Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiA   Type   Regulator
Locus tag   EW021_RS08330 Genome accession   NZ_LR130236
Coordinates   1602758..1604728 (-) Length   656 a.a.
NCBI ID   WP_011285389.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain 5448 isolate 5448     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 1597758..1609728
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW021_RS09765 - 1598149..1598232 (+) 84 Protein_1552 IS3 family transposase -
  EW021_RS08310 amiF 1598270..1599193 (-) 924 WP_002986000.1 ABC transporter ATP-binding protein Regulator
  EW021_RS08315 amiE 1599186..1600256 (-) 1071 WP_010921893.1 ABC transporter ATP-binding protein Regulator
  EW021_RS08320 amiD 1600265..1601191 (-) 927 WP_010921892.1 oligopeptide ABC transporter permease OppC Regulator
  EW021_RS08325 amiC 1601191..1602693 (-) 1503 WP_010921891.1 ABC transporter permease Regulator
  EW021_RS08330 amiA 1602758..1604728 (-) 1971 WP_011285389.1 peptide ABC transporter substrate-binding protein Regulator
  EW021_RS08335 pbp3 1605059..1606291 (+) 1233 WP_010921890.1 D-alanyl-D-alanine carboxypeptidase PBP3 -
  EW021_RS08340 - 1606459..1607640 (+) 1182 WP_015055912.1 D-alanyl-D-alanine carboxypeptidase family protein -
  EW021_RS08345 sufB 1607792..1609210 (-) 1419 WP_010921889.1 Fe-S cluster assembly protein SufB -
  EW021_RS08350 sufU 1609203..1609682 (-) 480 WP_002986018.1 Fe-S cluster assembly sulfur transfer protein SufU -

Sequence

Protein


Download         Length: 656 a.a.        Molecular weight: 72102.60 Da        Isoelectric Point: 5.1964

>NTDB_id=1116527 EW021_RS08330 WP_011285389.1 1602758..1604728(-) (amiA) [Streptococcus pyogenes strain 5448 isolate 5448]
MKKSKWLAAVSVAILSVSALAACGNKNASGGSEATKTYKYVFVNDPKSLDYILTNGGGTTDVITQMVDGLLENDEYGNLV
PSLAKDWKVSKDGLTYTYTLRDGVSWYTADGEEYAPVTAEDFVTGLKHAVDDKSDALYVVEDSIKNLKAYQNGEVDFKEV
GVKALDDKTVQYTLNKPESYWNSKTTYSVLFPVNAKFLKSKGKDFGTTDPSSILVNGAYFLSAFTSKSSMEFHKNENYWD
AKNVGIESVKLTYSDGSDPGSFYKNFDKGEFSVARLYPNDPTYKSAKKNYADNITYGMLTGDIRHLTWNLNRTSFKNTKK
DPAQQDAGKKALNNKDFRQAIQFAFDRASFQAQTAGQDAKTKALRNMLVPPTFVTIGESDFGSEVEKEMAKLGDEWKDVN
LADAQDGFYNPEKAKAEFAKAKEALTAEGVTFPVQLDYPVDQANAATVQEAQSFKQSVEASLGKENVIVNVLETETSTHE
AQGFYAETPEQQDYDIISSWWGPDYQDPRTYLDIMSPVGGGSVIQKLGIKAGQNKDVVAAAGLDTYQTLLDEAAAITDDN
DARYKAYAKAQAYLTDNAVDIPVVALGGTPRVTKAVPFSGGFSWAGSKGPLAYKGMKLQDKPVTVKQYEKAKEKWMKAKA
KSNAKYAEKLADHVEK

Nucleotide


Download         Length: 1971 bp        

>NTDB_id=1116527 EW021_RS08330 WP_011285389.1 1602758..1604728(-) (amiA) [Streptococcus pyogenes strain 5448 isolate 5448]
ATGAAGAAAAGTAAATGGTTGGCAGCTGTAAGTGTTGCGATCTTGTCAGTATCCGCTTTGGCAGCTTGTGGTAATAAAAA
TGCTTCAGGTGGCTCAGAAGCTACAAAAACCTACAAGTACGTTTTTGTTAACGATCCAAAATCATTGGATTATATTTTGA
CTAATGGCGGTGGAACGACTGATGTGATAACACAAATGGTTGATGGTCTTTTGGAAAACGATGAGTATGGTAATTTAGTA
CCATCACTTGCTAAAGATTGGAAGGTTTCAAAAGACGGTCTGACTTATACTTATACTCTTCGCGATGGTGTCTCTTGGTA
TACGGCTGATGGTGAAGAATATGCCCCAGTAACAGCAGAAGATTTTGTGACTGGTTTGAAGCACGCGGTTGACGATAAAT
CAGATGCTCTTTACGTTGTTGAAGATTCAATAAAAAACTTAAAGGCTTACCAAAATGGTGAAGTAGATTTTAAAGAAGTT
GGTGTCAAAGCCCTTGACGATAAAACTGTTCAGTATACTTTGAACAAGCCTGAAAGCTACTGGAATTCAAAAACAACTTA
TAGTGTGCTTTTCCCAGTTAATGCGAAATTTTTGAAGTCAAAAGGTAAAGATTTTGGTACAACCGATCCATCATCAATCC
TTGTTAATGGTGCTTACTTCTTGAGCGCCTTCACCTCAAAATCATCTATGGAATTCCATAAAAATGAAAACTACTGGGAT
GCTAAGAATGTTGGGATAGAATCTGTTAAATTGACTTACTCAGATGGTTCAGACCCAGGTTCGTTCTACAAGAACTTTGA
CAAGGGTGAGTTCAGCGTTGCACGACTTTACCCAAATGACCCTACCTACAAATCAGCTAAGAAAAACTATGCTGATAACA
TTACTTACGGAATGTTGACTGGAGATATCCGTCATTTAACATGGAATTTGAACCGTACTTCTTTCAAAAACACTAAGAAA
GACCCTGCACAACAAGATGCCGGTAAGAAAGCTCTTAACAACAAGGATTTTCGTCAAGCTATTCAGTTTGCTTTTGACCG
AGCGTCATTCCAAGCACAAACTGCAGGTCAAGATGCCAAAACAAAAGCCTTACGTAACATGCTTGTCCCACCAACATTTG
TGACCATTGGAGAAAGTGATTTTGGTTCAGAAGTTGAAAAGGAAATGGCAAAACTTGGGGATGAATGGAAAGACGTTAAC
TTAGCTGATGCTCAAGATGGTTTCTATAATCCTGAAAAAGCAAAAGCTGAGTTTGCAAAAGCCAAAGAAGCTTTAACAGC
TGAAGGTGTAACCTTCCCAGTTCAATTAGATTACCCTGTTGACCAAGCAAACGCAGCAACTGTTCAGGAAGCCCAGTCTT
TCAAACAATCTGTTGAAGCATCTCTTGGTAAAGAGAATGTCATTGTCAATGTTCTTGAAACAGAAACATCAACTCACGAA
GCCCAAGGCTTCTATGCTGAGACCCCAGAACAACAAGACTACGATATCATTTCATCATGGTGGGGACCAGACTATCAAGA
TCCACGGACCTACCTTGACATCATGAGTCCAGTAGGTGGTGGATCTGTTATCCAAAAACTTGGAATCAAAGCAGGTCAAA
ATAAGGATGTTGTGGCAGCTGCAGGCCTTGATACCTACCAAACTCTTCTTGATGAAGCAGCAGCAATTACAGACGACAAC
GATGCGCGCTATAAAGCTTACGCAAAAGCACAAGCCTACCTTACAGATAATGCCGTAGATATTCCAGTTGTGGCATTGGG
TGGCACTCCACGAGTTACTAAAGCCGTTCCATTTAGCGGGGGCTTCTCTTGGGCAGGGTCTAAAGGTCCTCTAGCATATA
AAGGAATGAAACTTCAAGACAAACCTGTCACAGTAAAACAATACGAAAAAGCAAAAGAAAAATGGATGAAAGCAAAGGCT
AAGTCAAATGCAAAATATGCTGAGAAGTTAGCTGATCACGTTGAAAAATAA

Domains

Predicted by InterproScan.

Bacterial extracellular solute-binding proteins, family 5 Middle (SBP_bac_5)

(79-515)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiA Streptococcus salivarius strain HSISS4

51.135

100

0.515
  amiA3 Streptococcus thermophilus LMG 18311

49.849

100

0.503
  amiA3 Streptococcus thermophilus LMD-9

49.547

100

0.5

Multiple sequence alignment