Detailed information NTG1110370

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACOCHX_RS14385
• Genome accession: NZ_CP184613
• Coordinates: 2983422..2984540 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain CQPMC-AB10
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2978422..2989540

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACOCHX_RS14355 (ACOCHX_14355)	-	2979251..2980099 (+)	849	WP_000336548.1	class II glutamine amidotransferase	-
ACOCHX_RS14360 (ACOCHX_14360)	-	2980352..2980807 (+)	456	WP_000782976.1	bacteriohemerythrin	-
ACOCHX_RS14365 (ACOCHX_14365)	-	2980960..2982027 (+)	1068	WP_002019572.1	hypothetical protein	-
ACOCHX_RS14370 (ACOCHX_14370)	-	2982027..2982350 (+)	324	WP_000442587.1	RnfH family protein	-
ACOCHX_RS14375 (ACOCHX_14375)	-	2982405..2982803 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
ACOCHX_RS14380 (ACOCHX_14380)	fur	2982915..2983352 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
ACOCHX_RS14385 (ACOCHX_14385)	pilU	2983422..2984540 (-)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACOCHX_RS14390 (ACOCHX_14390)	pilT	2984568..2985605 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
ACOCHX_RS14395 (ACOCHX_14395)	-	2985732..2986424 (+)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=1110370 ACOCHX_RS14385 WP_000347039.1 2983422..2984540(-) (pilU) [Acinetobacter baumannii strain CQPMC-AB10]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=1110370 ACOCHX_RS14385 WP_000347039.1 2983422..2984540(-) (pilU) [Acinetobacter baumannii strain CQPMC-AB10]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395