Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACOCIB_RS14340 Genome accession   NZ_CP184593
Coordinates   2987234..2988352 (+) Length   372 a.a.
NCBI ID   WP_000347039.1    Uniprot ID   A0A9P2XLL8
Organism   Acinetobacter baumannii strain CQPMC-AB22     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2982234..2993352
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOCIB_RS14330 (ACOCIB_14330) - 2985350..2986042 (-) 693 WP_001108512.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACOCIB_RS14335 (ACOCIB_14335) pilT 2986169..2987206 (+) 1038 WP_000355489.1 type IV pilus twitching motility protein PilT Machinery gene
  ACOCIB_RS14340 (ACOCIB_14340) pilU 2987234..2988352 (+) 1119 WP_000347039.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACOCIB_RS14345 (ACOCIB_14345) fur 2988422..2988859 (-) 438 WP_001122847.1 ferric iron uptake transcriptional regulator -
  ACOCIB_RS14350 (ACOCIB_14350) - 2988971..2989369 (+) 399 WP_001170994.1 outer membrane protein assembly factor BamE -
  ACOCIB_RS14355 (ACOCIB_14355) - 2989424..2989747 (-) 324 WP_000442587.1 RnfH family protein -
  ACOCIB_RS14360 (ACOCIB_14360) - 2989747..2990814 (-) 1068 WP_002019572.1 hypothetical protein -
  ACOCIB_RS14365 (ACOCIB_14365) - 2990967..2991422 (-) 456 WP_000782976.1 bacteriohemerythrin -
  ACOCIB_RS14370 (ACOCIB_14370) - 2991804..2992091 (-) 288 WP_001218560.1 PA4642 family protein -
  ACOCIB_RS14375 (ACOCIB_14375) minE 2992175..2992447 (-) 273 WP_000896934.1 cell division topological specificity factor MinE -
  ACOCIB_RS14380 (ACOCIB_14380) minD 2992450..2993262 (-) 813 WP_001074362.1 septum site-determining protein MinD -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41933.13 Da        Isoelectric Point: 6.6944

>NTDB_id=1109946 ACOCIB_RS14340 WP_000347039.1 2987234..2988352(+) (pilU) [Acinetobacter baumannii strain CQPMC-AB22]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=1109946 ACOCIB_RS14340 WP_000347039.1 2987234..2988352(+) (pilU) [Acinetobacter baumannii strain CQPMC-AB22]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(31-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.926

100

0.772
  pilU Pseudomonas stutzeri DSM 10701

69.565

98.925

0.688
  pilU Vibrio cholerae strain A1552

53.243

99.462

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.486

93.011

0.395

Multiple sequence alignment