Detailed information NTG1119849

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: EL086_RS09665
• Genome accession: NZ_LR134274
• Coordinates: 2066772..2067209 (-)
• Length: 145 a.a.
• NCBI ID: WP_002887014.1
• Uniprot ID: A0A1V0GF29
• Organism: Streptococcus salivarius strain NCTC8618
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2061772..2072209

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL086_RS09630 (NCTC8618_04368)	-	2061815..2062477 (-)	663	WP_002887009.1	CPBP family intramembrane glutamic endopeptidase	-
EL086_RS09635 (NCTC8618_04369)	-	2062590..2063036 (-)	447	WP_037605837.1	hypothetical protein	-
EL086_RS09640 (NCTC8618_04370)	-	2063110..2063766 (-)	657	WP_002887011.1	CPBP family intramembrane glutamic endopeptidase	-
EL086_RS09645 (NCTC8618_04371)	-	2063778..2063975 (-)	198	WP_002885716.1	helix-turn-helix transcriptional regulator	-
EL086_RS09650 (NCTC8618_04372)	-	2064226..2065419 (-)	1194	WP_002885831.1	acetate kinase	-
EL086_RS09655 (NCTC8618_04373)	comYH	2065476..2066432 (-)	957	WP_038676858.1	class I SAM-dependent methyltransferase	Machinery gene
EL086_RS09660 (NCTC8618_04374)	comGG	2066477..2066794 (-)	318	WP_002887013.1	competence type IV pilus minor pilin ComGG	-
EL086_RS09665 (NCTC8618_04375)	comYF	2066772..2067209 (-)	438	WP_002887014.1	competence type IV pilus minor pilin ComGF	Machinery gene
EL086_RS09670 (NCTC8618_04376)	comGE	2067196..2067426 (-)	231	WP_002887015.1	competence type IV pilus minor pilin ComGE	-
EL086_RS09675 (NCTC8618_04377)	comYD	2067458..2067886 (-)	429	WP_254593949.1	competence type IV pilus minor pilin ComGD	Machinery gene
EL086_RS09680 (NCTC8618_04378)	comYC	2067846..2068160 (-)	315	WP_170314557.1	competence type IV pilus major pilin ComGC	Machinery gene
EL086_RS09685 (NCTC8618_04379)	comYB	2068169..2069269 (-)	1101	WP_126405090.1	competence type IV pilus assembly protein ComGB	Machinery gene
EL086_RS09690 (NCTC8618_04380)	comGA/cglA/cilD	2069151..2070092 (-)	942	WP_038676863.1	competence type IV pilus ATPase ComGA	Machinery gene
EL086_RS09695 (NCTC8618_04381)	-	2070172..2070534 (-)	363	WP_002887019.1	DUF1033 family protein	-

Sequence

Protein

• Length: 145 a.a.
• Molecular weight: 16155.57 Da
• Isoelectric Point: 8.4658

>NTDB_id=1119849 EL086_RS09665 WP_002887014.1 2066772..2067209(-) (comYF) [Streptococcus salivarius strain NCTC8618]
MSLNSKVRAFTLLECLVALLVIAGSVQVYQGLTTVLVSNVKQVKQQESQDWLLFVQQMEAELEGCQLVKVEGNKLYVKQD
NQDLAFGLSSATDFRKTNADGRGYQPMLFNVKASTISQNNQIVTIQVTLKNGLQRSFVYAFEKTG

Nucleotide

• Length: 438 bp

>NTDB_id=1119849 EL086_RS09665 WP_002887014.1 2066772..2067209(-) (comYF) [Streptococcus salivarius strain NCTC8618]
ATGTCTCTCAATAGCAAGGTTCGTGCTTTTACCCTCTTGGAATGTCTAGTGGCCTTGTTGGTGATTGCTGGTTCAGTTCA
AGTTTATCAAGGTCTGACCACAGTATTGGTGAGCAATGTTAAGCAGGTCAAGCAACAAGAAAGCCAAGACTGGCTTCTAT
TCGTTCAACAAATGGAGGCAGAATTAGAAGGTTGTCAGTTGGTAAAGGTAGAGGGGAACAAACTCTATGTCAAGCAGGAT
AATCAAGACTTAGCTTTTGGACTATCAAGTGCTACTGATTTTCGAAAAACAAATGCTGACGGTCGAGGCTACCAACCCAT
GCTTTTTAATGTAAAAGCTAGTACAATTAGCCAAAATAACCAGATAGTCACCATCCAAGTGACTCTTAAAAATGGGCTAC
AAAGGAGTTTTGTTTATGCTTTTGAAAAAACAGGTTAG

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (39-134)

3D structure

• AlphaFold DB: A0A1V0GF29

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	58.156	97.241	0.566
comYF	Streptococcus mutans UA159	57.447	97.241	0.559
comGF/cglF	Streptococcus pneumoniae Rx1	50	97.931	0.49
comGF/cglF	Streptococcus pneumoniae D39	50	97.931	0.49
comGF/cglF	Streptococcus pneumoniae R6	50	97.931	0.49
comGF/cglF	Streptococcus pneumoniae TIGR4	50	97.931	0.49
comGF/cglF	Streptococcus mitis SK321	47.222	99.31	0.469
comGF/cglF	Streptococcus mitis NCTC 12261	47.222	99.31	0.469
comGF	Lactococcus lactis subsp. cremoris KW2	45.324	95.862	0.434