Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   VANGNB10_RS18285 Genome accession   NZ_LK021130
Coordinates   2750611..2751204 (-) Length   197 a.a.
NCBI ID   WP_013855887.1    Uniprot ID   A0A233HKC5
Organism   Vibrio anguillarum strain NB10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2745611..2756204
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VANGNB10_RS18265 (TVA0134) aroB 2746543..2747631 (-) 1089 WP_026027894.1 3-dehydroquinate synthase -
  VANGNB10_RS18270 (TVA0135) aroK 2747656..2748177 (-) 522 WP_010317691.1 shikimate kinase AroK -
  VANGNB10_RS18275 (TVA0136) pilQ 2748364..2750079 (-) 1716 WP_013855889.1 type IV pilus secretin PilQ Machinery gene
  VANGNB10_RS18280 (TVA0137) pilP 2750103..2750624 (-) 522 WP_013855888.1 pilus assembly protein PilP Machinery gene
  VANGNB10_RS18285 (TVA0138) pilO 2750611..2751204 (-) 594 WP_013855887.1 type 4a pilus biogenesis protein PilO Machinery gene
  VANGNB10_RS18290 (TVA0139) pilN 2751197..2751799 (-) 603 WP_013855886.1 PilN domain-containing protein Machinery gene
  VANGNB10_RS18295 (TVA0140) pilM 2751789..2752799 (-) 1011 WP_013855885.1 type IV pilus assembly protein PilM Machinery gene
  VANGNB10_RS18300 (TVA0141) - 2752949..2755447 (+) 2499 WP_013855884.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23039.37 Da        Isoelectric Point: 4.3879

>NTDB_id=1113449 VANGNB10_RS18285 WP_013855887.1 2750611..2751204(-) (pilO) [Vibrio anguillarum strain NB10]
MTSFQDLDMDEMAEWPLLPQLLVILVLMLIIQGVGAWFYLMPQNEQLQNLKQQEEVLKSTLRIKASKAATLPQLQAQLDE
LTERYDFLLRQLPVQKELASMLASVNELGLDNALTFTRIDWGEKQNKEFLYRLPLNIELTGNYHDIGDFSEAIAKLPRII
NFDDVDWQRVSQESSTLHFRVRAYTYQFKQEVDDEKQ

Nucleotide


Download         Length: 594 bp        

>NTDB_id=1113449 VANGNB10_RS18285 WP_013855887.1 2750611..2751204(-) (pilO) [Vibrio anguillarum strain NB10]
ATGACTAGTTTTCAAGATCTTGATATGGATGAAATGGCTGAATGGCCGTTGCTGCCTCAACTGCTGGTTATTTTGGTGTT
GATGCTGATTATTCAAGGCGTTGGCGCGTGGTTCTACCTGATGCCTCAAAATGAGCAATTGCAGAACTTAAAACAGCAAG
AAGAGGTGCTGAAATCGACCTTACGGATTAAGGCCAGCAAAGCCGCCACCTTACCCCAGTTGCAAGCTCAACTGGATGAA
TTAACTGAACGTTATGATTTTTTATTACGCCAATTACCCGTGCAAAAAGAGCTCGCAAGCATGCTCGCATCGGTCAATGA
ACTAGGGCTAGATAACGCGTTAACCTTTACACGTATTGATTGGGGAGAAAAACAGAATAAAGAGTTTCTGTATCGTTTGC
CGCTCAATATCGAATTAACTGGGAATTACCACGACATTGGTGATTTCTCAGAGGCGATCGCGAAATTGCCCCGCATTATT
AATTTCGATGATGTTGATTGGCAACGGGTTAGCCAGGAAAGCAGCACGTTACATTTTAGGGTGCGCGCTTATACCTATCA
ATTCAAGCAAGAGGTGGACGATGAAAAACAATAA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

80.612

99.492

0.802
  pilO Vibrio campbellii strain DS40M4

70.37

95.939

0.675

Multiple sequence alignment