Detailed information NTG1103891

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACNOHD_RS01515
• Genome accession: NZ_CP183060
• Coordinates: 322840..323985 (+)
• Length: 381 a.a.
• NCBI ID: WP_014818747.1
• Uniprot ID: I4CN99
• Organism: Stutzerimonas kunmingensis strain L4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 317840..328985

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACNOHD_RS01495 (ACNOHD_01495)	-	319515..320105 (-)	591	WP_014818744.1	YggT family protein	-
ACNOHD_RS01500 (ACNOHD_01500)	proC	320156..320980 (-)	825	WP_014818745.1	pyrroline-5-carboxylate reductase	-
ACNOHD_RS01505 (ACNOHD_01505)	-	320991..321680 (-)	690	WP_014818746.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACNOHD_RS01510 (ACNOHD_01510)	pilT	321745..322779 (+)	1035	WP_003303153.1	type IV pilus twitching motility protein PilT	Machinery gene
ACNOHD_RS01515 (ACNOHD_01515)	pilU	322840..323985 (+)	1146	WP_014818747.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACNOHD_RS01520 (ACNOHD_01520)	-	323982..324818 (-)	837	WP_179485475.1	TIGR03915 family putative DNA repair protein	-
ACNOHD_RS01525 (ACNOHD_01525)	-	324818..326029 (-)	1212	WP_418643729.1	putative DNA modification/repair radical SAM protein	-
ACNOHD_RS01530 (ACNOHD_01530)	-	326163..326558 (+)	396	WP_003282737.1	NINE protein	-
ACNOHD_RS01535 (ACNOHD_01535)	-	326636..327910 (-)	1275	WP_023446074.1	dihydroorotase	-
ACNOHD_RS01540 (ACNOHD_01540)	-	327910..328926 (-)	1017	WP_418643730.1	aspartate carbamoyltransferase catalytic subunit	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42457.70 Da
• Isoelectric Point: 6.7256

>NTDB_id=1103891 ACNOHD_RS01515 WP_014818747.1 322840..323985(+) (pilU) [Stutzerimonas kunmingensis strain L4]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVSKTAMSPEMTRETVHGVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTIEELKLPDVLKKLSMTKRGLVLFVGATGTGKSTSLASMIGYRNKNSSGHIISIED
PIEFIHQHQNCIVTQREVGIDTSSFEVALKNTLRQAPDVILIGEIRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPPDRHNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTELGMQTFDQA
LYNLYVQGEITYEDALLHADSANDLRLLIKLGSETDGEHLTSVSQGLSLEVSDDDPGRSFR

Nucleotide

• Length: 1146 bp

>NTDB_id=1103891 ACNOHD_RS01515 WP_014818747.1 322840..323985(+) (pilU) [Stutzerimonas kunmingensis strain L4]
ATGGAATTCGAGAAACTTCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGATCTGTTCATCACGGCAGGCGTGCCGCCTTC
GATGAAGGTCAACGGCAAGATCATGCCGGTCAGCAAGACGGCGATGTCGCCGGAAATGACCCGCGAGACCGTGCACGGCG
TTATGAACGAGCAGCAGCGCCGCGAGTTCACCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGTATCGGCCGT
TTCCGGGTCAGTGCGTTCTACCAGCGCAATCTCGCCGGCATGGTGCTGCGGCGTATCGAAACCAACATTCCGACCATCGA
AGAACTCAAGCTGCCGGATGTCCTCAAGAAGCTGTCGATGACCAAGCGCGGGCTGGTGCTGTTCGTCGGGGCCACCGGTA
CGGGCAAGTCGACCTCATTGGCTTCGATGATCGGCTACCGCAACAAGAATTCCAGCGGGCACATCATCTCCATCGAAGAC
CCGATCGAATTCATCCACCAGCATCAGAACTGCATCGTTACCCAGCGCGAGGTCGGTATCGACACCAGCTCGTTCGAAGT
GGCGCTGAAGAACACCTTGCGCCAGGCGCCGGACGTAATCCTCATCGGCGAGATCCGTACCCGTGAAACCATGGACTACG
CCGTGGCCTTCGCCGAAACCGGTCACCTGTGCCTGGCGACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGCCCGACCGTCACAACCAGGTGTGGATGGATCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGACGCCTGACGGCAAGGGGCGTCGTGCGGTGATCGAGGTGCTGATCAATACGCCGCTGGCGGCCGACCTGA
TCCGCAAGGGCGAGGTGCATGAGCTCAAGTCGCTGATGAAGCGCTCCACCGAGCTGGGCATGCAGACCTTCGACCAGGCG
CTGTACAACCTCTATGTGCAGGGTGAAATCACCTACGAAGATGCGTTGCTGCACGCCGATTCGGCCAACGATTTGCGTCT
GCTGATCAAGCTGGGATCGGAAACCGATGGCGAGCATCTGACCAGCGTGTCCCAGGGCCTGAGCCTTGAGGTCAGTGACG
ACGATCCAGGCCGCAGCTTCCGCTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

3D structure

• AlphaFold DB: I4CN99

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	95.276	100	0.953
pilU	Acinetobacter baylyi ADP1	66.106	93.701	0.619
pilU	Vibrio cholerae strain A1552	59.714	91.864	0.549
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.658	88.976	0.388