Detailed information NTG0110295

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: ACAK32_RS17870
• Genome accession: NZ_AP031580
• Coordinates: 3731875..3732933 (-)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain JUNP419
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3726875..3737933

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACAK32_RS17845 (JUNP419_3582)	aroK	3727145..3727714 (-)	570	WP_000643712.1	shikimate kinase AroK	-
ACAK32_RS17850 (JUNP419_3583)	pilQ	3727726..3729891 (-)	2166	WP_001017033.1	type IV pilus secretin PilQ family protein	Machinery gene
ACAK32_RS17855 (JUNP419_3584)	pilP	3729959..3730486 (-)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
ACAK32_RS17860 (JUNP419_3585)	pilO	3730497..3731237 (-)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
ACAK32_RS17865 (JUNP419_3586)	pilN	3731234..3731875 (-)	642	WP_000201227.1	PilN domain-containing protein	Machinery gene
ACAK32_RS17870 (JUNP419_3587)	pilM	3731875..3732933 (-)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
ACAK32_RS17875 (JUNP419_3588)	ponA	3733096..3735651 (+)	2556	WP_000736677.1	penicillin-binding protein PBP1a	-
ACAK32_RS17880 (JUNP419_3589)	-	3735669..3736493 (-)	825	WP_002019666.1	putative RNA methyltransferase	-
ACAK32_RS17885 (JUNP419_3590)	-	3736496..3736828 (-)	333	WP_000169594.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=110295 ACAK32_RS17870 WP_002014066.1 3731875..3732933(-) (pilM) [Acinetobacter baumannii strain JUNP419]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=110295 ACAK32_RS17870 WP_002014066.1 3731875..3732933(-) (pilM) [Acinetobacter baumannii strain JUNP419]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCCGATCGTCTTG
CGAATCCAAATCGTGTAAATGTACTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGCCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTCTTTGGTGGCAAGCAACTTACGCTTGAAATTCAAAGTCGTTATGGCTTGTCTTTAGAAGAA
GCAAGCCGAGCGAAAAAAGATCGCTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGTTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTAGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423