Detailed information NTG0110418

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACAK38_RS15000
• Genome accession: NZ_AP031583
• Coordinates: 3108192..3109310 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain JUNP514
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3103192..3114310

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACAK38_RS14990 (JUNP514_2994)	-	3106308..3107000 (-)	693	WP_001108516.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACAK38_RS14995 (JUNP514_2995)	pilT	3107127..3108164 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
ACAK38_RS15000 (JUNP514_2996)	pilU	3108192..3109310 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACAK38_RS15005 (JUNP514_2997)	fur	3109380..3109817 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
ACAK38_RS15010 (JUNP514_2998)	-	3109929..3110327 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
ACAK38_RS15015 (JUNP514_2999)	-	3110382..3110705 (-)	324	WP_000442590.1	RnfH family protein	-
ACAK38_RS15020 (JUNP514_3000)	-	3110705..3111772 (-)	1068	WP_004748097.1	hypothetical protein	-
ACAK38_RS15025 (JUNP514_3001)	-	3111925..3112380 (-)	456	WP_000782976.1	bacteriohemerythrin	-
ACAK38_RS15030 (JUNP514_3002)	-	3112631..3113479 (-)	849	WP_025469635.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=110418 ACAK38_RS15000 WP_000347036.1 3108192..3109310(+) (pilU) [Acinetobacter baumannii strain JUNP514]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=110418 ACAK38_RS15000 WP_000347036.1 3108192..3109310(+) (pilU) [Acinetobacter baumannii strain JUNP514]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCTTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTAAGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAATAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392