Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiA   Type   Regulator
Locus tag   EW023_RS01475 Genome accession   NZ_LR130239
Coordinates   269606..271585 (+) Length   659 a.a.
NCBI ID   WP_023612402.1    Uniprot ID   A0A5S4TDS8
Organism   Streptococcus pyogenes strain PS003 isolate PS003     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 264606..276585
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW023_RS01455 sufU 264652..265131 (+) 480 WP_023612414.1 Fe-S cluster assembly sulfur transfer protein SufU -
  EW023_RS01460 sufB 265124..266542 (+) 1419 WP_002986015.1 Fe-S cluster assembly protein SufB -
  EW023_RS01465 - 266694..267875 (-) 1182 WP_023612404.1 D-alanyl-D-alanine carboxypeptidase family protein -
  EW023_RS01470 pbp3 268043..269275 (-) 1233 WP_023612400.1 D-alanyl-D-alanine carboxypeptidase PBP3 -
  EW023_RS01475 amiA 269606..271585 (+) 1980 WP_023612402.1 peptide ABC transporter substrate-binding protein Regulator
  EW023_RS01480 amiC 271650..273152 (+) 1503 WP_011017334.1 ABC transporter permease Regulator
  EW023_RS01485 amiD 273152..274078 (+) 927 WP_023612408.1 oligopeptide ABC transporter permease OppC Regulator
  EW023_RS01490 amiE 274087..275157 (+) 1071 WP_023612409.1 ABC transporter ATP-binding protein Regulator
  EW023_RS01495 amiF 275150..276073 (+) 924 WP_023612424.1 ABC transporter ATP-binding protein Regulator
  EW023_RS10015 - 276111..276200 (-) 90 WP_110002794.1 IS3 family transposase -
  EW023_RS01505 - 276221..276400 (-) 180 WP_023612403.1 hypothetical protein -

Sequence

Protein


Download         Length: 659 a.a.        Molecular weight: 72426.93 Da        Isoelectric Point: 5.1180

>NTDB_id=1116663 EW023_RS01475 WP_023612402.1 269606..271585(+) (amiA) [Streptococcus pyogenes strain PS003 isolate PS003]
MKKSKWLAAVSVAILSVSALAACGNKNASGGSEATKTYKYVFVNDPKSLDYILTNGGGTTDVITQMVDGLLENDEYGNLV
PSLAKDWKVSKDGLTYTYTLRDGVSWYTADGEEYAPVTAEDFVTGLKHAVDDKSDALYVVEDSIKNLKAYQNGEVDFKEV
GVKALDDKTVQYTLNKPESYWNSKTTYSVLFPVNAKFLKSKGKDFGTTDPSSILVNGAYFLSAFTSKSSMEFHKNENYWD
AKNVGIESVKLTYSDGSDPGSFYKNFDKGEFTVARLYPNDPTYKSAKKNYADNITYGMLTGDIRHLTWNLNRTSFKNTKK
DPAQQDAGKKALNNKDFRQAIQFAFDRASFQAQTAGQDAKTKALRNMLVPPTFVTIGESDFGSEVEKEMAKLGDEWKDVN
LADAQDGFYNPEKAKAEFAKAKEALTAEGVTFPVQLDYPVDQANAATVQEAQSFKQSVEASLGKENVIVNVLETETSTHE
AQGFYAETPEQQDYDIISSWWGPDYQDPRTYLDIMSPVGGGSVIQKLGIKAGQNKDVVAAAGLDTYQTLLDEAAAITDDN
DARYKAYAKAQAYLTDSAVDIPVVALGGTPRVTKAVPFSGAFAWAGAKGPASVYYYKGMKLQDKPVTAKQFEKAKEKWMK
AKAKSNAQYAEKLADHVEK

Nucleotide


Download         Length: 1980 bp        

>NTDB_id=1116663 EW023_RS01475 WP_023612402.1 269606..271585(+) (amiA) [Streptococcus pyogenes strain PS003 isolate PS003]
ATGAAGAAAAGTAAATGGTTGGCAGCTGTAAGTGTTGCGATCTTGTCAGTATCCGCTTTGGCAGCTTGTGGTAATAAAAA
TGCTTCAGGTGGCTCAGAAGCTACAAAAACCTACAAGTACGTTTTTGTTAACGATCCAAAATCATTGGATTATATTTTGA
CTAATGGCGGTGGAACGACTGATGTGATAACACAAATGGTTGATGGTCTTTTGGAAAACGATGAGTATGGTAATTTAGTA
CCATCACTTGCTAAAGATTGGAAGGTTTCAAAAGACGGTCTGACTTATACTTATACTCTTCGCGATGGTGTCTCTTGGTA
TACGGCTGATGGTGAAGAATATGCCCCAGTAACAGCAGAAGATTTTGTGACTGGTTTGAAGCACGCGGTTGACGATAAAT
CAGATGCTCTTTACGTTGTTGAAGATTCAATAAAAAACTTAAAGGCTTACCAAAATGGTGAAGTAGATTTTAAAGAAGTT
GGTGTCAAAGCCCTTGACGATAAAACTGTTCAGTATACTTTGAACAAGCCTGAAAGCTACTGGAATTCAAAAACAACTTA
TAGTGTGCTTTTCCCAGTTAATGCGAAATTTTTGAAGTCAAAAGGTAAAGATTTTGGTACAACCGATCCATCATCAATCC
TTGTTAATGGTGCTTACTTCTTGAGTGCCTTCACCTCAAAATCATCTATGGAATTCCATAAAAATGAAAACTACTGGGAT
GCTAAGAATGTTGGGATAGAATCTGTTAAATTGACTTACTCAGATGGTTCAGACCCAGGTTCGTTCTATAAGAACTTTGA
CAAGGGTGAGTTCACTGTCGCACGACTTTACCCAAATGACCCTACCTACAAATCAGCTAAGAAAAACTATGCTGATAATA
TTACTTACGGAATGTTGACTGGAGATATCCGTCATTTAACATGGAATTTGAACCGTACTTCTTTCAAAAACACTAAGAAA
GACCCTGCACAACAAGATGCCGGTAAGAAAGCTCTTAACAACAAGGATTTTCGTCAAGCTATTCAGTTTGCTTTTGACCG
AGCGTCATTCCAAGCCCAAACTGCAGGTCAAGATGCTAAAACAAAAGCCTTACGTAACATGCTTGTCCCACCAACATTTG
TGACCATTGGAGAAAGTGATTTTGGTTCAGAAGTTGAAAAGGAAATGGCAAAACTTGGTGATGAATGGAAAGACGTTAAC
TTAGCTGATGCTCAAGATGGTTTCTATAATCCTGAAAAAGCAAAAGCTGAGTTTGCAAAAGCCAAAGAAGCTTTGACAGC
TGAAGGTGTAACCTTCCCAGTTCAATTAGATTACCCTGTTGACCAAGCAAACGCAGCAACTGTTCAGGAAGCCCAGTCTT
TCAAACAATCTGTTGAAGCATCTCTTGGTAAAGAAAATGTCATTGTCAATGTTCTTGAAACAGAAACATCAACTCACGAA
GCCCAAGGCTTCTATGCTGAGACCCCAGAACAACAAGACTACGATATCATTTCATCATGGTGGGGACCAGACTACCAAGA
TCCACGGACCTACCTTGACATCATGAGTCCAGTAGGTGGTGGATCTGTTATCCAAAAACTTGGAATCAAAGCAGGTCAAA
ATAAGGATGTTGTGGCAGCTGCAGGCCTTGATACTTACCAAACTCTCCTTGATGAAGCAGCAGCAATTACAGACGACAAC
GATGCGCGCTATAAAGCTTACGCAAAAGCACAAGCCTACCTTACAGATAGTGCCGTAGATATTCCAGTTGTGGCATTGGG
TGGCACTCCTCGAGTTACTAAAGCCGTTCCATTTAGCGGTGCCTTTGCCTGGGCAGGTGCTAAAGGTCCTGCTTCTGTAT
ATTATTATAAAGGAATGAAACTTCAAGATAAACCTGTCACAGCAAAACAATTCGAAAAAGCAAAAGAAAAATGGATGAAA
GCAAAGGCTAAGTCAAATGCACAATATGCTGAGAAGTTAGCTGATCACGTTGAAAAATAA

Domains

Predicted by InterproScan.

Bacterial extracellular solute-binding proteins, family 5 Middle (SBP_bac_5)

(79-515)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5S4TDS8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiA Streptococcus salivarius strain HSISS4

50.83

100

0.511
  amiA3 Streptococcus thermophilus LMG 18311

50.075

100

0.504
  amiA3 Streptococcus thermophilus LMD-9

49.774

100

0.501

Multiple sequence alignment