Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   E0E08_RS08390 Genome accession   NZ_LR027873
Coordinates   1659833..1660330 (-) Length   165 a.a.
NCBI ID   WP_001803317.1    Uniprot ID   A0A2I7Y542
Organism   Staphylococcus aureus strain BPH2070 isolate BPH2070     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1654833..1665330
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0E08_RS08365 gcvPB 1654995..1656467 (-) 1473 WP_000202189.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  E0E08_RS08370 gcvPA 1656460..1657806 (-) 1347 WP_000019684.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  E0E08_RS08375 gcvT 1657826..1658917 (-) 1092 WP_000093351.1 glycine cleavage system aminomethyltransferase GcvT -
  E0E08_RS08380 - 1659076..1659600 (-) 525 WP_001015117.1 shikimate kinase -
  E0E08_RS08385 - 1659590..1659736 (-) 147 WP_001789879.1 hypothetical protein -
  E0E08_RS08390 comGF 1659833..1660330 (-) 498 WP_001803317.1 competence type IV pilus minor pilin ComGF Machinery gene
  E0E08_RS08395 comGE 1660248..1660547 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  E0E08_RS08400 comGD 1660534..1660980 (-) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  E0E08_RS08405 comGC 1660958..1661269 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  E0E08_RS08410 comGB 1661283..1661588 (-) 306 WP_332005405.1 type II secretion system F family protein Machinery gene
  E0E08_RS08415 - 1661574..1662746 (+) 1173 WP_000195429.1 IS256-like element IS256 family transposase -
  E0E08_RS08420 - 1662798..1663406 (-) 609 Protein_1582 type II secretion system F family protein -
  E0E08_RS08425 comGA 1663378..1664352 (-) 975 WP_000697228.1 competence type IV pilus ATPase ComGA Machinery gene
  E0E08_RS08430 - 1664404..1665027 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19212.92 Da        Isoelectric Point: 10.2192

>NTDB_id=1115872 E0E08_RS08390 WP_001803317.1 1659833..1660330(-) (comGF) [Staphylococcus aureus strain BPH2070 isolate BPH2070]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMFISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=1115872 E0E08_RS08390 WP_001803317.1 1659833..1660330(-) (comGF) [Staphylococcus aureus strain BPH2070 isolate BPH2070]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGTTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(67-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2I7Y542

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

99.394

100

0.994
  comGF Staphylococcus aureus N315

95.758

100

0.958

Multiple sequence alignment