Detailed information NTG1116990

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: EJN50_RS17010
• Genome accession: NZ_LR130527
• Coordinates: 3555769..3556917 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa isolate paerg002
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3550769..3561917

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EJN50_RS16985	-	3551920..3552216 (-)	297	WP_031628073.1	DUF167 domain-containing protein	-
EJN50_RS16990 (BANRA_03304)	-	3552213..3552806 (-)	594	WP_003084544.1	YggT family protein	-
EJN50_RS16995 (BANRA_03305)	proC	3552817..3553638 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
EJN50_RS17000 (BANRA_03306)	-	3553650..3554342 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
EJN50_RS17005 (BANRA_03307)	pilT	3554557..3555591 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
EJN50_RS17010 (BANRA_03308)	pilU	3555769..3556917 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
EJN50_RS17015 (BANRA_03309)	czcD	3556925..3557824 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
EJN50_RS17020 (BANRA_03310)	-	3557978..3558382 (+)	405	WP_003084560.1	NINE protein	-
EJN50_RS17025 (BANRA_03311)	-	3558625..3559998 (+)	1374	WP_003160915.1	pyridoxal-phosphate dependent enzyme	-
EJN50_RS17030 (BANRA_03312)	-	3559995..3561179 (+)	1185	WP_014602386.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=1116990 EJN50_RS17010 WP_003100959.1 3555769..3556917(+) (pilU) [Pseudomonas aeruginosa isolate paerg002]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1116990 EJN50_RS17010 WP_003100959.1 3555769..3556917(+) (pilU) [Pseudomonas aeruginosa isolate paerg002]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382