Detailed information NTG1114894

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PADK1_RS02105
• Genome accession: NZ_LN870292
• Coordinates: 443931..445079 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa DK1 substr. NH57388A
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 438931..450079

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PADK1_RS02080	-	440082..440378 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
PADK1_RS02085 (PADK1_00406)	-	440375..440968 (-)	594	WP_003084544.1	YggT family protein	-
PADK1_RS02090 (PADK1_00407)	proC	440979..441800 (-)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
PADK1_RS02095 (PADK1_00408)	-	441812..442504 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
PADK1_RS02100 (PADK1_00409)	pilT	442719..443753 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PADK1_RS02105 (PADK1_00410)	pilU	443931..445079 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
PADK1_RS02110 (PADK1_00411)	czcD	445087..445986 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PADK1_RS02115 (PADK1_00412)	-	446141..446545 (+)	405	WP_003084560.1	NINE protein	-
PADK1_RS02120 (PADK1_00413)	-	446788..448161 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
PADK1_RS02125 (PADK1_00414)	-	448158..449342 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=1114894 PADK1_RS02105 WP_003100959.1 443931..445079(+) (pilU) [Pseudomonas aeruginosa DK1 substr. NH57388A]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1114894 PADK1_RS02105 WP_003100959.1 443931..445079(+) (pilU) [Pseudomonas aeruginosa DK1 substr. NH57388A]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382