Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   MIDG2331_RS04440 Genome accession   NZ_LN908249
Coordinates   989610..990056 (-) Length   148 a.a.
NCBI ID   WP_005597397.1    Uniprot ID   B0BPG5
Organism   Actinobacillus pleuropneumoniae serovar 8 isolate MIDG2331     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 984610..995056
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MIDG2331_RS04415 (MIDG2331_00904) yacG 985517..985705 (-) 189 WP_005597388.1 DNA gyrase inhibitor YacG -
  MIDG2331_RS04420 (MIDG2331_00905) coaE 985687..986325 (-) 639 WP_005601202.1 dephospho-CoA kinase -
  MIDG2331_RS04425 (MIDG2331_00906) - 986347..986985 (-) 639 WP_005604550.1 prepilin peptidase -
  MIDG2331_RS04430 (MIDG2331_00907) pilC 987012..988214 (-) 1203 WP_012263006.1 type II secretion system F family protein Machinery gene
  MIDG2331_RS04435 (MIDG2331_00908) pilB 988198..989592 (-) 1395 WP_005601209.1 GspE/PulE family protein Machinery gene
  MIDG2331_RS04440 (MIDG2331_00909) pilA 989610..990056 (-) 447 WP_005597397.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  MIDG2331_RS04445 (MIDG2331_00910) radA 990238..991617 (+) 1380 WP_005601211.1 DNA repair protein RadA Machinery gene
  MIDG2331_RS04450 (MIDG2331_00911) - 991661..991894 (-) 234 WP_005597399.1 YgjV family protein -
  MIDG2331_RS04455 (MIDG2331_00912) folA 992021..992509 (-) 489 WP_005597400.1 type 3 dihydrofolate reductase -
  MIDG2331_RS04460 (MIDG2331_00913) proB 992596..993687 (+) 1092 WP_005601213.1 glutamate 5-kinase -
  MIDG2331_RS04465 (MIDG2331_00914) - 993700..994476 (+) 777 WP_005597402.1 TSUP family transporter -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15980.38 Da        Isoelectric Point: 7.6257

>NTDB_id=1115302 MIDG2331_RS04440 WP_005597397.1 989610..990056(-) (pilA) [Actinobacillus pleuropneumoniae serovar 8 isolate MIDG2331]
MQKLSLIRPLTNAFTLIELMIVIAIIAILATVAIPSYNSYTQKAALSELLAASASYKTDVEICIYNTGDSKNCSGGQNGV
RKMTELRQAKYLNAITVEGGTITVTGKGNLQEYGYTMTPIHNGSTISWETKCKGEDLSLFPANFCASN

Nucleotide


Download         Length: 447 bp        

>NTDB_id=1115302 MIDG2331_RS04440 WP_005597397.1 989610..990056(-) (pilA) [Actinobacillus pleuropneumoniae serovar 8 isolate MIDG2331]
ATGCAAAAACTAAGTCTTATTCGACCGCTTACTAACGCGTTTACTTTAATTGAATTGATGATCGTGATTGCGATTATTGC
CATTTTAGCTACGGTTGCAATTCCGTCATATAACAGTTATACCCAAAAAGCGGCGCTTTCGGAGCTATTGGCGGCATCGG
CTTCTTATAAAACGGATGTCGAGATCTGCATATATAACACCGGAGATTCTAAAAACTGTAGCGGCGGTCAAAACGGTGTC
AGAAAAATGACGGAGCTTAGACAGGCTAAATATTTAAATGCCATTACGGTGGAAGGCGGAACGATTACGGTAACGGGGAA
AGGGAATCTACAGGAATACGGTTATACGATGACACCGATTCATAACGGTAGCACTATTTCTTGGGAAACGAAATGTAAAG
GGGAGGACTTAAGTTTATTTCCGGCAAATTTCTGCGCATCAAATTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(13-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B0BPG5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

68.919

100

0.689
  pilA Haemophilus influenzae 86-028NP

55.102

99.324

0.547
  pilA Haemophilus influenzae Rd KW20

53.061

99.324

0.527

Multiple sequence alignment