Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EL191_RS13195 Genome accession   NZ_LR134290
Coordinates   2859068..2860288 (-) Length   406 a.a.
NCBI ID   WP_174447360.1    Uniprot ID   -
Organism   Ectopseudomonas mendocina strain NCTC10897     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2854068..2865288
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL191_RS13180 (NCTC10897_02656) - 2854912..2855811 (+) 900 WP_041978893.1 hydroxymethylglutaryl-CoA lyase -
  EL191_RS13185 (NCTC10897_02657) - 2856108..2858063 (+) 1956 WP_041978891.1 acetoacetate--CoA ligase -
  EL191_RS13190 (NCTC10897_02658) - 2858132..2858881 (+) 750 WP_041978889.1 ABC transporter substrate-binding protein -
  EL191_RS13195 (NCTC10897_02659) pilU 2859068..2860288 (-) 1221 WP_174447360.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EL191_RS13200 (NCTC10897_02660) - 2860438..2860716 (+) 279 WP_041978885.1 peptidylprolyl isomerase -
  EL191_RS13205 (NCTC10897_02661) - 2860724..2861635 (-) 912 WP_041978882.1 LysR family transcriptional regulator -
  EL191_RS13210 (NCTC10897_02662) - 2861704..2862183 (-) 480 WP_017362971.1 nuclear transport factor 2 family protein -
  EL191_RS13215 (NCTC10897_02663) - 2862193..2862783 (-) 591 WP_041978880.1 TetR/AcrR family transcriptional regulator -
  EL191_RS13220 (NCTC10897_02664) - 2862944..2863864 (-) 921 WP_041978878.1 LysR family transcriptional regulator -
  EL191_RS13225 (NCTC10897_02665) - 2864005..2864400 (+) 396 WP_041978876.1 RidA family protein -
  EL191_RS13230 (NCTC10897_02666) nfsB 2864625..2865275 (-) 651 WP_041978873.1 oxygen-insensitive NAD(P)H nitroreductase -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44741.05 Da        Isoelectric Point: 6.0784

>NTDB_id=1120325 EL191_RS13195 WP_174447360.1 2859068..2860288(-) (pilU) [Ectopseudomonas mendocina strain NCTC10897]
MDLHAMLKILSSQDGSDLYLSTGAPPCAKFNGVLKALSQEPMKAGDVAAIADSIMDSAQREEFERELEMNLAISLAGVGR
FRINIFKQRNEVSIVARNIKLDIPRFEDLKLPEVLLNTVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSGGHIITIED
PVEYVHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEDRRPQLLNDLANNLKAFVSQRLVKTVDGKRRAAVEVMLGTPTVRDLIKRNEFSELKEIMEKSKNLGMQTFDQAL
IDLVHEGVIDEEEAVKNADSANNVRLKLKLYRDNPANTRPAPAAAAPAAAPVAAPKAASAGDWGLELKLEDIEEEQPPED
PGRQGI

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=1120325 EL191_RS13195 WP_174447360.1 2859068..2860288(-) (pilU) [Ectopseudomonas mendocina strain NCTC10897]
ATGGATCTCCACGCAATGCTGAAAATCCTGTCCAGTCAGGATGGCTCCGATCTGTATCTGTCCACCGGCGCGCCGCCTTG
CGCCAAGTTCAACGGCGTGCTCAAGGCGCTGAGCCAGGAGCCGATGAAGGCGGGCGATGTGGCGGCCATTGCTGATTCGA
TCATGGATAGCGCGCAGCGCGAGGAGTTCGAGCGCGAACTGGAGATGAACCTGGCGATTTCCCTGGCTGGCGTAGGGCGT
TTTCGCATCAACATCTTCAAGCAGCGCAACGAGGTATCCATCGTCGCGCGTAACATCAAGCTGGACATCCCCCGCTTTGA
AGACCTCAAGCTGCCCGAGGTGCTGCTCAACACTGTGATGGAAAAGCGCGGCCTGGTGCTGTTCGTTGGCGGCACCGGCT
CGGGCAAGTCGACTTCTCTGGCGGCGCTGATCGACTACCGCAACCGCAACAGCGGCGGCCATATCATCACCATCGAAGAC
CCGGTGGAGTACGTGCACCGGCACAAGAAGTCGATCATCAACCAGCGTGAGGTGGGCGTGGACACGCGCAGCTTCCATGC
CGCGCTGAAGAACACCTTGCGTCAGGCGCCGGACGTGATCCTGATCGGTGAGATCCGCGACCGCGAAACCATGGAGCACG
CCCTGGCCTTTGCTGATACCGGGCACCTGGCGATCTCCACTTTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTTCCGGAAGACCGCCGCCCTCAGTTGCTCAACGACCTGGCTAACAACCTCAAGGCGTTCGTTTCCCAGCG
CCTGGTCAAGACCGTCGATGGCAAGCGCCGCGCCGCAGTGGAGGTGATGCTGGGCACACCGACGGTGCGCGACCTGATCA
AACGCAATGAATTCTCCGAGCTCAAGGAAATCATGGAGAAATCCAAGAACCTGGGCATGCAGACCTTCGATCAGGCGCTG
ATCGACCTGGTGCACGAAGGTGTGATCGATGAAGAAGAAGCGGTGAAGAACGCCGACTCGGCGAACAACGTACGTCTCAA
GCTCAAGCTCTATCGCGACAATCCGGCCAATACCAGGCCCGCACCCGCCGCGGCAGCGCCGGCAGCTGCGCCGGTCGCTG
CACCGAAGGCCGCCTCGGCAGGCGATTGGGGTCTGGAGCTCAAGCTGGAGGATATCGAGGAGGAGCAGCCGCCGGAGGAC
CCGGGACGGCAGGGGATTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.41

86.453

0.488
  pilU Acinetobacter baylyi ADP1

51.989

86.7

0.451
  pilU Vibrio cholerae strain A1552

51.841

86.946

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.796

96.552

0.384

Multiple sequence alignment