Detailed information NTG1115715

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: TTHNP4_RS01400
• Genome accession: NZ_LR027517
• Coordinates: 239297..240430 (-)
• Length: 377 a.a.
• NCBI ID: WP_124104242.1
• Uniprot ID: A0A3P4AN88
• Organism: Thermus thermophilus isolate TTHNAR1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 234297..245430

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
TTHNP4_RS01380 (TTHN1_00275)	pilQ	234953..237226 (-)	2274	WP_124104239.1	secretin N-terminal domain-containing protein	Machinery gene
TTHNP4_RS14400 (TTHN1_00276)	pilW	237223..238113 (-)	891	WP_124104240.1	competence protein	Machinery gene
TTHNP4_RS01390 (TTHN1_00277)	pilO	238110..238691 (-)	582	WP_124104241.1	type 4a pilus biogenesis protein PilO	Machinery gene
TTHNP4_RS01395 (TTHN1_00278)	pilN	238681..239304 (-)	624	WP_011228637.1	competence protein	Machinery gene
TTHNP4_RS01400 (TTHN1_00279)	pilM	239297..240430 (-)	1134	WP_124104242.1	type IV pilus assembly protein PilM	Machinery gene
TTHNP4_RS01405 (TTHN1_00280)	-	240560..241564 (-)	1005	WP_124104243.1	homoisocitrate dehydrogenase	-
TTHNP4_RS01410 (TTHN1_00281)	-	241619..242110 (-)	492	WP_011173430.1	DUF5317 domain-containing protein	-
TTHNP4_RS01415 (TTHN1_00282)	-	242161..243561 (-)	1401	WP_124104244.1	HD-GYP domain-containing protein	-
TTHNP4_RS01435 (TTHN1_00285)	-	244345..244710 (+)	366	WP_172596880.1	hypothetical protein	-

Sequence

Protein

• Length: 377 a.a.
• Molecular weight: 41369.01 Da
• Isoelectric Point: 4.3773

>NTDB_id=1115715 TTHNP4_RS01400 WP_124104242.1 239297..240430(-) (pilM) [Thermus thermophilus isolate TTHNAR1]
MFKSLSQLFRPRVEALGLEIGASALKLVEVSGNPPALKALASRPTPPGLLVEGMVAEPAALAQEIKELLLEARTRKRYVV
TALSNLAVILRPIQVPKMPLKEMEEAVRWEAERYIPFPIDEVVLDFAPLTPLSEVQEGEQVQVMVAAARQEAVAGVLEAL
RGAGLVPVVLDVKPFAGLYPLEAKLAEDPDRVFLVLDIGAESTSLVLLRGDKPLAVRILTLSGKDFTEAIAQSFNLDLLT
AEEVKRTYGMATIPTEDEELLLDFDAERERYSPGRIYDAIRPVLVELTQELRRSLEFFRIQLEEAPPEVGFLLGGGSKLR
GLASLLTDTLGVNFEPVNPWEAVTVDPKRFEAEKLQEMGPEFAVALGLALRGVEPFD

Nucleotide

• Length: 1134 bp

>NTDB_id=1115715 TTHNP4_RS01400 WP_124104242.1 239297..240430(-) (pilM) [Thermus thermophilus isolate TTHNAR1]
GTGTTCAAAAGCCTTAGCCAGCTCTTCCGACCCAGGGTTGAGGCCCTGGGCTTGGAGATCGGGGCCTCCGCCCTGAAGCT
CGTGGAGGTGTCCGGGAACCCCCCTGCCCTCAAGGCCCTGGCCTCCCGCCCCACCCCGCCCGGCCTCCTGGTGGAAGGAA
TGGTGGCCGAGCCCGCCGCGTTGGCCCAGGAGATCAAGGAGCTTCTCCTCGAGGCCCGCACCCGCAAGCGCTACGTGGTC
ACCGCCCTCTCCAACCTGGCGGTCATCCTCCGCCCCATCCAGGTTCCCAAGATGCCCCTCAAGGAGATGGAGGAGGCGGT
GCGCTGGGAGGCGGAGCGCTACATCCCCTTCCCCATTGACGAGGTGGTCCTGGACTTCGCCCCCCTGACCCCCCTCTCCG
AGGTGCAGGAGGGGGAGCAGGTCCAGGTGATGGTGGCGGCGGCGCGGCAGGAGGCGGTGGCGGGGGTCCTCGAGGCCTTG
AGGGGGGCGGGGCTCGTCCCCGTGGTGCTGGACGTGAAGCCCTTCGCCGGGCTCTACCCCCTGGAGGCCAAGCTTGCGGA
GGACCCCGACCGGGTCTTCCTCGTTCTGGACATTGGGGCCGAGAGCACAAGCCTCGTCCTGCTCCGGGGCGATAAGCCTT
TGGCGGTGCGCATCCTGACCCTCTCGGGCAAGGACTTCACCGAGGCCATCGCGCAAAGTTTCAATCTGGACCTCCTTACC
GCCGAGGAGGTGAAGCGGACCTACGGGATGGCCACCATCCCCACCGAGGACGAGGAGCTCCTCCTGGACTTTGACGCCGA
GCGGGAGCGCTACAGCCCGGGGCGCATCTACGACGCCATCCGCCCGGTGCTGGTGGAGCTCACCCAGGAGCTCCGCCGTA
GCCTAGAGTTCTTCCGCATCCAGCTGGAAGAAGCCCCGCCCGAGGTGGGCTTCCTCCTGGGCGGGGGAAGCAAGTTGAGG
GGGCTTGCCTCCCTCCTCACCGACACCCTGGGGGTGAACTTCGAGCCCGTCAATCCCTGGGAGGCGGTAACGGTGGACCC
CAAGCGCTTTGAGGCGGAGAAGCTCCAGGAAATGGGGCCCGAGTTCGCCGTAGCCCTGGGCCTGGCCCTGAGGGGGGTGG
AGCCCTTTGATTAG

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (17-372)

3D structure

• AlphaFold DB: A0A3P4AN88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Thermus thermophilus HB27	96.021	100	0.96