Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   VANGNB10_RS18295 Genome accession   NZ_LK021130
Coordinates   2751789..2752799 (-) Length   336 a.a.
NCBI ID   WP_013855885.1    Uniprot ID   A0AAW4AGY3
Organism   Vibrio anguillarum strain NB10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2746789..2757799
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VANGNB10_RS18270 (TVA0135) aroK 2747656..2748177 (-) 522 WP_010317691.1 shikimate kinase AroK -
  VANGNB10_RS18275 (TVA0136) pilQ 2748364..2750079 (-) 1716 WP_013855889.1 type IV pilus secretin PilQ Machinery gene
  VANGNB10_RS18280 (TVA0137) pilP 2750103..2750624 (-) 522 WP_013855888.1 pilus assembly protein PilP Machinery gene
  VANGNB10_RS18285 (TVA0138) pilO 2750611..2751204 (-) 594 WP_013855887.1 type 4a pilus biogenesis protein PilO Machinery gene
  VANGNB10_RS18290 (TVA0139) pilN 2751197..2751799 (-) 603 WP_013855886.1 PilN domain-containing protein Machinery gene
  VANGNB10_RS18295 (TVA0140) pilM 2751789..2752799 (-) 1011 WP_013855885.1 type IV pilus assembly protein PilM Machinery gene
  VANGNB10_RS18300 (TVA0141) - 2752949..2755447 (+) 2499 WP_013855884.1 penicillin-binding protein 1A -
  VANGNB10_RS18305 (TVA0142) oxyR 2755484..2756374 (-) 891 WP_026027425.1 DNA-binding transcriptional regulator OxyR -
  VANGNB10_RS18310 (TVA0143) - 2756521..2757249 (+) 729 WP_013855882.1 redoxin family protein -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37439.00 Da        Isoelectric Point: 7.2859

>NTDB_id=1113451 VANGNB10_RS18295 WP_013855885.1 2751789..2752799(-) (pilM) [Vibrio anguillarum strain NB10]
MGKSMVTGIDIGHHSIKAVVLKLAGDSYALVGYKELFITEDIFSDNHTLDYQKIVKKLKELRKGLPLFSHKVAISVPDNA
VISKVLQIDSDLQAREKEFAIYQAFSHQSPFPIEELSLDFVKVMEKSVSRSSTVSYQVYATRKDVVESRALAAQKAGFLP
IAVDVQIHSLLNIWQLASRAQHRSDWMLMDVGHTQTALCIDFTDKAPFHKEIPLGCQWLSQDSAMSGTNAELADKNERFV
HELIERLQRQLQLFTSVNGAGSLAGIWLSGGGADTPMLAQEISRRLKLECEILDPFLLFNNKLAKRLRDKQSGQSFSTAA
GLALRGIEWLESSHVA

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=1113451 VANGNB10_RS18295 WP_013855885.1 2751789..2752799(-) (pilM) [Vibrio anguillarum strain NB10]
ATGGGTAAATCAATGGTTACGGGTATAGATATTGGCCACCATAGTATTAAAGCGGTGGTGTTAAAGCTAGCGGGTGATTC
ATATGCACTCGTCGGTTATAAAGAATTATTTATTACTGAGGACATATTCTCCGATAACCACACGCTGGATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAGAAAGGGTTTACCACTATTTAGTCACAAAGTCGCCATATCGGTACCGGACAATGCG
GTAATAAGTAAGGTATTACAGATAGATAGTGACCTGCAGGCGCGTGAAAAAGAGTTTGCTATTTATCAGGCATTTTCTCA
TCAGTCACCGTTCCCGATAGAAGAACTGAGTCTCGATTTCGTCAAAGTGATGGAAAAAAGCGTAAGTCGCAGTAGCACCG
TAAGTTATCAAGTTTACGCCACTCGTAAAGACGTCGTGGAAAGCCGTGCGTTAGCGGCTCAAAAAGCGGGCTTTTTACCG
ATCGCGGTTGATGTGCAAATACACAGTTTGTTAAACATTTGGCAGTTGGCGAGCAGGGCACAACATCGCAGCGATTGGAT
GCTCATGGATGTGGGCCATACCCAAACGGCGCTCTGTATTGATTTTACTGATAAAGCCCCTTTTCATAAAGAAATCCCTC
TCGGTTGTCAGTGGTTGTCCCAAGACAGCGCGATGTCGGGCACCAATGCCGAATTAGCCGATAAAAACGAGCGCTTTGTG
CATGAGTTGATCGAGCGACTTCAACGTCAGTTGCAGCTTTTCACCTCCGTCAATGGCGCGGGTAGTTTAGCAGGCATTTG
GCTCTCAGGTGGTGGTGCAGATACTCCGATGTTGGCGCAAGAGATTTCGCGGCGTCTCAAGCTTGAGTGCGAAATCCTCG
ATCCCTTCCTTCTGTTTAACAATAAGTTAGCGAAACGCCTGCGAGATAAGCAGTCTGGGCAGAGTTTTAGCACCGCGGCT
GGCCTTGCACTGCGTGGTATCGAGTGGTTGGAGTCTTCTCATGTTGCATAG

Domains

Predicted by InterProScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

67.456

100

0.679
  pilM Vibrio campbellii strain DS40M4

52.381

100

0.524

Multiple sequence alignment