Detailed information NTG1117004

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: EJN10_RS02075
• Genome accession: NZ_LR130528
• Coordinates: 430966..432114 (+)
• Length: 382 a.a.
• NCBI ID: WP_124033924.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa isolate paerg000
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 425966..437114

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EJN10_RS02050	-	427117..427413 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
EJN10_RS02055 (BANRA_00395)	-	427410..428003 (-)	594	WP_003084544.1	YggT family protein	-
EJN10_RS02060 (BANRA_00396)	proC	428014..428835 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
EJN10_RS02065 (BANRA_00397)	-	428847..429539 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
EJN10_RS02070 (BANRA_00398)	pilT	429754..430788 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
EJN10_RS02075 (BANRA_00399)	pilU	430966..432114 (+)	1149	WP_124033924.1	type IV pilus ATPase PilU	Machinery gene
EJN10_RS02080 (BANRA_00400)	czcD	432122..433021 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
EJN10_RS02085 (BANRA_00401)	-	433175..433579 (+)	405	WP_125120823.1	NINE protein	-
EJN10_RS02090 (BANRA_00402)	-	433822..435195 (+)	1374	WP_124033925.1	pyridoxal-phosphate dependent enzyme	-
EJN10_RS02095 (BANRA_00403)	-	435192..436376 (+)	1185	WP_023114752.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42560.86 Da
• Isoelectric Point: 6.7792

>NTDB_id=1117004 EJN10_RS02075 WP_124033924.1 430966..432114(+) (pilU) [Pseudomonas aeruginosa isolate paerg000]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVVQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1117004 EJN10_RS02075 WP_124033924.1 430966..432114(+) (pilU) [Pseudomonas aeruginosa isolate paerg000]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTTGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGTCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60	91.623	0.55
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382