Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACN19N_RS15650 Genome accession   NZ_CP184160
Coordinates   3252279..3253460 (+) Length   393 a.a.
NCBI ID   WP_269229228.1    Uniprot ID   A0A9E9NNI4
Organism   Acinetobacter sp. LF10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3247279..3258460
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN19N_RS15620 (ACN19N_15620) - 3247542..3247856 (-) 315 WP_269238787.1 hypothetical protein -
  ACN19N_RS15625 (ACN19N_15625) - 3247870..3248343 (-) 474 WP_269238788.1 hypothetical protein -
  ACN19N_RS15630 (ACN19N_15630) guaD 3248531..3249847 (+) 1317 WP_293737884.1 guanine deaminase -
  ACN19N_RS15635 (ACN19N_15635) hpt 3250072..3250599 (+) 528 WP_018679859.1 hypoxanthine phosphoribosyltransferase -
  ACN19N_RS15640 (ACN19N_15640) - 3250832..3251083 (+) 252 WP_004660536.1 GlsB/YeaQ/YmgE family stress response membrane protein -
  ACN19N_RS15645 (ACN19N_15645) - 3251142..3251840 (-) 699 WP_269229227.1 DsbC family protein -
  ACN19N_RS15650 (ACN19N_15650) pilU 3252279..3253460 (+) 1182 WP_269229228.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACN19N_RS15655 (ACN19N_15655) - 3253536..3253871 (-) 336 WP_004660542.1 hypothetical protein -
  ACN19N_RS15660 (ACN19N_15660) mnmE 3254226..3255581 (-) 1356 WP_269229231.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE -
  ACN19N_RS15665 (ACN19N_15665) yidC 3255696..3257456 (-) 1761 WP_269229232.1 membrane protein insertase YidC -
  ACN19N_RS15670 (ACN19N_15670) yidD 3257459..3257782 (-) 324 WP_269229233.1 membrane protein insertion efficiency factor YidD -
  ACN19N_RS15675 (ACN19N_15675) rnpA 3257792..3258187 (-) 396 WP_420001729.1 ribonuclease P protein component -
  ACN19N_RS15680 (ACN19N_15680) rpmH 3258215..3258349 (-) 135 WP_000831329.1 50S ribosomal protein L34 -

Sequence

Protein


Download         Length: 393 a.a.        Molecular weight: 44609.15 Da        Isoelectric Point: 6.3751

>NTDB_id=1108037 ACN19N_RS15650 WP_269229228.1 3252279..3253460(+) (pilU) [Acinetobacter sp. LF10]
MYNSELLEEAKTFMFHMLTKVVEYGGSDLFITADFPPSIKLQGLMRPLGQQILTAEKTKLFAYSLMNEKQRQEFENEWEC
NFAINIPDVSRFRVNVFKQQLQIGMVIRTITSEIPTFQKLKLPESLKNVMMEKRGLVLVVGSTGSGKSTSLAAMIDYRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHTWHHALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQALDRIINFFPDERRNQLLMDLSSNMKAIISQRLVRTQDGQGRRAAVEILLNTPLIAENILKGQFHNLKEIMTKS
RELGMQTFDQALFDLYNEGAISYDEALRNADSLNELRLQIKLKSTRQEGVPASAATSLNMLKEDSEEQEMQGV

Nucleotide


Download         Length: 1182 bp        

>NTDB_id=1108037 ACN19N_RS15650 WP_269229228.1 3252279..3253460(+) (pilU) [Acinetobacter sp. LF10]
ATGTATAATTCGGAACTATTAGAAGAAGCCAAAACGTTTATGTTTCATATGCTGACCAAAGTGGTCGAATATGGCGGCTC
AGATTTATTTATTACAGCAGACTTTCCACCGAGTATAAAATTACAAGGTTTGATGCGTCCGTTGGGACAGCAAATCTTAA
CAGCAGAAAAAACCAAGCTATTTGCATATAGCCTAATGAATGAAAAACAACGCCAAGAGTTTGAAAACGAATGGGAATGT
AATTTCGCAATCAACATTCCTGATGTATCTCGTTTTCGTGTTAATGTATTTAAGCAACAACTACAAATCGGCATGGTCAT
CCGAACTATTACCTCAGAAATACCCACTTTCCAAAAGCTCAAACTTCCAGAATCTTTGAAAAATGTCATGATGGAAAAAC
GTGGCTTAGTATTAGTCGTAGGAAGTACGGGGTCAGGTAAATCAACCTCATTAGCGGCAATGATTGATTATCGTAATGAA
AATTCGGCGGGGCATATTATTACGGTTGAAGACCCCGTGGAATATGTACACAAGCATAAGAAATCAATGATTACCCACCG
AGAAGTAGGTGTAGATTGCCATACTTGGCACCATGCCTTAAAAAACACTTTGCGTCAGGCACCTGATGTAATTCTGATCG
GTGAAATCCGTGATACTGAAACCATGGAACATGCGATTGCTTTCGCTGAAACAGGGCATCTGTGTTTAGGGACGCTCCAC
GCAAACAATGCCAACCAAGCCTTAGATCGAATTATCAACTTCTTTCCAGATGAACGTCGCAATCAATTACTAATGGATCT
TTCATCCAATATGAAAGCCATTATTTCTCAACGCTTGGTCCGTACTCAAGATGGTCAAGGTCGTCGTGCAGCCGTAGAGA
TTCTACTGAATACACCATTAATTGCAGAAAATATTTTAAAAGGTCAATTTCATAATCTTAAAGAAATTATGACTAAATCT
CGTGAATTAGGTATGCAAACTTTTGATCAGGCCTTGTTTGATTTATATAACGAAGGTGCAATTAGTTATGATGAAGCGTT
ACGTAATGCTGACTCATTGAATGAGTTACGCTTACAAATTAAGCTGAAAAGTACTCGTCAAGAAGGTGTTCCAGCCAGTG
CAGCAACTTCTTTAAATATGCTGAAAGAAGACTCTGAAGAGCAAGAAATGCAAGGCGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.345

88.55

0.534
  pilU Acinetobacter baylyi ADP1

55.908

88.295

0.494
  pilU Vibrio cholerae strain A1552

52.601

88.041

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.515

84.987

0.361

Multiple sequence alignment