Detailed information NTG1111128

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACODUA_RS02255
• Genome accession: NZ_CP184748
• Coordinates: 497288..498463 (-)
• Length: 391 a.a.
• NCBI ID: WP_014146965.1
• Uniprot ID: -
• Organism: Methylotuvimicrobium alcaliphilum 20Z
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 492288..503463

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACODUA_RS02245 (ACODUA_02245)	-	493099..495438 (-)	2340	WP_014146963.1	alpha/beta fold hydrolase	-
ACODUA_RS02250 (ACODUA_02250)	-	495947..496438 (+)	492	WP_084685258.1	hypothetical protein	-
ACODUA_RS02255 (ACODUA_02255)	pilU	497288..498463 (-)	1176	WP_014146965.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACODUA_RS02260 (ACODUA_02260)	-	498977..500020 (+)	1044	WP_014146966.1	tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase	-
ACODUA_RS02265 (ACODUA_02265)	-	500017..500244 (+)	228	WP_014146967.1	sulfurtransferase TusA family protein	-
ACODUA_RS02270 (ACODUA_02270)	-	500244..501386 (+)	1143	WP_014146968.1	cation diffusion facilitator family transporter	-
ACODUA_RS02275 (ACODUA_02275)	argJ	501850..503064 (+)	1215	WP_014146969.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-

Sequence

Protein

• Length: 391 a.a.
• Molecular weight: 43452.60 Da
• Isoelectric Point: 5.4953

>NTDB_id=1111128 ACODUA_RS02255 WP_014146965.1 497288..498463(-) (pilU) [Methylotuvimicrobium alcaliphilum 20Z]
MDFKDYLKILVNKDGSDLYLTVGAPAAAKFQGALKPLESEKLTGERIKAIAYSLMDADQQKDFEHVPEMNLAITEQGIGR
FRVNIFKQRNNLALVIRNIKVDIPNADQLGLPEVLKKVIMEKRGLILFVGGTGSGKSTSLAALIDHRNSNSSGHIITIED
PIEYLHPHKRCLVNQREVGVDTLSYEDALKNTLRQAPDVILIGEIRSQETMEHALAFAETGHLCLSTLHANNANQALDRI
INFFPEERRNQLLLDLSLNLKAFVSQRLVPTVDGKRVAAIEILLGTQLVKDLIHKGEIHTIKEAMEKSENIGMQTFDSHL
LKLFKEGKISLEEALQNSDSPNNLKLKINLSEGKGSATEAVAKSTGGLTSRFSLEAIEKDQEEEEEEEEQS

Nucleotide

• Length: 1176 bp

>NTDB_id=1111128 ACODUA_RS02255 WP_014146965.1 497288..498463(-) (pilU) [Methylotuvimicrobium alcaliphilum 20Z]
ATGGATTTTAAAGATTATCTAAAAATTTTGGTCAACAAAGACGGTTCCGATCTTTACTTAACGGTCGGCGCGCCGGCCGC
GGCAAAATTTCAAGGCGCATTGAAACCGCTCGAAAGCGAAAAACTGACCGGCGAACGCATCAAGGCTATCGCTTACAGTT
TAATGGATGCCGATCAGCAAAAAGACTTCGAACACGTCCCGGAAATGAATCTGGCGATCACCGAACAGGGCATCGGCCGA
TTCCGGGTCAATATTTTCAAACAGCGCAACAACCTGGCCTTGGTCATTCGAAACATCAAGGTCGATATTCCGAATGCCGA
TCAATTAGGCCTGCCCGAAGTGCTGAAAAAAGTCATCATGGAAAAGCGCGGTTTGATTCTATTCGTCGGCGGCACCGGCT
CCGGCAAGTCGACCTCCCTGGCCGCACTGATCGATCACCGCAACAGCAATTCGTCGGGGCACATCATCACGATCGAAGAC
CCGATCGAATATCTTCATCCGCACAAACGCTGCCTGGTCAATCAACGCGAAGTCGGCGTCGATACGCTCAGTTACGAGGA
CGCGTTGAAAAATACGCTACGCCAGGCACCGGACGTGATTCTGATCGGCGAAATCCGCTCTCAAGAAACGATGGAACATG
CACTCGCCTTCGCCGAAACCGGGCATTTATGCTTGTCGACGCTGCACGCCAACAACGCCAATCAGGCCCTGGACCGTATC
ATCAATTTCTTCCCGGAAGAGCGGCGTAACCAATTATTGCTGGATCTATCGCTGAATTTGAAGGCCTTTGTTTCGCAAAG
ACTTGTTCCTACCGTGGACGGAAAACGGGTCGCGGCGATCGAAATTTTGCTGGGGACGCAATTGGTCAAAGACTTGATTC
ATAAAGGCGAAATTCATACGATCAAGGAAGCGATGGAAAAATCCGAAAACATCGGCATGCAAACCTTCGACAGCCATTTG
CTCAAACTTTTCAAGGAAGGCAAAATTTCACTCGAAGAAGCCTTGCAAAACTCCGACTCGCCGAATAACTTGAAATTGAA
AATCAACCTCAGCGAAGGCAAGGGATCGGCGACCGAAGCGGTCGCTAAATCGACTGGCGGCTTGACTTCAAGATTCTCGC
TCGAGGCGATTGAAAAGGATCAGGAGGAAGAAGAAGAAGAAGAAGAGCAAAGCTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (69-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56	95.908	0.537
pilU	Acinetobacter baylyi ADP1	53.955	90.537	0.488
pilU	Vibrio cholerae strain A1552	50.847	90.537	0.46
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.667	92.072	0.384