Detailed information NTG1106220

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: ACM62G_RS09745
• Genome accession: NZ_CP183808
• Coordinates: 2109330..2110394 (-)
• Length: 354 a.a.
• NCBI ID: WP_054059111.1
• Uniprot ID: A0A0N0E5G0
• Organism: Pseudomonas sp. COR18
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2104330..2115394

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACM62G_RS09720 (ACM62G_09720)	aroK	2105011..2105529 (-)	519	WP_026145113.1	shikimate kinase AroK	-
ACM62G_RS09725 (ACM62G_09725)	pilQ	2105534..2107608 (-)	2075	Protein_1915	type IV pilus secretin PilQ	-
ACM62G_RS09730 (ACM62G_09730)	pilP	2107620..2108147 (-)	528	WP_054059114.1	type 4a pilus biogenesis lipoprotein PilP	-
ACM62G_RS09735 (ACM62G_09735)	pilO	2108144..2108767 (-)	624	WP_054059113.1	type 4a pilus biogenesis protein PilO	-
ACM62G_RS09740 (ACM62G_09740)	-	2108764..2109330 (-)	567	WP_419737076.1	PilN domain-containing protein	-
ACM62G_RS09745 (ACM62G_09745)	pilM	2109330..2110394 (-)	1065	WP_054059111.1	pilus assembly protein PilM	Machinery gene
ACM62G_RS09750 (ACM62G_09750)	-	2110601..2113033 (+)	2433	WP_419737506.1	penicillin-binding protein 1A	-
ACM62G_RS09755 (ACM62G_09755)	-	2113237..2114505 (-)	1269	WP_186687223.1	malic enzyme-like NAD(P)-binding protein	-
ACM62G_RS09760 (ACM62G_09760)	rpmE	2114848..2115066 (-)	219	WP_054059108.1	50S ribosomal protein L31	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38114.49 Da
• Isoelectric Point: 4.5877

>NTDB_id=1106220 ACM62G_RS09745 WP_054059111.1 2109330..2110394(-) (pilM) [Pseudomonas sp. COR18]
MLGLFGKKAHSLLGIDISSTSVKLLELSRSGNRYRVESYAVEPLPPNAVVEKNIAELEGVGQALTRVLLKARTSAREAAV
AVAGSAVITKTIEMDAGLSDDDMESQLKIEADQYIPYPLEEVAIDFEVQGYSARNPERVEVLLAACRKENVEVREAALGL
AGLDARVVDVEAYALERSFGLLASQLAIDHDKLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLSN
EEAGLAKKQGGLPDDYVVEVLQPFKDALVQQVSRSLQFFFASGQYNDVDYILLAGGTASIAGLDHLIQQRLGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=1106220 ACM62G_RS09745 WP_054059111.1 2109330..2110394(-) (pilM) [Pseudomonas sp. COR18]
GTGCTTGGACTCTTCGGCAAAAAAGCGCATTCGCTCCTGGGCATAGACATCAGTTCCACCTCGGTCAAGCTCCTTGAATT
GAGCCGCTCGGGCAATCGATATCGGGTCGAGTCCTATGCCGTGGAACCGCTGCCGCCCAATGCCGTGGTCGAGAAGAACA
TTGCCGAACTCGAAGGCGTCGGCCAGGCCCTGACGCGAGTGCTGCTCAAGGCCCGGACCAGTGCACGCGAAGCCGCGGTG
GCGGTGGCCGGTTCCGCGGTCATCACCAAGACCATCGAAATGGATGCCGGGCTCTCCGATGACGACATGGAGAGCCAGCT
CAAGATCGAAGCCGACCAGTACATTCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGGTATTCGGCGC
GCAACCCCGAGCGGGTCGAGGTGCTGTTGGCCGCCTGTCGGAAAGAAAATGTCGAGGTGCGCGAGGCGGCCCTCGGCCTG
GCCGGGCTCGATGCCCGGGTGGTCGATGTCGAGGCTTATGCGCTGGAGCGCTCCTTTGGCCTGCTGGCGTCCCAGTTGGC
CATCGACCACGACAAGCTGACGGTCGCGGTGGTCGATATCGGTGCGACCATGACCACCCTCAGCGTCCTGCATAACGGGC
GGATCATCTATACCCGCGAGCAATTGTTCGGCGGACGCCAGCTTACCGAGGAAATCCAGCGGCGCTATGGCCTTTCCAAC
GAGGAGGCGGGGCTGGCGAAGAAGCAGGGCGGCCTGCCCGACGATTATGTCGTCGAGGTCCTGCAGCCGTTCAAGGATGC
TCTGGTCCAGCAGGTGTCGCGCTCCCTGCAGTTCTTCTTCGCCTCGGGCCAGTACAACGATGTCGACTACATCCTGCTGG
CCGGCGGCACCGCCTCCATTGCCGGGCTCGATCACCTGATCCAGCAGCGGCTGGGCACGCCGACCATGGTCGCCAACCCT
TTCGCCGACATGGCGTTGAGCAGCAAGGTCAATGCCGGGGCACTGGCCAGCGATGCGCCAGCCCTGATGATCGCCTGTGG
CCTGGCGTTGAGGAGTTTTGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A0N0E5G0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	57.345	100	0.573
comM	Acinetobacter nosocomialis M2	57.345	100	0.573
comM	Acinetobacter baylyi ADP1	55.65	100	0.557
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466