Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   EL079_RS05320 Genome accession   NZ_LR134283
Coordinates   1093635..1094936 (-) Length   433 a.a.
NCBI ID   WP_022524394.1    Uniprot ID   A0A3S4MUE3
Organism   Streptococcus anginosus strain NCTC10713     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1088635..1099936
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL079_RS05290 (NCTC10713_01104) - 1089391..1089954 (-) 564 WP_026248195.1 YutD family protein -
  EL079_RS05295 (NCTC10713_01105) sepM 1090094..1091149 (-) 1056 WP_003032358.1 SepM family pheromone-processing serine protease Regulator
  EL079_RS05300 (NCTC10713_01106) coaD 1091130..1091627 (-) 498 WP_003032432.1 pantetheine-phosphate adenylyltransferase -
  EL079_RS05305 (NCTC10713_01107) rsmD 1091617..1092165 (-) 549 WP_025271932.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  EL079_RS05310 (NCTC10713_01108) hpf 1092353..1092895 (-) 543 WP_003032381.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  EL079_RS05315 (NCTC10713_01109) comFC/cflB 1092973..1093638 (-) 666 WP_003032902.1 ComF family protein Machinery gene
  EL079_RS05320 (NCTC10713_01110) comFA/cflA 1093635..1094936 (-) 1302 WP_022524394.1 DEAD/DEAH box helicase Machinery gene
  EL079_RS05325 (NCTC10713_01111) - 1094993..1095628 (+) 636 WP_003032900.1 YigZ family protein -
  EL079_RS05330 (NCTC10713_01112) cysK 1095727..1096650 (+) 924 WP_003032904.1 cysteine synthase A -
  EL079_RS05335 (NCTC10713_01113) - 1096950..1097423 (-) 474 Protein_1047 DNA cytosine methyltransferase -
  EL079_RS05340 (NCTC10713_01114) - 1097602..1098429 (-) 828 WP_003030992.1 hypothetical protein -

Sequence

Protein


Download         Length: 433 a.a.        Molecular weight: 49110.09 Da        Isoelectric Point: 8.5998

>NTDB_id=1120032 EL079_RS05320 WP_022524394.1 1093635..1094936(-) (comFA/cflA) [Streptococcus anginosus strain NCTC10713]
MIELQDCLGRMFTKAQLPAELQLYAQTLPAMKEEKGKLCCNRCGQAVDKERHQLPIGAYYCRSCLILGRVRSDEDLYYFP
QEEFPKANVLKWQGKLTEFQAKVSQGLVEAVTKRKDSLVHAVTGAGKTEMIYQVVAQVINQGGAVCLASPRIDVCLELYR
RLKVDFTCDISLLRGESEAYSRSPLVIATTHQLLKFYQAFDLLIVDEVDAFPYVDNPMLYHAVHQAVKVEGTKIFLTATS
TDELDKKVAKGELTRLSLPRRFHGNPLIVPQKIWLADFQKYLGQKKLVTKLRQFIQKQRKTGFPLLIFASEIRRGQELAE
ILQSNFPNEKVGFVASTTENRLEIVEKFRQKEITILVTTTILERGVTFPCVDVFVVEANHRLFSRSALVQIAGRVGRSMD
RPTGELIFFHDGTTMAIEKAIKEIQEMNQEAGL

Nucleotide


Download         Length: 1302 bp        

>NTDB_id=1120032 EL079_RS05320 WP_022524394.1 1093635..1094936(-) (comFA/cflA) [Streptococcus anginosus strain NCTC10713]
ATGATAGAATTACAAGATTGCTTAGGCCGTATGTTTACAAAAGCACAACTACCAGCAGAATTGCAATTATATGCGCAAAC
TTTACCAGCAATGAAAGAAGAAAAAGGAAAATTATGTTGCAATCGTTGTGGGCAAGCAGTTGATAAAGAAAGACACCAAC
TGCCAATAGGTGCTTACTACTGTAGATCCTGCTTGATCTTAGGAAGGGTTAGAAGTGATGAAGATCTCTACTATTTTCCA
CAGGAAGAGTTTCCTAAAGCGAATGTCTTGAAATGGCAAGGAAAGTTGACAGAATTTCAAGCTAAGGTTTCTCAAGGACT
TGTAGAGGCGGTTACCAAACGCAAAGATAGCTTGGTTCACGCAGTCACAGGAGCCGGAAAGACGGAAATGATCTATCAGG
TGGTGGCACAAGTCATCAATCAAGGCGGAGCCGTCTGCTTAGCTAGCCCCAGAATTGATGTCTGCTTAGAACTTTATCGC
AGACTGAAAGTAGATTTTACCTGTGATATTTCACTCCTGCGCGGCGAATCAGAAGCATATTCCCGCAGTCCTCTCGTGAT
TGCCACCACACATCAGCTTCTCAAATTTTATCAAGCATTTGATCTTCTTATCGTTGATGAAGTAGATGCCTTTCCTTATG
TGGACAATCCGATGCTTTATCATGCAGTTCATCAGGCAGTCAAAGTAGAGGGGACGAAGATTTTCTTAACAGCAACTTCC
ACAGATGAGCTGGATAAAAAAGTGGCTAAAGGAGAATTAACTCGTTTGAGTCTACCCAGACGTTTTCATGGCAATCCTTT
GATTGTTCCGCAAAAAATTTGGTTGGCGGATTTTCAAAAATATCTTGGTCAAAAGAAGCTAGTTACCAAGCTAAGGCAGT
TTATTCAAAAGCAGAGAAAAACAGGATTTCCACTTCTTATTTTTGCTTCCGAGATCAGAAGAGGGCAGGAGCTGGCAGAG
ATTCTCCAAAGCAATTTTCCAAATGAAAAAGTTGGTTTTGTAGCTTCGACGACTGAAAATCGACTAGAAATTGTAGAGAA
GTTTCGTCAAAAAGAAATCACGATTTTGGTAACGACGACGATTTTGGAGCGTGGGGTGACCTTTCCTTGTGTAGATGTTT
TCGTGGTGGAAGCCAACCACCGTCTGTTTAGTCGCAGCGCTCTGGTACAAATCGCTGGACGCGTCGGTCGCAGTATGGAT
CGACCGACAGGCGAGTTAATCTTTTTTCATGATGGTACAACTATGGCGATAGAAAAAGCGATTAAAGAAATTCAGGAGAT
GAATCAGGAGGCCGGTTTATGA

Domains

Predicted by InterproScan.

DEAD/DEAH box helicase (DEAD)

(108-245)

Helicase conserved C-terminal domain (Helicase_C)

(290-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S4MUE3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

71.729

98.845

0.709
  comFA/cflA Streptococcus pneumoniae D39

71.729

98.845

0.709
  comFA/cflA Streptococcus pneumoniae R6

71.729

98.845

0.709
  comFA/cflA Streptococcus pneumoniae TIGR4

71.495

98.845

0.707
  comFA/cflA Streptococcus mitis NCTC 12261

71.596

98.383

0.704
  comFA/cflA Streptococcus mitis SK321

71.362

98.383

0.702
  comFA Lactococcus lactis subsp. cremoris KW2

56.03

91.917

0.515
  comFA Latilactobacillus sakei subsp. sakei 23K

41.763

99.538

0.416
  comFA Bacillus subtilis subsp. subtilis str. 168

40

92.379

0.37

Multiple sequence alignment