Detailed information NTG1117216

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: EJN52_RS27625
• Genome accession: NZ_LR130535
• Coordinates: 5839956..5841020 (-)
• Length: 354 a.a.
• NCBI ID: WP_003110888.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa isolate paerg011
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5834956..5846020

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EJN52_RS27600 (BANRA_05357)	aroK	5835491..5836009 (-)	519	WP_003095833.1	shikimate kinase AroK	-
EJN52_RS27605 (BANRA_05358)	pilQ	5836021..5838165 (-)	2145	WP_014603377.1	type 4a pilus secretin PilQ	Machinery gene
EJN52_RS27610 (BANRA_05359)	pilP	5838219..5838743 (-)	525	WP_003095836.1	type 4a pilus biogenesis lipoprotein PilP	-
EJN52_RS27615 (BANRA_05360)	pilO	5838740..5839363 (-)	624	WP_003103268.1	type 4a pilus biogenesis protein PilO	-
EJN52_RS27620 (BANRA_05361)	pilN	5839360..5839956 (-)	597	WP_003095839.1	type 4a pilus biogenesis protein PilN	-
EJN52_RS27625 (BANRA_05362)	pilM	5839956..5841020 (-)	1065	WP_003110888.1	type IV pilus assembly protein PilM	Machinery gene
EJN52_RS27630 (BANRA_05363)	-	5841205..5843673 (+)	2469	WP_003114576.1	penicillin-binding protein 1A	-
EJN52_RS27635 (BANRA_05364)	-	5843778..5845046 (-)	1269	WP_004347579.1	malic enzyme-like NAD(P)-binding protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37983.48 Da
• Isoelectric Point: 4.5405

>NTDB_id=1117216 EJN52_RS27625 WP_003110888.1 5839956..5841020(-) (pilM) [Pseudomonas aeruginosa isolate paerg011]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=1117216 EJN52_RS27625 WP_003110888.1 5839956..5841020(-) (pilM) [Pseudomonas aeruginosa isolate paerg011]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGGCTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAAAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAAGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterProScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.899	100	0.562
comM	Acinetobacter nosocomialis M2	55.899	100	0.562
comM	Acinetobacter baylyi ADP1	51.977	100	0.52
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466