Detailed information NTG1114142

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: AT700_RS26285
• Genome accession: NZ_LN831024
• Coordinates: 5728283..5729347 (-)
• Length: 354 a.a.
• NCBI ID: WP_003110888.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain NCTC10332
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5723283..5734347

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AT700_RS26260 (ERS445055_05372)	aroK	5723818..5724336 (-)	519	WP_003095833.1	shikimate kinase AroK	-
AT700_RS26265 (ERS445055_05373)	pilQ	5724348..5726492 (-)	2145	WP_012614629.1	type 4a pilus secretin PilQ	Machinery gene
AT700_RS26270 (ERS445055_05374)	pilP	5726546..5727070 (-)	525	WP_003095836.1	type 4a pilus biogenesis lipoprotein PilP	-
AT700_RS26275 (ERS445055_05375)	pilO	5727067..5727690 (-)	624	WP_003103268.1	type 4a pilus biogenesis protein PilO	-
AT700_RS26280 (ERS445055_05376)	pilN	5727687..5728283 (-)	597	WP_003095839.1	type 4a pilus biogenesis protein PilN	-
AT700_RS26285 (ERS445055_05377)	pilM	5728283..5729347 (-)	1065	WP_003110888.1	type IV pilus assembly protein PilM	Machinery gene
AT700_RS26290 (ERS445055_05378)	-	5729532..5732003 (+)	2472	WP_003103276.1	penicillin-binding protein 1A	-
AT700_RS26295 (ERS445055_05379)	-	5732108..5733376 (-)	1269	WP_048521721.1	malic enzyme-like NAD(P)-binding protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37983.48 Da
• Isoelectric Point: 4.5405

>NTDB_id=1114142 AT700_RS26285 WP_003110888.1 5728283..5729347(-) (pilM) [Pseudomonas aeruginosa strain NCTC10332]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=1114142 AT700_RS26285 WP_003110888.1 5728283..5729347(-) (pilM) [Pseudomonas aeruginosa strain NCTC10332]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGGCTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAAAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACGCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.899	100	0.562
comM	Acinetobacter nosocomialis M2	55.899	100	0.562
comM	Acinetobacter baylyi ADP1	51.977	100	0.52
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466