Detailed information NTG0110565

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACAM36_RS02140
• Genome accession: NZ_AP031604
• Coordinates: 471860..473008 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa isolate PaSz
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 466860..478008

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACAM36_RS02115	-	468011..468307 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
ACAM36_RS02120 (RVB5_04240)	-	468304..468897 (-)	594	WP_003084544.1	YggT family protein	-
ACAM36_RS02125 (RVB5_04250)	proC	468908..469729 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
ACAM36_RS02130 (RVB5_04260)	-	469741..470433 (-)	693	WP_003118803.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACAM36_RS02135 (RVB5_04270)	pilT	470648..471682 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
ACAM36_RS02140 (RVB5_04280)	pilU	471860..473008 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
ACAM36_RS02145 (RVB5_04290)	czcD	473016..473915 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
ACAM36_RS02150 (RVB5_04300)	-	474069..474473 (+)	405	WP_003084560.1	NINE protein	-
ACAM36_RS02155 (RVB5_04310)	-	474716..476089 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
ACAM36_RS02160 (RVB5_04320)	-	476086..477270 (+)	1185	WP_003117897.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=110565 ACAM36_RS02140 WP_003100959.1 471860..473008(+) (pilU) [Pseudomonas aeruginosa isolate PaSz]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=110565 ACAM36_RS02140 WP_003100959.1 471860..473008(+) (pilU) [Pseudomonas aeruginosa isolate PaSz]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACTAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382