Detailed information NTG1110408

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACODFA_RS04965
• Genome accession: NZ_CP184642
• Coordinates: 1022875..1024023 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain 0211
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1017875..1029023

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACODFA_RS04940 (ACODFA_04940)	-	1019026..1019322 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
ACODFA_RS04945 (ACODFA_04945)	-	1019319..1019912 (-)	594	WP_003084544.1	YggT family protein	-
ACODFA_RS04950 (ACODFA_04950)	proC	1019923..1020744 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
ACODFA_RS04955 (ACODFA_04955)	-	1020756..1021448 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACODFA_RS04960 (ACODFA_04960)	pilT	1021663..1022697 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
ACODFA_RS04965 (ACODFA_04965)	pilU	1022875..1024023 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
ACODFA_RS04970 (ACODFA_04970)	czcD	1024031..1024930 (-)	900	WP_003084557.1	CDF family zinc transporter CzcD	-
ACODFA_RS04975 (ACODFA_04975)	-	1025085..1025489 (+)	405	WP_003084560.1	NINE protein	-
ACODFA_RS04980 (ACODFA_04980)	-	1025732..1027105 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
ACODFA_RS04985 (ACODFA_04985)	-	1027102..1028286 (+)	1185	WP_003084564.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=1110408 ACODFA_RS04965 WP_003100959.1 1022875..1024023(+) (pilU) [Pseudomonas aeruginosa strain 0211]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1110408 ACODFA_RS04965 WP_003100959.1 1022875..1024023(+) (pilU) [Pseudomonas aeruginosa strain 0211]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAATTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACTCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382