	645	WP_041980228.1	DUF2076 domain-containing protein	-
EL191_RS21860 (NCTC10897_04389)	-	4666256..4666654 (-)	399	WP_013717515.1	TM2 domain-containing protein	-
EL191_RS21865 (NCTC10897_04390)	-	4666802..4668013 (+)	1212	WP_041980230.1	putative DNA modification/repair radical SAM protein	-
EL191_RS21870 (NCTC10897_04391)	-	4668013..4668873 (+)	861	WP_041980231.1	TIGR03915 family putative DNA repair protein	-
EL191_RS21875 (NCTC10897_04392)	pilU	4669108..4670253 (-)	1146	WP_013717518.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
EL191_RS21880 (NCTC10897_04393)	pilT	4670340..4671374 (-)	1035	WP_013717519.1	type IV pilus twitching motility protein PilT	Machinery gene
EL191_RS21885 (NCTC10897_04394)	-	4671450..4672142 (+)	693	WP_041980232.1	YggS family pyridoxal phosphate-dependent enzyme	-
EL191_RS21890 (NCTC10897_04395)	proC	4672160..4672978 (+)	819	WP_013717521.1	pyrroline-5-carboxylate reductase	-
EL191_RS21895 (NCTC10897_04396)	-	4672990..4673583 (+)	594	WP_013717522.1	YggT family protein	-
EL191_RS21900 (NCTC10897_04397)	-	4673729..4674019 (+)	291	WP_026042151.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42469.79 Da
• Isoelectric Point: 6.5931

>NTDB_id=1120333 EL191_RS21875 WP_013717518.1 4669108..4670253(-) (pilU) [Ectopseudomonas mendocina strain NCTC10897]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVTKNAMSPEMTRETVLGVMNEAQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETTIPTIDELKLPDILKKLSLTKRGLVLFVGATGTGKSTSLAAMIGYRNRNSSGHIISIED
PIEYIHQHQSCIVTQREVGIDTESFEVALKNTLRQAPDVIMIGEVRTRETMDHAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKGLMKRSNEQGMQTFDQA
LYNLYTQGEITYEDALLYADSANDLRLMIKLGSETDGDHLTSMAQGLALEVSEEDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=1120333 EL191_RS21875 WP_013717518.1 4669108..4670253(-) (pilU) [Ectopseudomonas mendocina strain NCTC10897]
ATGGAATTCGAAAAACTACTGCGCCTGATGGTCGAAAAAGGCGGTTCCGACCTGTTCATCACCGCTGGCGTGCCGCCGTC
GATGAAGGTCAACGGCAAGATCATGCCCGTGACCAAGAACGCCATGTCGCCGGAGATGACCCGCGAAACCGTGCTGGGTG
TGATGAACGAGGCGCAGCGCCGCGACTTCGCCGAGAACCACGAGTGCAACTTCGCCATCAGCGCCCGTGGCATCGGTCGC
TTCCGTGTCAGCGCCTTCTACCAGCGCAACCTGGTGGGCATGGTGCTGCGTCGTATCGAGACCACCATCCCGACCATCGA
TGAGCTGAAGCTGCCGGATATCCTCAAGAAACTGTCGCTGACCAAGCGCGGACTGGTGCTGTTCGTCGGTGCCACCGGTA
CCGGTAAATCCACCTCGCTGGCGGCGATGATCGGCTACCGTAACCGCAATAGCAGCGGCCACATCATCTCCATCGAAGAC
CCTATCGAATACATCCACCAGCACCAGAGCTGCATCGTCACGCAGCGCGAAGTGGGTATCGACACCGAGTCGTTCGAGGT
CGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATTGGCGAGGTACGGACCCGCGAGACCATGGATCACG
CCGTGGCCTTCGCCGAAACCGGGCACCTGTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGTTGGACCGCATC
ATCAACTTCTTCCCAGCCGACCGGCAGAACCAGGTGTGGATGGATCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCGGATGGCAAGGGACGTCGCGCGGTGATCGAGGTGCTGATCAATACCCCGCTGGCTGCCGATCTGA
TCCGCAAGGGCGAGGTGCACGAGCTCAAGGGCCTGATGAAGCGCTCCAACGAGCAGGGCATGCAGACCTTCGACCAGGCC
TTGTACAACCTCTATACCCAGGGTGAGATCACCTACGAAGATGCGCTGCTCTACGCCGATTCGGCCAACGATCTGCGTCT
GATGATCAAGCTCGGCTCTGAAACCGATGGCGATCACCTGACCAGCATGGCCCAGGGCCTGGCGCTGGAGGTCAGCGAGG
AAGATCCGGGGCGTCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	91.339	100	0.913
pilU	Acinetobacter baylyi ADP1	63.585	93.701	0.596
pilU	Vibrio cholerae strain A1552	58.286	91.864	0.535
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.452	88.189	0.383