Detailed information NTG1117157

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: EJN62_RS02360
• Genome accession: NZ_LR130534
• Coordinates: 513841..514989 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa isolate paerg005
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 508841..519989

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EJN62_RS02335	-	509992..510288 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
EJN62_RS02340 (BANRA_00445)	-	510285..510878 (-)	594	WP_003084544.1	YggT family protein	-
EJN62_RS02345 (BANRA_00446)	proC	510889..511710 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
EJN62_RS02350 (BANRA_00447)	-	511722..512414 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
EJN62_RS02355 (BANRA_00448)	pilT	512629..513663 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
EJN62_RS02360 (BANRA_00449)	pilU	513841..514989 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
EJN62_RS02365 (BANRA_00450)	czcD	514997..515896 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
EJN62_RS02370 (BANRA_00451)	-	516051..516455 (+)	405	WP_003084560.1	NINE protein	-
EJN62_RS02375 (BANRA_00452)	-	516698..518071 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
EJN62_RS02380 (BANRA_00453)	-	518068..519252 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=1117157 EJN62_RS02360 WP_003110016.1 513841..514989(+) (pilU) [Pseudomonas aeruginosa isolate paerg005]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1117157 EJN62_RS02360 WP_003110016.1 513841..514989(+) (pilU) [Pseudomonas aeruginosa isolate paerg005]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCATG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGATCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382