Detailed information NTG1111106

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACODT9_RS02255
• Genome accession: NZ_CP184747
• Coordinates: 497342..498517 (-)
• Length: 391 a.a.
• NCBI ID: WP_014146965.1
• Uniprot ID: -
• Organism: Methylotuvimicrobium alcaliphilum strain 20ZK
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 492342..503517

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACODT9_RS02245 (ACODT9_02245)	-	493153..495492 (-)	2340	WP_014146963.1	alpha/beta fold hydrolase	-
ACODT9_RS02250 (ACODT9_02250)	-	496001..496492 (+)	492	WP_084685258.1	hypothetical protein	-
ACODT9_RS02255 (ACODT9_02255)	pilU	497342..498517 (-)	1176	WP_014146965.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACODT9_RS02260 (ACODT9_02260)	-	499031..500074 (+)	1044	WP_014146966.1	tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase	-
ACODT9_RS02265 (ACODT9_02265)	-	500071..500298 (+)	228	WP_014146967.1	sulfurtransferase TusA family protein	-
ACODT9_RS02270 (ACODT9_02270)	-	500298..501440 (+)	1143	WP_014146968.1	cation diffusion facilitator family transporter	-
ACODT9_RS02275 (ACODT9_02275)	argJ	501904..503118 (+)	1215	WP_014146969.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-

Sequence

Protein

• Length: 391 a.a.
• Molecular weight: 43452.60 Da
• Isoelectric Point: 5.4953

>NTDB_id=1111106 ACODT9_RS02255 WP_014146965.1 497342..498517(-) (pilU) [Methylotuvimicrobium alcaliphilum strain 20ZK]
MDFKDYLKILVNKDGSDLYLTVGAPAAAKFQGALKPLESEKLTGERIKAIAYSLMDADQQKDFEHVPEMNLAITEQGIGR
FRVNIFKQRNNLALVIRNIKVDIPNADQLGLPEVLKKVIMEKRGLILFVGGTGSGKSTSLAALIDHRNSNSSGHIITIED
PIEYLHPHKRCLVNQREVGVDTLSYEDALKNTLRQAPDVILIGEIRSQETMEHALAFAETGHLCLSTLHANNANQALDRI
INFFPEERRNQLLLDLSLNLKAFVSQRLVPTVDGKRVAAIEILLGTQLVKDLIHKGEIHTIKEAMEKSENIGMQTFDSHL
LKLFKEGKISLEEALQNSDSPNNLKLKINLSEGKGSATEAVAKSTGGLTSRFSLEAIEKDQEEEEEEEEQS

Nucleotide

• Length: 1176 bp

>NTDB_id=1111106 ACODT9_RS02255 WP_014146965.1 497342..498517(-) (pilU) [Methylotuvimicrobium alcaliphilum strain 20ZK]
ATGGATTTTAAAGATTATCTAAAAATTTTGGTCAACAAAGACGGTTCCGATCTTTACTTAACGGTCGGCGCGCCGGCCGC
GGCAAAATTTCAAGGCGCATTGAAACCGCTCGAAAGCGAAAAACTGACCGGCGAACGCATCAAGGCTATCGCTTACAGTT
TAATGGATGCCGATCAGCAAAAAGACTTCGAACACGTCCCGGAAATGAATCTGGCGATCACCGAACAGGGCATCGGCCGA
TTCCGGGTCAATATTTTCAAACAGCGCAACAACCTGGCCTTGGTCATTCGAAACATCAAGGTCGATATTCCGAATGCCGA
TCAATTAGGCCTGCCCGAAGTGCTGAAAAAAGTCATCATGGAAAAGCGCGGTTTGATTCTATTCGTCGGCGGCACCGGCT
CCGGCAAGTCGACCTCCCTGGCCGCACTGATCGATCACCGCAACAGCAATTCGTCGGGGCACATCATCACGATCGAAGAC
CCGATCGAATATCTTCATCCGCACAAACGCTGCCTGGTCAATCAACGCGAAGTCGGCGTCGATACGCTCAGTTACGAGGA
CGCGTTGAAAAATACGCTACGCCAGGCACCGGACGTGATTCTGATCGGCGAAATCCGCTCTCAAGAAACGATGGAACATG
CACTCGCCTTCGCCGAAACCGGGCATTTATGCTTGTCGACGCTGCACGCCAACAACGCCAATCAGGCCCTGGACCGTATC
ATCAATTTCTTCCCGGAAGAGCGGCGTAACCAATTATTGCTGGATCTATCGCTGAATTTGAAGGCCTTTGTTTCGCAAAG
ACTTGTTCCTACCGTGGACGGAAAACGGGTCGCGGCGATCGAAATTTTGCTGGGGACGCAATTGGTCAAAGACTTGATTC
ATAAAGGCGAAATTCATACGATCAAGGAAGCGATGGAAAAATCCGAAAACATCGGCATGCAAACCTTCGACAGCCATTTG
CTCAAACTTTTCAAGGAAGGCAAAATTTCACTCGAAGAAGCCTTGCAAAACTCCGACTCGCCGAATAACTTGAAATTGAA
AATCAACCTCAGCGAAGGCAAGGGATCGGCGACCGAAGCGGTCGCTAAATCGACTGGCGGCTTGACTTCAAGATTCTCGC
TCGAGGCGATTGAAAAGGATCAGGAGGAAGAAGAAGAAGAAGAAGAGCAAAGCTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (69-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56	95.908	0.537
pilU	Acinetobacter baylyi ADP1	53.955	90.537	0.488
pilU	Vibrio cholerae strain A1552	50.847	90.537	0.46
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.667	92.072	0.384