Detailed information NTG1117089

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: EJM92_RS19740
• Genome accession: NZ_LR130531
• Coordinates: 4192883..4194031 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa isolate paerg004
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4187883..4199031

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EJM92_RS19715	-	4189034..4189330 (-)	297	WP_031628073.1	DUF167 domain-containing protein	-
EJM92_RS19720 (BANRA_03826)	-	4189327..4189920 (-)	594	WP_003084544.1	YggT family protein	-
EJM92_RS19725 (BANRA_03827)	proC	4189931..4190752 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
EJM92_RS19730 (BANRA_03828)	-	4190764..4191456 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
EJM92_RS19735 (BANRA_03829)	pilT	4191671..4192705 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
EJM92_RS19740 (BANRA_03830)	pilU	4192883..4194031 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
EJM92_RS19745 (BANRA_03831)	czcD	4194039..4194938 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
EJM92_RS19750 (BANRA_03832)	-	4195092..4195496 (+)	405	WP_003084560.1	NINE protein	-
EJM92_RS19755 (BANRA_03833)	-	4195739..4197112 (+)	1374	WP_003160915.1	pyridoxal-phosphate dependent enzyme	-
EJM92_RS19760 (BANRA_03834)	-	4197109..4198293 (+)	1185	WP_014602386.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=1117089 EJM92_RS19740 WP_003100959.1 4192883..4194031(+) (pilU) [Pseudomonas aeruginosa isolate paerg004]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1117089 EJM92_RS19740 WP_003100959.1 4192883..4194031(+) (pilU) [Pseudomonas aeruginosa isolate paerg004]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382