Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   EL087_RS01950 Genome accession   NZ_LR134288
Coordinates   387667..388968 (+) Length   433 a.a.
NCBI ID   WP_003028383.1    Uniprot ID   -
Organism   Streptococcus milleri strain NCTC11169     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 382667..393968
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL087_RS01935 (NCTC11169_00388) - 384026..385477 (+) 1452 WP_126406538.1 hypothetical protein -
  EL087_RS01940 (NCTC11169_00389) cysK 385946..386875 (-) 930 WP_003028385.1 cysteine synthase A -
  EL087_RS01945 (NCTC11169_00390) - 386975..387610 (-) 636 WP_106079174.1 YigZ family protein -
  EL087_RS01950 (NCTC11169_00391) comFA/cflA 387667..388968 (+) 1302 WP_003028383.1 DEAD/DEAH box helicase Machinery gene
  EL087_RS01955 (NCTC11169_00392) - 388965..389630 (+) 666 WP_126406541.1 ComF family protein -
  EL087_RS01960 (NCTC11169_00393) hpf 389709..390251 (+) 543 WP_003032381.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  EL087_RS01965 (NCTC11169_00394) rsmD 390439..390987 (+) 549 WP_025271932.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  EL087_RS01970 (NCTC11169_00395) coaD 390977..391474 (+) 498 WP_021001374.1 pantetheine-phosphate adenylyltransferase -
  EL087_RS01975 (NCTC11169_00396) sepM 391455..392510 (+) 1056 WP_126406544.1 SepM family pheromone-processing serine protease Regulator
  EL087_RS01980 (NCTC11169_00397) - 392650..393213 (+) 564 WP_003043206.1 YutD family protein -

Sequence

Protein


Download         Length: 433 a.a.        Molecular weight: 48876.73 Da        Isoelectric Point: 8.3812

>NTDB_id=1120247 EL087_RS01950 WP_003028383.1 387667..388968(+) (comFA/cflA) [Streptococcus milleri strain NCTC11169]
MTELQDCLGRIFTKSQLSPELQLQAQSLAGMVEEKGKLSCNRCGQAIDKEKQQLPIGAYYCRSCLILGRVRSDEELYYFP
QEEFPKANVLKWQGKLTEFQAKVSQGLVEAVTKRKDSLVHAVTGAGKTEMIYQVVAQVINQGGAVCLASPRIDVCLELYR
RLKVDFTCDISLLHGESEAYSRSPLVIATTHQLLKFYQAFDLLIVDEVDAFPYVDNPMLYHAVHQAVKVEGTKIFLTATS
TDELDKKVAKGELTRLSLPRRFHGNPLIVPQKIWLADFQKYLGQKKLVPKLRQFIQKQRKTSFPLLIFASEIKKGQELAE
ILQSTFPNEKVGFVASTTENRLEIVEKFRQKEITILVTTTILERGVTFPCVDVFVVEANHRLFSRSALVQIAGRVGRSMD
RPTGELIFFHDGATMAIEKAIKEIQEMNQEAGL

Nucleotide


Download         Length: 1302 bp        

>NTDB_id=1120247 EL087_RS01950 WP_003028383.1 387667..388968(+) (comFA/cflA) [Streptococcus milleri strain NCTC11169]
ATGACGGAATTACAAGATTGCTTAGGTCGTATTTTTACAAAAAGTCAATTGTCACCAGAATTGCAATTGCAAGCACAAAG
CTTAGCTGGAATGGTAGAAGAGAAAGGAAAGTTAAGCTGTAATCGCTGCGGACAAGCCATTGACAAAGAAAAACAGCAAC
TACCAATTGGTGCTTATTATTGTAGGTCTTGCTTGATCTTAGGAAGGGTTAGGAGTGATGAAGAACTATACTATTTTCCA
CAGGAAGAATTTCCCAAAGCGAATGTTTTGAAATGGCAAGGAAAGTTGACAGAATTTCAAGCCAAGGTTTCTCAAGGACT
TGTAGAGGCGGTTACCAAACGCAAAGATAGCTTGGTTCACGCAGTCACAGGAGCCGGAAAGACGGAAATGATCTATCAGG
TGGTGGCACAAGTCATCAATCAAGGTGGAGCCGTCTGCTTGGCTAGCCCCAGAATTGATGTCTGCTTAGAACTTTATCGC
AGACTGAAAGTAGATTTTACTTGCGATATTTCACTCCTGCACGGTGAATCAGAAGCATATTCCCGTAGTCCTCTCGTGAT
TGCTACAACGCATCAGCTTCTCAAATTTTATCAAGCCTTTGATCTTCTTATCGTTGATGAAGTAGATGCCTTTCCTTACG
TGGACAATCCGATGCTTTATCATGCGGTTCATCAGGCAGTTAAAGTAGAGGGAACGAAGATTTTCTTAACAGCAACTTCC
ACAGATGAGTTGGATAAAAAAGTGGCTAAAGGAGAATTAACTCGTTTGAGTCTACCTAGACGTTTTCATGGGAATCCTTT
GATTGTTCCTCAAAAAATTTGGTTGGCAGATTTTCAAAAATATCTTGGTCAAAAGAAGCTAGTTCCTAAGTTAAGACAGT
TTATTCAAAAGCAGAGAAAAACCAGCTTTCCACTTCTTATTTTTGCTTCTGAGATTAAAAAGGGGCAGGAATTAGCAGAG
ATTCTTCAAAGCACTTTTCCTAATGAAAAAGTTGGCTTTGTAGCCTCGACGACTGAAAATCGACTGGAAATTGTAGAGAA
ATTTCGTCAAAAAGAAATCACGATTTTGGTAACAACGACAATTCTAGAACGTGGTGTAACTTTTCCTTGTGTAGATGTTT
TTGTGGTGGAGGCTAACCACCGTTTGTTTAGTCGCAGCGCTCTGGTGCAAATCGCTGGACGCGTCGGTCGCAGTATGGAT
CGACCGACAGGCGAGTTAATCTTTTTTCATGACGGTGCGACTATGGCGATAGAAAAAGCGATTAAAGAAATTCAGGAGAT
GAATCAGGAGGCCGGTTTATGA

Domains

Predicted by InterproScan.

DEAD/DEAH box helicase (DEAD)

(108-245)

Helicase conserved C-terminal domain (Helicase_C)

(291-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

72.43

98.845

0.716
  comFA/cflA Streptococcus pneumoniae D39

72.43

98.845

0.716
  comFA/cflA Streptococcus pneumoniae R6

72.43

98.845

0.716
  comFA/cflA Streptococcus pneumoniae TIGR4

72.196

98.845

0.714
  comFA/cflA Streptococcus mitis NCTC 12261

72.066

98.383

0.709
  comFA/cflA Streptococcus mitis SK321

71.596

98.383

0.704
  comFA Lactococcus lactis subsp. cremoris KW2

55.779

91.917

0.513
  comFA Latilactobacillus sakei subsp. sakei 23K

40.647

100

0.406
  comFA Bacillus subtilis subsp. subtilis str. 168

39.409

93.764

0.37

Multiple sequence alignment