Detailed information NTG1111926

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PKB_RS14085
• Genome accession: NZ_HG322950
• Coordinates: 2984756..2985967 (-)
• Length: 403 a.a.
• NCBI ID: WP_197539260.1
• Uniprot ID: A0A024HH63
• Organism: Pseudomonas knackmussii B13
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2979756..2990967

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PKB_RS14065 (PKB_2860)	-	2979948..2980763 (-)	816	WP_043252655.1	sugar phosphate isomerase/epimerase	-
PKB_RS14070 (PKB_2861)	-	2980810..2981862 (-)	1053	WP_043252658.1	Gfo/Idh/MocA family oxidoreductase	-
PKB_RS14075 (PKB_2862)	-	2982070..2982846 (-)	777	WP_043252660.1	IclR family transcriptional regulator	-
PKB_RS14080 (PKB_2863)	-	2983232..2984494 (+)	1263	WP_052355284.1	OprD family porin	-
PKB_RS14085 (PKB_2864)	pilU	2984756..2985967 (-)	1212	WP_197539260.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PKB_RS14090 (PKB_2865)	-	2986160..2986438 (+)	279	WP_043252662.1	peptidylprolyl isomerase	-
PKB_RS14095 (PKB_2866)	-	2986457..2986819 (+)	363	WP_043252664.1	MmcQ/YjbR family DNA-binding protein	-
PKB_RS14100 (PKB_2867)	-	2986816..2987373 (+)	558	WP_043252667.1	putative glycolipid-binding domain-containing protein	-
PKB_RS14105 (PKB_2868)	-	2987662..2988843 (+)	1182	WP_043252670.1	MFS transporter	-
PKB_RS14110 (PKB_2869)	-	2989044..2990324 (+)	1281	WP_043252672.1	OprD family porin	-

Sequence

Protein

• Length: 403 a.a.
• Molecular weight: 44280.68 Da
• Isoelectric Point: 6.1322

>NTDB_id=1111926 PKB_RS14085 WP_197539260.1 2984756..2985967(-) (pilU) [Pseudomonas knackmussii B13]
MEIQAMLRILSSQDGSDLYLSTGAPPCAKFNGVLKPLSNEPLKAGEVAAIANSIMDEEQRAEFERELEMNLAISVTGVGR
FRINIFKQRNEVSIVARNIKLDIPLFEDLKLPEVLLKVVMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIVTIED
PIEYVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTQDGKRRAAVEVLLGTATISDIIKRGEFSMIKEIMDKSRALGMQTFDQAL
FDLAVEGAISEEEAVKNADSANNLRLKLKLYKDNPATAMAQAAKAQPATPTPAPAAPVEASSWGLELALEEIKAEDEEGK
PGI

Nucleotide

• Length: 1212 bp

>NTDB_id=1111926 PKB_RS14085 WP_197539260.1 2984756..2985967(-) (pilU) [Pseudomonas knackmussii B13]
ATGGAAATCCAAGCGATGCTGCGGATCCTGTCCAGTCAGGACGGTTCCGACCTCTACCTGTCCACCGGCGCGCCGCCCTG
CGCCAAGTTCAACGGTGTGCTCAAGCCCCTGTCCAACGAACCGCTGAAAGCCGGCGAAGTCGCGGCCATCGCCAACTCGA
TCATGGACGAGGAGCAGCGCGCCGAATTCGAACGCGAGCTGGAGATGAACCTGGCGATCTCGGTCACCGGCGTCGGGCGC
TTCCGCATCAACATCTTCAAGCAGCGCAACGAGGTTTCCATCGTTGCGCGGAACATCAAGCTGGACATCCCGCTGTTCGA
GGACCTGAAGCTGCCGGAGGTGCTGCTCAAGGTGGTGATGGAGAAGCGTGGCCTGGTGCTCTTCGTCGGCGGCACCGGCT
CGGGCAAGTCGACCTCCCTGGCGGCGCTGATCGACCACCGCAACCGCAACAGCGGCGGCCACATCGTCACCATCGAAGAC
CCCATCGAGTACGTGCACCGGCACCGCAAGTCGATCATCAACCAGCGCGAAGTGGGCGTGGACACCCGCAGCTTCCATGC
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGCGACCGCGAGACCATGGAGCACG
CCCTGGCCTTCGCCGACACCGGCCACCTGGCGATCTCGACGCTCCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAGGAGCGTCGTCCGCAACTGCTCAACGACCTGGGCAACAACCTCAAGGCGTTCGTCTCGCAGCG
CCTGGTGCGCACCCAGGACGGCAAGCGCCGCGCCGCGGTGGAGGTGCTACTGGGCACCGCGACCATCAGCGACATCATCA
AGCGCGGCGAATTCAGCATGATCAAGGAGATCATGGACAAGTCCCGCGCCCTCGGCATGCAGACCTTCGACCAGGCGCTG
TTCGACCTGGCCGTGGAAGGCGCGATCAGCGAAGAGGAAGCGGTGAAGAACGCCGACTCGGCGAACAACCTGCGCCTGAA
GCTGAAGCTCTACAAGGACAACCCGGCCACCGCCATGGCCCAGGCGGCCAAGGCCCAGCCCGCCACTCCGACGCCCGCGC
CAGCGGCCCCGGTGGAGGCTTCCAGCTGGGGCCTGGAACTGGCCCTGGAAGAGATCAAGGCCGAGGACGAGGAAGGCAAG
CCGGGCATTTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (110-271)

3D structure

• AlphaFold DB: A0A024HH63

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.98	87.097	0.496
pilU	Acinetobacter baylyi ADP1	53.693	87.345	0.469
pilU	Vibrio cholerae strain A1552	52.286	86.849	0.454
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.104	95.285	0.382