Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   MIDG2331_RS04430 Genome accession   NZ_LN908249
Coordinates   987012..988214 (-) Length   400 a.a.
NCBI ID   WP_012263006.1    Uniprot ID   B0BPG3
Organism   Actinobacillus pleuropneumoniae serovar 8 isolate MIDG2331     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 982012..993214
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MIDG2331_RS04405 (MIDG2331_00902) lptE 983812..984306 (+) 495 WP_005597385.1 LPS assembly lipoprotein LptE -
  MIDG2331_RS04410 (MIDG2331_00903) holA 984415..985440 (+) 1026 WP_012263005.1 DNA polymerase III subunit delta -
  MIDG2331_RS04415 (MIDG2331_00904) yacG 985517..985705 (-) 189 WP_005597388.1 DNA gyrase inhibitor YacG -
  MIDG2331_RS04420 (MIDG2331_00905) coaE 985687..986325 (-) 639 WP_005601202.1 dephospho-CoA kinase -
  MIDG2331_RS04425 (MIDG2331_00906) - 986347..986985 (-) 639 WP_005604550.1 prepilin peptidase -
  MIDG2331_RS04430 (MIDG2331_00907) pilC 987012..988214 (-) 1203 WP_012263006.1 type II secretion system F family protein Machinery gene
  MIDG2331_RS04435 (MIDG2331_00908) pilB 988198..989592 (-) 1395 WP_005601209.1 GspE/PulE family protein Machinery gene
  MIDG2331_RS04440 (MIDG2331_00909) pilA 989610..990056 (-) 447 WP_005597397.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  MIDG2331_RS04445 (MIDG2331_00910) radA 990238..991617 (+) 1380 WP_005601211.1 DNA repair protein RadA Machinery gene
  MIDG2331_RS04450 (MIDG2331_00911) - 991661..991894 (-) 234 WP_005597399.1 YgjV family protein -
  MIDG2331_RS04455 (MIDG2331_00912) folA 992021..992509 (-) 489 WP_005597400.1 type 3 dihydrofolate reductase -

Sequence

Protein


Download         Length: 400 a.a.        Molecular weight: 45618.11 Da        Isoelectric Point: 10.3239

>NTDB_id=1115300 MIDG2331_RS04430 WP_012263006.1 987012..988214(-) (pilC) [Actinobacillus pleuropneumoniae serovar 8 isolate MIDG2331]
MLKVYEFHWKAKNRFQQLQKGKALACHRTELERRLLAKGYSQIRIQRNFRFVTQASSESITQVIKQLALLLNAAVPLKQA
LNMLLENTYHIRIYMWLNDLNRLIESGYTMSSSLTKLNLYLAKSEIQLVRIGEQSGRLGEILQNIAVNRHQTEKLHKKIK
KIMFYPVVTLIISVALSLAMLLFIVPQFIELYSGREKPLPLITQMLFTLSHFLQHSLLQVTIVSLLAVGFFRFFAKKYRF
LTAIKNGMLSALPIFNKIVENVRIVFFSQNLGLMLKAHIRLETALNTFLATQSQDLRLQKEIENILAQLKQGYRFSESIN
ASVFGTQVVQMLAIGEQSGNVAQMCSYIGELYRQQLDDQIELLSQLLEPILMLIIGLIIGTVLIGLYLPIFDLGGLVGGG

Nucleotide


Download         Length: 1203 bp        

>NTDB_id=1115300 MIDG2331_RS04430 WP_012263006.1 987012..988214(-) (pilC) [Actinobacillus pleuropneumoniae serovar 8 isolate MIDG2331]
ATGTTGAAAGTGTATGAATTTCATTGGAAAGCTAAAAATCGTTTTCAACAACTTCAGAAAGGAAAAGCGTTAGCTTGTCA
TCGAACGGAATTGGAACGGCGTTTATTAGCAAAAGGCTATTCACAGATCCGTATCCAACGTAATTTTCGTTTTGTCACAC
AGGCTTCTTCAGAATCGATTACTCAAGTAATTAAACAGTTGGCGCTTTTACTGAATGCCGCAGTCCCTTTAAAACAAGCG
CTGAATATGTTGTTAGAAAATACTTATCATATTCGGATCTATATGTGGCTGAATGATTTAAACCGTTTGATAGAATCGGG
TTATACAATGTCGTCAAGTTTAACCAAATTGAATTTATATTTGGCGAAATCGGAAATACAGCTGGTACGGATTGGCGAAC
AAAGCGGGCGATTAGGCGAAATTTTGCAAAATATTGCGGTAAATCGCCATCAAACCGAAAAATTACATAAGAAAATAAAA
AAGATAATGTTTTATCCTGTCGTTACTTTGATTATTTCCGTTGCGCTTTCTCTCGCCATGCTCCTTTTTATCGTGCCGCA
ATTTATCGAGCTGTATTCCGGTCGTGAAAAGCCGCTTCCTTTAATTACACAAATGCTATTTACATTATCGCACTTCTTAC
AACATTCTTTATTACAGGTAACGATAGTCAGTTTATTAGCGGTCGGTTTTTTCCGTTTTTTTGCAAAAAAATATCGATTT
TTAACCGCTATAAAAAACGGGATGTTAAGTGCTTTGCCTATTTTTAATAAGATAGTCGAAAACGTTCGTATAGTATTTTT
TAGTCAAAATCTCGGCTTGATGCTAAAAGCTCATATTCGGCTGGAAACGGCGTTAAATACCTTTCTTGCTACACAATCGC
AAGATTTGAGGTTACAAAAAGAGATAGAAAATATTTTAGCGCAATTAAAACAAGGTTATCGCTTTTCCGAAAGTATTAAT
GCGAGTGTGTTCGGTACTCAAGTAGTACAAATGCTTGCCATTGGCGAACAGAGTGGGAATGTAGCGCAGATGTGTAGCTA
TATCGGGGAGTTATATCGACAACAATTGGATGATCAGATTGAACTGTTATCGCAGCTGCTTGAGCCGATCTTAATGCTGA
TTATCGGATTAATTATCGGTACGGTTTTGATCGGATTATATTTACCGATTTTTGATTTAGGCGGACTAGTCGGAGGCGGT
TAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(267-389)

Type II secretion system (T2SS), protein F (T2SSF)

(64-186)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B0BPG3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Glaesserella parasuis strain SC1401

50.126

99.25

0.498
  pilC Haemophilus influenzae Rd KW20

40

100

0.4
  pilC Haemophilus influenzae 86-028NP

39

100

0.39

Multiple sequence alignment