Detailed information NTG1117190

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: EJN52_RS02135
• Genome accession: NZ_LR130535
• Coordinates: 441276..442424 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa isolate paerg011
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 436276..447424

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EJN52_RS02110	-	437427..437723 (-)	297	WP_031628073.1	DUF167 domain-containing protein	-
EJN52_RS02115 (BANRA_00406)	-	437720..438313 (-)	594	WP_003084544.1	YggT family protein	-
EJN52_RS02120 (BANRA_00407)	proC	438324..439145 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
EJN52_RS02125 (BANRA_00408)	-	439157..439849 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
EJN52_RS02130 (BANRA_00409)	pilT	440064..441098 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
EJN52_RS02135 (BANRA_00410)	pilU	441276..442424 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
EJN52_RS02140 (BANRA_00411)	czcD	442432..443331 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
EJN52_RS02145 (BANRA_00412)	-	443485..443889 (+)	405	WP_003084560.1	NINE protein	-
EJN52_RS02150 (BANRA_00413)	-	444132..445505 (+)	1374	WP_003160915.1	pyridoxal-phosphate dependent enzyme	-
EJN52_RS02155 (BANRA_00414)	-	445502..446686 (+)	1185	WP_014602386.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=1117190 EJN52_RS02135 WP_003100959.1 441276..442424(+) (pilU) [Pseudomonas aeruginosa isolate paerg011]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1117190 EJN52_RS02135 WP_003100959.1 441276..442424(+) (pilU) [Pseudomonas aeruginosa isolate paerg011]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382