Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   EL191_RS02965 Genome accession   NZ_LR134290
Coordinates   638900..639964 (+) Length   354 a.a.
NCBI ID   WP_013713726.1    Uniprot ID   -
Organism   Ectopseudomonas mendocina strain NCTC10897     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 633900..644964
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL191_RS02955 (NCTC10897_00597) - 634863..636131 (+) 1269 WP_041976362.1 malic enzyme-like NAD(P)-binding protein -
  EL191_RS02960 (NCTC10897_00598) - 636307..638718 (-) 2412 WP_041976365.1 penicillin-binding protein 1A -
  EL191_RS02965 (NCTC10897_00599) pilM 638900..639964 (+) 1065 WP_013713726.1 pilus assembly protein PilM Machinery gene
  EL191_RS02970 (NCTC10897_00600) - 639964..640548 (+) 585 WP_017363399.1 PilN domain-containing protein -
  EL191_RS02975 (NCTC10897_00601) pilO 640535..641158 (+) 624 WP_017363398.1 type 4a pilus biogenesis protein PilO -
  EL191_RS02980 pilP 641155..641682 (+) 528 WP_017363397.1 type 4a pilus biogenesis lipoprotein PilP -
  EL191_RS02985 (NCTC10897_00602) pilQ 641737..643817 (+) 2081 Protein_599 type IV pilus secretin PilQ -
  EL191_RS02990 (NCTC10897_00603) aroK 643823..644341 (+) 519 WP_026042192.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37843.22 Da        Isoelectric Point: 4.4647

>NTDB_id=1120311 EL191_RS02965 WP_013713726.1 638900..639964(+) (pilM) [Ectopseudomonas mendocina strain NCTC10897]
MLGLFTKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALSRVLAKAKSGVKTAAV
AVAGSAVITKTIEMEAGLSEDDLENQLKIEADQYIPYPLEEVAIDFEVQGPAPRNPERVEVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAYSLLEAQLGGGHDELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGKQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKEAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTQTLVANP
FADMALSSKVNAGALASDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=1120311 EL191_RS02965 WP_013713726.1 638900..639964(+) (pilM) [Ectopseudomonas mendocina strain NCTC10897]
GTGCTAGGGCTCTTCACTAAGAAAGCGAATACGCTGCTGGGGATCGATATCAGCTCGACTTCGGTCAAGCTCCTCGAACT
GAGTCGCTCGGGAAGCCGCTACAAGGTAGAGGCATACGCAGTCGAGCCGCTTCCGCCAAACGCAGTGGTCGAGAAGAACA
TCGCCGAGCTGGAGGGGGTCGGTCAGGCGCTTTCTCGCGTACTGGCCAAGGCCAAGAGTGGTGTCAAGACAGCAGCCGTT
GCCGTTGCTGGTTCAGCCGTCATCACCAAGACCATCGAGATGGAAGCCGGGCTTTCCGAGGATGATCTGGAGAACCAGCT
GAAGATTGAGGCCGACCAGTACATTCCCTACCCGCTGGAAGAGGTCGCCATCGACTTCGAAGTGCAGGGGCCCGCGCCGC
GTAACCCCGAGCGAGTCGAAGTGCTGCTGGCTGCATGCCGCAAGGAAAACGTCGAGGTCCGCGAAGCGGCGCTGGCGCTT
GCCGGCTTGACCGCAAAAGTGGTGGACGTCGAGGCGTACGCGCTGGAGCGTGCCTACAGCCTGCTGGAGGCGCAGCTGGG
TGGTGGGCACGACGAACTGACGGTTGCGGTGGTGGATATCGGTGCGACCATGACCACGCTTAGCGTTCTGCACAACGGCC
GCACCATCTATACCCGTGAGCAGCTATTCGGCGGCAAGCAACTGACGGAAGAAATCCAGCGCCGTTATGGCCTGTCCGTC
GAGGAAGCTGGTCTTGCCAAGAAGCAAGGTGGTCTTCCAGATGACTACGACAGTGAAGTATTGCAACCGTTCAAAGAGGC
GGTCGTTCAGCAGGTCTCTCGCTCGCTGCAGTTCTTCTTCGCTGCTGGGCAGTTCAATGATGTTGACTACATCCTCCTGG
CTGGCGGCACTGCGTCCATCCCTGATCTTGATCGGCTGATCCAGCAGAAGATCGGTACGCAGACGCTAGTGGCCAATCCC
TTTGCCGACATGGCGCTCAGCAGCAAGGTCAACGCCGGTGCGTTGGCCAGTGATGCGCCGGCACTGATGATTGCCTGTGG
TCTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.78

100

0.568
  comM Acinetobacter nosocomialis M2

56.78

100

0.568
  comM Acinetobacter baylyi ADP1

53.672

100

0.537
  pilM Legionella pneumophila strain ERS1305867

44.915

100

0.449

Multiple sequence alignment