Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   EW023_RS00670 Genome accession   NZ_LR130239
Coordinates   102530..103564 (+) Length   344 a.a.
NCBI ID   WP_229363300.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain PS003 isolate PS003     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 97530..108564
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW023_RS00660 - 101198..101563 (+) 366 WP_002986560.1 DUF1033 family protein -
  EW023_RS00665 comYA 101656..102594 (+) 939 WP_023612548.1 competence type IV pilus ATPase ComGA Machinery gene
  EW023_RS00670 comYB 102530..103564 (+) 1035 WP_229363300.1 competence type IV pilus assembly protein ComGB Machinery gene
  EW023_RS00675 comYC 103566..103892 (+) 327 WP_032465607.1 competence type IV pilus major pilin ComGC Machinery gene
  EW023_RS00680 comGD 103867..104295 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  EW023_RS00685 comGE 104252..104536 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  EW023_RS00690 comYF 104529..104963 (+) 435 WP_023612536.1 competence type IV pilus minor pilin ComGF Machinery gene
  EW023_RS00695 comGG 104947..105273 (+) 327 WP_002992739.1 competence type IV pilus minor pilin ComGG -
  EW023_RS00700 comYH 105371..106324 (+) 954 WP_002987790.1 class I SAM-dependent methyltransferase Machinery gene
  EW023_RS00705 - 106383..107579 (+) 1197 WP_023612537.1 acetate kinase -
  EW023_RS00710 - 107766..108074 (+) 309 WP_032465606.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39703.18 Da        Isoelectric Point: 9.7085

>NTDB_id=1116653 EW023_RS00670 WP_229363300.1 102530..103564(+) (comYB) [Streptococcus pyogenes strain PS003 isolate PS003]
MISFLRQDISIPSKLSLKKLSSKHQHKFIQLLANLLSTGFSFAEVIAFLKRSQLLQLDYVLKMEESLLKGQGLADMLSGL
GFSDAILTQISLADRHGNIETTLVAIQHYLNQMARIRRKTVEVITYPLILLLFLFVMMLGLRRYLVPQLETQNQITYFLN
HFPAFFIGFCSGLILLFGMVWLRWRSQNRLKLYSRLSRYPFLGKLLKQYLTSYYAREWGTLIGQGLDLMTILDIMAIEKS
SLMKELAEDIRMSLLEGQAFHIKVATYPFFKKELSLMIEYGEIKSKLGAELEIYAQESWEQFFSQLYQVTQLIQPAIFLV
VAVTIVMIYAAILLPIYQNMGGIF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1116653 EW023_RS00670 WP_229363300.1 102530..103564(+) (comYB) [Streptococcus pyogenes strain PS003 isolate PS003]
TTGATCAGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAGTAAACATCAGCATAA
ATTCATTCAATTACTAGCTAATCTTTTATCTACAGGATTTAGCTTCGCAGAGGTTATTGCTTTTTTAAAAAGAAGCCAGT
TGCTTCAGCTAGATTATGTTCTTAAAATGGAAGAGTCTTTATTAAAAGGACAAGGCTTAGCAGATATGTTGTCGGGTTTA
GGTTTTTCAGATGCTATTCTTACTCAAATTAGTTTAGCTGATAGACACGGTAATATTGAAACAACATTAGTAGCGATTCA
ACATTACTTGAACCAAATGGCAAGGATCAGACGAAAAACTGTTGAAGTTATCACCTATCCTCTTATTCTTTTACTTTTTC
TTTTTGTGATGATGCTAGGGTTGAGACGCTATTTAGTCCCTCAATTAGAAACTCAAAATCAGATAACTTACTTTCTTAAC
CATTTTCCTGCCTTCTTTATAGGTTTTTGCTCAGGTCTTATTTTGTTGTTCGGGATGGTATGGCTACGATGGCGATCTCA
AAACCGCTTGAAACTCTATAGTCGGCTAAGTCGTTATCCTTTTTTAGGTAAACTGTTAAAACAATACTTAACTAGTTATT
ATGCTAGAGAATGGGGTACGTTAATAGGTCAAGGTCTTGATTTGATGACTATTTTAGACATTATGGCCATTGAGAAATCC
TCACTAATGAAAGAATTAGCTGAGGACATCAGAATGAGTCTGCTTGAAGGGCAAGCCTTTCACATTAAAGTAGCTACCTA
CCCTTTTTTTAAAAAAGAATTAAGTTTAATGATTGAATACGGTGAAATCAAATCGAAACTGGGGGCTGAGTTGGAAATCT
ATGCCCAGGAAAGCTGGGAACAATTTTTTAGTCAGCTTTATCAAGTGACACAACTTATTCAACCAGCTATTTTTTTAGTA
GTTGCTGTGACAATCGTCATGATTTATGCGGCAATCCTATTACCAATTTACCAAAATATGGGAGGTATTTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(28-147)

Type II secretion system (T2SS), protein F (T2SSF)

(215-335)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

53.687

98.547

0.529
  comYB Streptococcus mutans UA159

53.687

98.547

0.529
  comYB Streptococcus gordonii str. Challis substr. CH1

50.588

98.837

0.5
  comGB/cglB Streptococcus mitis NCTC 12261

47.321

97.674

0.462
  comGB/cglB Streptococcus mitis SK321

47.447

96.802

0.459
  comGB/cglB Streptococcus pneumoniae Rx1

45.345

96.802

0.439
  comGB/cglB Streptococcus pneumoniae D39

45.345

96.802

0.439
  comGB/cglB Streptococcus pneumoniae R6

45.345

96.802

0.439
  comGB/cglB Streptococcus pneumoniae TIGR4

45.345

96.802

0.439
  comGB Lactococcus lactis subsp. cremoris KW2

42.462

94.477

0.401

Multiple sequence alignment