Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   DR76_RS15500 Genome accession   NZ_CP009072
Coordinates   3194455..3195465 (+) Length   336 a.a.
NCBI ID   WP_000568522.1    Uniprot ID   Q8FGR3
Organism   Escherichia coli ATCC 25922     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3189455..3200465
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DR76_RS15475 (DR76_2976) nudB 3191178..3191630 (+) 453 WP_077393227.1 dihydroneopterin triphosphate diphosphatase -
  DR76_RS15480 (DR76_2977) yebC 3191659..3192399 (+) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  DR76_RS15485 (DR76_2978) ruvC 3192434..3192955 (+) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  DR76_RS15490 (DR76_2979) yebB 3192957..3193559 (-) 603 WP_001024947.1 YebB family permuted papain-like enzyme -
  DR76_RS28445 yobI 3193631..3193696 (+) 66 WP_072123646.1 stress response small protein YobI -
  DR76_RS15495 (DR76_2980) ruvA 3193835..3194446 (+) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  DR76_RS15500 (DR76_2981) ruvB 3194455..3195465 (+) 1011 WP_000568522.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  DR76_RS15505 (DR76_2982) znuB 3195612..3196397 (-) 786 WP_000571478.1 zinc ABC transporter permease subunit ZnuB -
  DR76_RS15510 (DR76_2983) znuC 3196394..3197149 (-) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  DR76_RS15515 (DR76_2984) znuA 3197228..3198160 (+) 933 WP_011076428.1 zinc ABC transporter substrate-binding protein ZnuA -
  DR76_RS15520 (DR76_2985) mepM 3198176..3199498 (+) 1323 WP_001184045.1 murein DD-endopeptidase MepM -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37171.80 Da        Isoelectric Point: 4.7818

>NTDB_id=111410 DR76_RS15500 WP_000568522.1 3194455..3195465(+) (ruvB) [Escherichia coli ATCC 25922]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TVRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=111410 DR76_RS15500 WP_000568522.1 3194455..3195465(+) (ruvB) [Escherichia coli ATCC 25922]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCGCAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTATTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAATATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCAATGCTCACTAACCTTGAACCACA
CGATGTACTGTTTATTGATGAGATCCACCGTCTTTCACCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGATTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGTGCGCGCGGTACGCCACGTATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCTGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTGGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTACTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGCGGGCGTATGGCG
ACAGTGCGGGCGTGGAACCACTTCGGCATCACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8FGR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

52.941

96.131

0.509
  ruvB Helicobacter pylori 26695

52.366

94.345

0.494